Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Effect of Various Garlic Extracts on Hyperlipidemia Rats Induced by Triton WR-1339

추출조건을 달리한 마늘 추출물이 Triton WR-1339 유발 고지혈증 흰주의 체내 지질대사에 미치는 영향

  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University) ;
  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Han, Choong-Ho (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 정명수 (원광대학교 한의과대학) ;
  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 한중호 (원광대학교 한의학전문대학원) ;
  • 이기남 (원광대학교 한의학전문대학원)
  • Published : 2009.04.30
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Abstract

The purpose of this study was to investigate the effect of garlic(Allium sativum L.) extracts with extraction conditions on hyperlipidemia prevention. The extracts prepared for garlic by hot temperature extraction(HG), low temperature extraction(LG), UMPM extraction(UG), fermentation(FG) and black garlic hot temperature(BG) method. To evaluate of hypolipidemia effect in vivo, we examined serum and liver lipid profiles of Triton WR-1339 induced hyperlipidemia rats. Serum triglyceride and total cholesterol levels in UG group were significantly higher than control group. Liver total cholesterol content in LG group and liver triglyceride in UG group was significantly lower than control group.

Keywords

References

  1. Korea National Statistical Office : Annual report of mortality statistics. Seoul ; 2005.
  2. Ministry of Health and Welfare : Report on 2005 National Health and Nutrition Survey. Seoul ; 2006.
  3. Culter RG : antioxidants aging and longevity. In Free Radicals in Biology. Pryor WA, ed. Academic Press, New York, USA. 1984 ; 6 : 371-423.
  4. Kwon SK : Organosulfur compounds from Allium sativum L. and physiological activities. J Appl Pharmacol. 2003 ; 11 : 8-32.
  5. Huh J : Jeuungbo Dogneubogam. Namsandang, Seoul. 1981 ; 1172.
  6. Kim CM, Shin MK, Ahn DG, Lee KS : The encyclopedia of oriental herbal medicine. Jeung Dam Pub. 2003
  7. Choi HK : A study on the antibacterial activity of garlic against Escherichia coli 0157. J Korean Practical Arts Edu. 2001 ; 14 : 159-167.
  8. Lemar KM, Turner MP and Lloyd D : Garlic(Allium sativum) as an anti-candida agent : a comparison of the efficacy of fresh garlic and freeze-dried extracts. J Appl Microbiol. 2002 ; 93 : 398-405. https://doi.org/10.1046/j.1365-2672.2002.01707.x
  9. Yin MC and Tsao SM : Inhibitory effect of seven Allium plants upon three Aspergillus species. Int. J Food Microbiol. 1999 ; 49 : 49-56. https://doi.org/10.1016/S0168-1605(99)00061-6
  10. Ruffin J, Hunter SA : An evaluation of the effect of garlic as an antihypertensive agent. Ctyobios. 1983 ; 37 : 85-89.
  11. Ackermann RT, Mulrow CD, Ramirez G, Gardner CD, Morbidoni K and Lawrence VA : Garlic shows promise for improving some cardiovascular risk factors. Arch Intern Med. 2001 ; 161 : 813-824. https://doi.org/10.1001/archinte.161.6.813
  12. Banerjee S and Maulik S : Effect of garlic on cardiovascular disorder. review. Nutr J . 2002; 1: 4. https://doi.org/10.1186/1475-2891-1-4
  13. Kim ES, Chun HJ : The anticarcinogenic effect of garlic juice against DMBA induced carcinoma on the hamster buccal pouch (in korean). J Korean Soc Food Nutr. 1993 ; 22 : 398-404.
  14. Takezaki T, Gao CM, Wu JZ, Ding JH, Liu YT, Zhang Y, Li SP, Su P, Liu TK and Tajima K: Dietary protective and risk factors for esophageal and stomach cancers in a low-epidemic area for stomach cancer in Jiangsu Province, China : Comparison with those in a high-epidemic area. Jpn J Cancer Res. 2001 ; 92 : 1157-1165. https://doi.org/10.1111/j.1349-7006.2001.tb02135.x
  15. Takezaki T, Gao CM, Ding JH, Liu TK, Li MS and Tajima K : Comparative study of ligestyles of residents in high and low risk areas for gastric cancer in Jiangsu province, China : with special reference to Allium vegetables. J Epidemiol. 1999 ; 9 : 297-305. https://doi.org/10.2188/jea.9.297
  16. Iciek MB, Rokita HB and Wlodek LB : Effects of diallyl disulfide and other donors if sulfane sulfur on the proliferation of human hepatoma cell line(Hep G2). Neoplasma. 2001 ; 48 : 307-312.
  17. Lea MA and Randolph VM : Induction of histone acetylation in rat liver and hepatoma by organosulfur compounds including diallyl disulfide. Anticancer Res. 2001 ; 21 : 2841-2845.
  18. Yamasaki T and Kau BH : Garlic compounds protect vascular endothelial cells from oxidant injury. Nippon Yakurigaku Zasshi. 1997 ; 110(1) : 138-141. https://doi.org/10.1254/fpj.110.supplement_138
  19. Kusama H, Nishiyama M and Ikeda S : Pharmacological investigation of bezafibrate, a hypolipidemic agent(I). Effect of bezafibrate on mormal experimental hyperlipidemia in rats. Folia Pharmacol Japon. 1988 ; 92 : 175-180. https://doi.org/10.1254/fpj.92.175
  20. Jung HJ, Nam JH, Park HJ, Lee KT, Park KK, Kim WB, Choi J : The MeOH extract of Pleurospermum kamtschaticum and its active component buddlejasaponin (IV) inhibits intrinsic and extrinsic hyperlipidemia and hypercholesterolemia in the rat, J Ethnopharmacol. 2007 ; 112(2) : 255- 61. https://doi.org/10.1016/j.jep.2007.03.012
  21. Oh PS, Lee SJ, Lim KT: Hypolipidemic and antioxidative effects of the plant glycoprotein( 36kDa) from Rhus verniciflua stokes fruit in Triton WR-1339-induced hyperlipidemic mice, Biosci Biotechnol Biochem. 2006 ; 70(2) : 447-56. https://doi.org/10.1271/bbb.70.447
  22. Lee IA, Lee JH, Baek NI, Kim DH: Antihyperlipidemic effect of crocin isolated from the fructus of Gardenia jasminoides and its metabolite Crocetin, Biol Pharm Bull. 2005 ; 28(11) : 2106-10. https://doi.org/10.1248/bpb.28.2106
  23. 함인혜, 이웅철, 이병희, 최호영: 국산과 중 국산 枳實의 Triton WR-1339 유발 高脂 血症흰쥐에 대한 효능연구, 대한본초학회지, 2007 ; 22(3) : 109-116.
  24. Folch J, Lees M, Stanley GSH: A simple method for the isolation and purification of total lipid from animal tissues, J Bio Chem. 1957 ; 226 : 497-509.
  25. Fauci AS, Braunwald E, Isselbacher KJ, Longo DL: Harrison's principles of intermal medicine 14th ed. McGraw-Hill Hesgpth Professions Division, USA. 1994 ; p.2139.
  26. Catlos T, Goetz WR: Sandritter's color atlas & textbook of Histopathology, 7th ed., Year Book Med. Co., 1984 ; 153-155.
  27. Hayashi H, Shitara M, Yamasaki F : The origin of lipid accumulated in the liver lysosomes after administration of triton WR-1339, J Biochem. 1982 ; 92(5) : 1585- 90.
  28. Venkatesan S, Ward RJ, Peter TJ : Effect of chronic ethanol feeding on the hepatic secretion of VLDL, Biochim Biophys Acta. 1988 ; 960(1) : 61-6. https://doi.org/10.1016/0005-2760(88)90009-4
  29. Robbins SL, Cotran R, Kumar V: Robbins pathologic basis of disease. 4th edition. W.B. Saunders. 1989 ; 556-561.
  30. Dalin R, Hiroyuki N, Hideomi A, Takahiro N: Study on Antihypertensive and Antihyperlipidemic effects of marine algae, Fisheries Science. 1994 ; 60(1) : 83-8.
  31. Hermier D, Hales P, Brindley DN: Effect of lipase inhibitor, Triton WR-1339 and tetrahydrolipstatin, on the synthesis and secretion of lipids by rat hepatocytes, FEBS Lett. 1991 ; 286(1,2) : 186-188. https://doi.org/10.1016/0014-5793(91)80970-E
  32. Gerber LE, Erdman JW JR : Hyperlipidemia in rat fed retinoic acid, Lipids. 1981 ; 16(7) : 496-501. https://doi.org/10.1007/BF02535047
  33. Kozarsky KF, McKinley DR, Austin LL, Raper SE, Stratford-Perricaudet LD, Wilson JM: In vivo correction of low density lipoprotein receptor deficiency in the Watanabe heritable hyperlipidemic rabbit with recombinant adenoviruses, J Bio Chem. 1994 ; 269(18) : 695-702.
  34. Noriaki K, Toru K, Atsushi M, Masayuki Y, Kenji I, Yutaka N, Chuichi K: Induction of mRNA for LDL receptors in heterozygous Watanabe Heritable Hyperlipidemic Rabbit treated with CS-514(Pravastatin) and Cholestyramine, Circulation. 1989 ; 79(5) : 1084-1090. https://doi.org/10.1161/01.CIR.79.5.1084
  35. Vushkin ML, Dolgov AV: Activity of cholesterol metabolism enzymes and lipid levels in the rat liver, aorta, adrenals and serum after exposure to Triton WR-1339, Vopr Med Khim. 1986 ; 32(3) : 98-101.
  36. 방혜정, 강윤호: 고지혈증의 처방에 관한 문헌적 고찰, 동서의학, 1996 ; 21(2) : 70-81.
  37. Hirsch RL and Keller A : The pathogensis of hyperlipidemia induced by means of surface-active agents. I. Increased total body cholesterol in mice given Triton WR- 1339 parenterally. J Exp. Med. 1956 ; 104 : 1-13. https://doi.org/10.1084/jem.104.1.1
  38. Goldfarb S : Rapid increase in hepatic HMG-CoA reductase activity and in vivo cholesterol synthesis after Triton WR- 1339 injection. J Lipid Res. 1978 ; 19 : 489-494.
  39. Kui NY, Oh HK: Clinical pathology file, 3th ed., EuiHak Publishing & Printing Company, Seoul. 2000 ; pp.95-98, 101-102, 1478- 1482, 1546-1550.
  40. Hue FS, Krook L, Pomd WG, Duncan JR: Interactions of dietary calcium with toxic levels of lead and zinc in pigs, J Nutr. 1975 ; 105 : 112.
  41. Lim YK: Clinical application of biochemical test, Komoonsa, Seoul, 1980.
  42. Anber V, Griffin BA, McConnel M, Packard CJ, Shepherd J : Influence of plasma lipid and LDL-subfraction profile on the interaction between low density lipoprotein with human arterial wall proteoglycan, Atherosclerosis. 1996 ; 124 : 261-271. https://doi.org/10.1016/0021-9150(96)05842-X
  43. Austin MA, Rodriguez BL, McKnight B, McNeely MJ, Edwards KL, Curb JD, Sharp DS: Low-density lipoprotein particle size, triglycerides, and high-density lipoprotein cholesterol as risk factors of coronary heart disease in older Japanese-American men, Am J Cardiol. 2000 ; 86 : 412-416. https://doi.org/10.1016/S0002-9149(00)00956-5
  44. Guerin M, Bruckert E, Dolphin PJ, Turpin G, Chapman MJ : Feno-fibrate reduces plasma cholesterol ester transfer from HDL to VLDL and normalizes the atherogenic, dense LDL profile in combined hyperlipidemia, Arterioscler Thromb Vasc Biol. 1996 ; 16 : 763-772. https://doi.org/10.1161/01.ATV.16.6.763
  45. 박정의: 관상동맥경화증 위험 인자의 역학 연구, 한국지질학회지 제14차 추계학술대회. 1997 ; 7 : 101-108.