Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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A Study on the Optimal Analytical Method for the Determination of Urinary Arsenic by Hydride Generation-Atomic Absorption Spectrometry

HG-AAS법에 의한 요중 비소의 최적 분석법에 관한 연구

  • Lee, Jong-Wha (Department of Environmental Health Science, Soonchunhyang University) ;
  • Lee, Ui-Seon (Department of Environmental Health Science, Soonchunhyang University) ;
  • Hong, Sung-Chul (Department of Environmental Health Science, Soonchunhyang University) ;
  • Jang, Bong-Ki (Department of Environmental Health Science, Soonchunhyang University)
  • 이종화 (순천향대학교 환경보건학과) ;
  • 이의선 (순천향대학교 환경보건학과) ;
  • 홍성철 (순천향대학교 환경보건학과) ;
  • 장봉기 (순천향대학교 환경보건학과)
  • Received : 2009.08.25
  • Accepted : 2009.10.22
  • Published : 2009.10.31
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Abstract

This study was carried out to examine the optimal analytical method for determination of urinary toxic arsenic (inorganic arsenic and its metabolites) by HG-AAS (hydride generation-atomic absorption spectrometry). In the analysis of SRMs (standard reference materials), method E (addition of 0.4% L-cysteine to pre-reductant and use 0.04M HCl as carrier acid) showed the most accurate results compared with the reference values. In the analysis of 30 urinary samples, analytical results were significantly different depend on the component of pre-reductant and the concentration of carrier acid. When the concentration of carrier acid was higher, the analytical result was lower. The recovery rates of MMA (monomethylarsonic acid) and DMA (dimethylarsenic acid) were varied by the concentration of pre-treatment acid and carrier acid and hydride generation reagents. When the concentration of carrier acid was 1.62 M (5% HCl), the recovery rates of DMA was 1%. The recovery rates of MMA and DMA in method E (=V) were 102% and 100%, respectively. The results of this study suggest that the component and concentration of pre-reductant and carrier acid must be carefully adjusted in the analysis of urinary arsenic, and method E is recommendable as the most precise analytical method for determination of urinary toxic arsenic.

Keywords

References

  1. Murphy, E. A., Aucott, M. : An assessment of the amounts of arsenical pesticides used historically in a geographical area. Science of the Total Environment, 218, 89-101, 1998 https://doi.org/10.1016/S0048-9697(98)00180-6
  2. Jeong, K. C. : Occupational diseases. Seoul, Korea Medicine Press, 435, 1999
  3. Jones, F. T. : A broad view of arsenic. Poultry Science, 86, 2-14, 2007 https://doi.org/10.1093/ps/86.1.2
  4. Kee, H. S., Sohn, E. H., Park, Y. C., Yu, I. J., Maeng, S. H., Chung, H. W. : An anti-clastogenic role of selenium in arsenic- and chromium-induced oxidative stress causing chromosomal damages. Korean Journal of Environmental Health, 23(4), 9-15, 1997
  5. Mandal, B. K., Suzuki, K. T. : Arsenic round the world: a review. Talanta, 58, 201-235, 2002 https://doi.org/10.1016/S0039-9140(02)00268-0
  6. McSheehy, S., Szpunar, J. : Speciation of arsenic in edible algae by bi-dimensional size-exclusion anion excha nge H PLC with d ual ICP-MS a nd e lectrospra y MS/MS detection. Journal of Analytical Atomic Spectrometry, 1, 79-87, 2000
  7. Brima, E. I., Jenkins, R. O., Haris, P. I. : Understanding arsenic metabolism through spectroscopic determination of arsenic in human urine. Spectroscopy, 20, 125-151, 2006 https://doi.org/10.1155/2006/759046
  8. Hata, A., Endo, Y., Nakajima, Y., Ikebe, M., Ogawa, M., Fujitani, N., Endo, G. : HPLC-ICP-MS speciation analysis of arsenic in urine of Japanese subjects without occupational exposure. Journal of Occupational Health, 49, 217-223, 2007 https://doi.org/10.1539/joh.49.217
  9. ATSDR (Agency for Toxic Substances and Disease Registry). Available from : http:// www.atsdr.cdc.gov/cercla/07list.html. Accessed October 25, 2008
  10. Lindberg, A. L., Goessler, W., Grandér, M., Nermell,B., Vahter, M. : Evaluation of the three most commonly used analytical methods for determination of inorganic arsenic and its metabolites in urine. Toxicology Letters, 168(3), 310-318, 2006 https://doi.org/10.1016/j.toxlet.2006.10.028
  11. The National Institute of Environmental Research :Evaluation of exposure levels to environmental pollutants and biomonitoring of biological samples in Sihwa·Banwol and Ulsan area. Annual Report, 2007, 2008
  12. Ministry of Environment of the Republic of Korea :Survey on health status of residents in the closed mine in Choongnam and Choongpook area. Final report, Ministry of Environment, 2008
  13. Le, X. C., Cullen, W. R., Reimer, K. J. : Effect of cysteine on the speciation of arsenic by using hydride generation atomic absorption spectrometry. Analytica Chimica Acta, 285, 277-285, 1994 https://doi.org/10.1016/0003-2670(94)80066-9
  14. Shraim, A., Chiswell, B., Olszowy, H. : Speciation of arsenic by hydride generation-atomic absorption spectrometry(HG-AAS) in hydrochloric acid medium. Talanta, 50, 1109-1127, 1999 https://doi.org/10.1016/S0039-9140(99)00221-0
  15. Carrero, P., Malave, A., Burguera, J. L., Burguera, M., Rondon, C. : Determination of various arsenic species by flow injection hydride generation atomic absorption spectrometry; Investigation of the effects of the acid concentration of different reaction media on the generation of arsines. Analytica Chimica Acta, 438, 195-204, 2001 https://doi.org/10.1016/S0003-2670(01)00796-6
  16. Song, M . J., Pa rk, K. S ., K im, Y. M ., L ee, W. :Effects and optimum conditions of pre-reductant in the analysis of inorganic arsenic by hydride generation-atomic absorption spectrometry. Analytical Science & Technology, 18(5), 396-402, 2005
  17. Feng, Y. L., Chen, H. Y., Tian, L. C., Na ra saki, H. :Off-line separation and determination of inorganic arsenic species in natural water by high resolution inductively coupled plasma mass spectrometry with hydride generation combined with reaction of arsenic(V) and L-cysteine. Analytica Chimica Acta, 375, 167-175, 1998 https://doi.org/10.1016/S0003-2670(98)00285-2
  18. ACGIH : TLVs a nd BEIs; Arsenic a nd soluble inorganic compounds. 2001
  19. Arbouine, M. W., Wilson, H. K. : The effect of seafood consumption on the assessment of occupational exposure to arsenic by urinary arsenic speciation measurements. Journal of Trace Elements and Electrolytes in Health and Disease, 6, 153-160, 1992
  20. Larsen, E. H., Pritzl, G., Hansen, S. H. : Speciation of eight arsenic compounds in human urine by highperformance liquid chromatography with inductively coupled plasma mass spectrometric detection using antimonate for internal chromatographic standardization. Journal of Analytical Atomic Spectrometry, 8, 557-563, 1993 https://doi.org/10.1039/ja9930800557
  21. Larsen, E. H., Pritzl, G., Hansen, S. H. : Arsenic speciation in seafood samples with emphasis on minor constituents; An investigation using high-performance liquid chromatography with detection by inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry, 8, 1075-1084, 1993 https://doi.org/10.1039/ja9930801075
  22. Buchet, J. P., Pauwels, J., Lauwerys, R. : Assessment of exposure to inorganic arsenic following ingestion of marine organisms by volunteers. Environmental Research, 66, 44-51, 1994 https://doi.org/10.1006/enrs.1994.1043
  23. Branch, N., Ebdon, L., O'Neill, P. : Determination of arsenic species in fish by directly coupled high-performance liquid chromatography-inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry, 9, 33-37, 1994 https://doi.org/10.1039/ja9940900033
  24. Park, J. D. : Safety evaluation of arsenic. Final report, Korea Food & Drug Administration, GOVP1200508249, 2002
  25. Park, S. O. : Evaluation of the uptake dose of pollutants form major foods in Korea and risk assessment. Annual report of Korea Food & Drug Administration, 2006
  26. Guo, T., Baasner, J., Tsalev, D. L. : Fast automated determination of toxicologically relevant arsenic in urine by flow injection-hydride generation atomic absorption spectrometry. Analytica Chimica Acta, 349, 313-318, 1997 https://doi.org/10.1016/S0003-2670(97)00276-6
  27. Tsalev, D. L., Sperling, M., Welz, B. : Flow-injection hydride generation atomic absorption spectrometric study of the automated on-line pre-reduction of arsenate, methylarsonate and dimethylarsinate and high-performance liquid chromatographic separation of their l-cysteine complexes. Talanta, 51(6), 1059- 1068, 2000 https://doi.org/10.1016/S0039-9140(00)00297-6
  28. Lim, H. S., Lee, S. G. : Effects of acid and prereductant in the analysis of arsenic by hydride generation-atomic absorption spectrometry. Analytical Science & Technology, 13(2), 151-157, 2000
  29. Tseng, C. H., Huang, Y. K., Huang, Y. L., Chung, C.J., Yang, M. H., Chen, C. J., Hsueh, Y. M. : Arsenic exposure, urinary arsenic speciation, and peripheral vascular disease in blackfoot disease-hyperendemic villages in Taiwan. Toxicology and Applied Pharmacology, 206, 299-308, 2005 https://doi.org/10.1016/j.taap.2004.11.022
  30. Hsueh, Y. M., Hsu, M. K., Chiou, H. Y., Yang, M.H., Hua ng, C. C ., Chen, C . J. : U rinary a rsenic speciation in subjects with or without restriction from eafood dietary intake. Toxicology Letters, 133, 83-91, 2002 https://doi.org/10.1016/S0378-4274(02)00087-5
  31. Shraim, A., Cui, X., Li, S., Ng, J. C., Wang, J., Jin, Y., Liu, Y., Guo, L., Li, D., Wang, S., Zhang, R.,Hirano, S. : Arsenic speciation in the urine and hair of individuals exposed to airborne arsenic through coal-burning in Guizhou, PR China. Toxicology Letters, 137, 35-48, 2003 https://doi.org/10.1016/S0378-4274(02)00379-X
  32. Xie, Y. X., Miyamato, H., Kondo, M., Koga, H.,Zhang, A., Ohmichi, M., Inaba, Y., Chiba, M. : Element concentrations in urine of patients suffering from chronic arsenic poisoning. The Tohoku Journal of Experimental Medicine, 193, 229-235, 2001 https://doi.org/10.1620/tjem.193.229
  33. Yamato, N. : Concentration and chemical species of arsenic in human urine and hair. Bulletin of Environmental Contamination and Toxicology, 40, 633-640, 1998 https://doi.org/10.1007/BF01697507

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