Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolated from pigs and cattle

소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성

  • Lee, Woo-Won (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Jung, Byeong-Yeal (National Veterinary Research & Quarantine Service) ;
  • Lee, Gang-Rok (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Lee, Dong-Soo (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Gyeongsang National University)
  • 이우원 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 정병열 (국립수의과학검역원) ;
  • 이강록 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 이동수 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 김용환 (경상대학교 수의과대학)
  • Published : 2009.03.30
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Abstract

At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. Definitive type 193 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. Schwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.

Keywords

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