Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Characteristics of Antibiotic Resistant Bacteria in Urban Sewage and River

도시하수 및 그 주변 하천 환경 중 항생제 내성 세균 노출 특성

  • Oh, Hyang-Kyun (School of Civil and Environmental Engineering, Yonsei University) ;
  • Park, Joon-Hong (School of Civil and Environmental Engineering, Yonsei University)
  • 오향균 (연세대학교 사회환경시스템공학부) ;
  • 박준홍 (연세대학교 사회환경시스템공학부)
  • Received : 2008.12.18
  • Accepted : 2009.03.27
  • Published : 2009.03.31
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Abstract

This research investigated the characteristics of antibiotic resistance of bacteria in microbial communities from municipal wastewater treatment plants (MWTPs), and monitored seasonal changes of antibiotic resistant bacteria (ARB) from MWTPs and Han river. When antibiotics were amended to either R2A agar (R2A) for general heterotrophs or MacConeky sorbitol agar (MSA) for coliform bacteria, all the MWTP samples exhibited multiple antibiotic resistance on the antibiotic-amended solid media. The antibiotic resistance appearing frequencies of ampicillin and sulfathiazole, respectively, were higher than reported data for other countries. The antibiotic resistance appearances differed depending upon the concentrations of primary substrate and nutrients and the types of cultivation media. The following 16S rRNA gene phylogenetic analysis showed that the identified multiple-antibiotic resistant microbes on R2A plates were more likely to be known human-pathogenic bacteria than the background heterotrophic bacteria were, suggesting a high risk of antibiotic resistance appearance to public health. In addition, according to our investigation of seasonal changes of ARB from urban MWTP and river samples, the frequency of ARB appearances was shown to correlate positively with temperature. This indicates a possibility that global warming result in increase in microbial risk to public health.

본 연구는 도시 하수처리장의 미생물 군집 내 항생제 내성 세균의 특징과 하천으로의 항생제 내성 세균 노출의 계절적 변화의 특성을 평가하였다. 일반 종속영양세균 배양을 위한 R2A agar (R2A)와 대장균군을 선택하여 배양하는 MacConkey sorbitol agar (MSA)에 항생제를 첨가 하여 제작한 배지에 하수처리장 시료를 도말하여 배양한 결과 모든 시료에서 다제 항생제 내성 세균을 검출 해 낼 수 있었다. Ampicillin과 sulfathiazole의 내성률이 다른 나라에 비해 높게 측정 되었으며 시료내 유기물의 정도, 사용된 배지에 따라 내성률이 다름을 볼 수 있었다. R2A 배지에서 분리된 다제 항생제 내성 세균은 모두 기존에 알려진 병원성 세균과 그 염기서열이 유사한 것으로 볼 때 병원성 세균의 항생제 내성 정도가 일반 세균보다 높음을 본 연구 결과로 보일 수 있었다. 또한 본 연구에서는 하수처리장이 하천에 미치는 유해성을 계절별로 관찰하여 전체 미생물중 항생제 내성 세균의 비율은 수온과 비례한다는 결과를 얻었다. 이 결과는 지구 온난화가 미생물 유해성을 증가시킬 가능성을 시사한다.

Keywords

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