Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mechanism Analysis of Effect of Oxygen on Molecular Weight of Hyaluronic Acid Produced by Streptococcus zooepidemicus

  • Duan, Xu-Jie (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Niu, Hong-Xing (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Tan, Wen-Song (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Zhang, Xu (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
  • Received : 2008.01.28
  • Accepted : 2008.07.16
  • Published : 2009.03.31
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Abstract

Dissolved oxygen (DO) has a significant effect on the molecular weight of hyaluronic acid (HA) during the fermentation of Streptococcus zooepidemicus. Therefore, to further investigate the effect of DO on the yield and molecular weight of HA, this study compared the metabolic flux distribution of S. zooepidemicus under aerobic conditions at various DO levels. The metabolic flux analysis demonstrated that the HA synthesis pathway, considered a dependent network, was little affected by the DO level. In contrast, the fluxes of lactate and acetate were greatly influenced, and more ATP was generated concomitant with acetate at a high DO level. Furthermore, the has gene expression and HA synthase activity were both repressed under anaerobic conditions, yet not obviously affected under aerobic conditions at various DO levels. Therefore, it was concluded that the HA molecular weight would seem to depend on the concomitant effect of the generation of ATP and reactive oxygen species. It is expected that this work will contribute to a better understanding of the effect of the DO level on the mechanism of the elongation of HA chains.

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References

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