Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Detection of plcR-papR Genes by PCR in Identifying Enterotoxin Genes-Harboring Bacillus cereus Strains

장독소 유전자 함유 Bacillus cereus 확인을 위한 독소 전사 조절 유전자 plcR-papR의 PCR 검출법

  • Yun, Suk-Hyun (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Kim, Yong-Sang (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • So, Soon-Ku (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Jeong, Do-Yeon (Institute of Sunchang Fermented Soybean Products) ;
  • Hahn, Kum-Su (Institute of Sunchang Fermented Soybean Products) ;
  • Uhm, Tai-Boong (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
  • 윤숙현 (전북대학교 자연과학대학 생물과학부 및 유전공학연구소) ;
  • 김용상 (전북대학교 자연과학대학 생물과학부 및 유전공학연구소) ;
  • 소순구 (전북대학교 자연과학대학 생물과학부 및 유전공학연구소) ;
  • 정도연 (순창장류연구소) ;
  • 한금수 (순창장류연구소) ;
  • 엄태붕 (전북대학교 자연과학대학 생물과학부 및 유전공학연구소)
  • Received : 2009.12.08
  • Accepted : 2009.12.22
  • Published : 2009.12.31
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Abstract

Identification of virulent Bacillus cereus strains was examined by PCR using primers specific for the detection of plcR-papR, which encode regulatory proteins controlling the transcription of virulence factors in B. cereus. Total 96 strains of B. cereus that carried at least one of diarrheal toxin genes including hblACD, nheABC, and cytK showed all positive PCR products, while other 48 Bacillus strains that lacked the toxin genes were plcRpapR-negative. This PCR method targeting the plcR-papR genes appears to be simple and effective in identifying the enterotoxin genes-harboring B. cereus strains.

Bacillus cereus 설사독소 전사조절유전자인 plcR 및 papR의 특이적 프라이머 쌍을 사용한 PCR로 설사 독소유전자 함유 B. cereus 균의 검출 가능성을 조사하였다. 적어도 한 종류 이상의 독소유전자들(hblACD, nheABC, cytK)을 함유한 96균주의 B. cereus를 대상으로 plcR-papR 유전자에 특이적인 프라이머들을 이용하여 PCR을 한 결과 모두 양성을 나타냈다. 그러나 독소 유전자를 함유하지 않은 48균주의 Bacillus들로 같은 분석을 한 결과 모두 음성을 보였다. PCR에 의한 plcR-papR 유전자 검출법은 설사독소 유전자를 함유한 B. cereus를 확인하는데 간편하면서도 정확한 결과를 제공하였다.

Keywords

References

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