The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum

세균성 시들음병에 대한 식물성 유산균(Lactobacillus sp.)의 저해효과

  • Shrestha, Anupama (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Choi, Kyu-Up (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agricultural and Life Sciences, Kangwon National University) ;
  • Cho, Sae-Youll (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University)
  • 아누파마슈레스타 (강원대학교 생물자원공학부 응용생물) ;
  • 최규업 (강원대학교 생물자원공학부 응용생물) ;
  • 임춘근 (강원대학교 생물자원공학부 응용생물) ;
  • 허장현 (강원대학교 자원생물환경학과) ;
  • 조세열 (강원대학교 생물자원공학부 응용생물)
  • Published : 2009.03.31
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Abstract

An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 $(4{\times}10^8 cfu/ml)$ into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.

KLF01으로 명명된 세균이 토마토의 뿌리에서 분리 되었고 생화학적, 유전학적 검증을 통해 Lactobacillus sp.로 동종 되었다. In vitro 실험에서 식물성 세균인 Ralstonia solanacearum를 비롯하여, Xanthomonas axonopodis pv. citri, Xanthomonas campestris pv. vesicatoria, Eriwinia pyrifoliae, Eriwinia carotovora subsp. carotovora group에게도 저해 효과를 나타내는 것으로 확인되었다. 고추와 토마토를 이용한 in vivo 실험에서는 특히 R. solanacearum에 대해 대조구와 비교 시들음병의 진행을 저해하는 것으로 관찰되었다.

Keywords

References

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