Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Spermatogenesis of Cichlasoma managuensis, Cichlidae, Teleost

경골어류 시클리드과 Cichlasoma managuensis의 정자형성과정

  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Kim, Seok (Department of Environmental Medical Biology, Central Research Laboratory, Wonju College of Medicine, Yonsei University) ;
  • Song, Mi-Sook (Department of Central Research Laboratory, Wonju College of Medicine, Yonsei University) ;
  • Joo, Kyung-Bok (Department of Ophthalmic Optics, Chodang University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김석 (연세대학교 원주의과대학 환경의생물학교실, 중앙연구실) ;
  • 송미숙 (연세대학교 원주의과대학 중앙연구실) ;
  • 주경복 (초당대학교 안경광학과) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실)
  • Received : 2009.06.25
  • Accepted : 2009.09.23
  • Published : 2009.09.30
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Abstract

The ultrastructure of spermatogenesis and sperm in Cichlasoma managuensis belonging to Cichlidae was investigated by light and electron microscopes. The testis of C. managuensis contained numerous testicular cysts, and spermatogenesis was synchronized in these testicular cysts. In the case of spermatogonia, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The sperm was formed by loss of cytoplasm. The head of mature sperm was a spherical shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have lateral fins.

경골어류 시클리드과에 속하는 Cichlasoma managuense의 정자형성과정과 정자를 광학현미경과 전자현미경을 이용하여 관찰하였다. Cichlasoma managuense의 정소는 부레와 창자 사이에 위치하고 있었고 장축 2.3 cm, 단축 4 mm 정도의 크기로 살색을 띠고 있었다. 정자형성과정은 정소낭(testicular cyst)에서 이루어 졌으며, 각 정소낭 내에 동일한 분화시기의 생식세포가 분포하고 있었다. 정원세포는 타원형이었고 미토콘드리아의 발달이 현저하였다. 제1정모세포는 원형으로 정원세포보다 크기가 작았고 제2정모세포는 제1정모세포보다 더 작아졌고 핵의 전자밀도는 더 높았다. 정세포의 초기발달시기에는 세포의 크기가 정모세포보다 작아졌고 두부의 전자밀도가 높아졌으며 편모가 형성되기 시작하였고 미토콘드리아는 핵 주변에 분포하였다. 정자완성과정 말기에는 핵의 염색질 응축이 뚜렷하였으며 핵은 세포질 한쪽에 치우쳐 있었고, 미토콘드리아는 편모 주변에 집중되었다. 완전히 성숙한 정자의 경우 두부형태는 구형이었고 두부에서 첨체는 관찰되지 않았으며 편모의 미세소관 배열은 9+2 구조를 이루고 있었다. 또한 편모 양쪽으로 lateral fin이 관찰되었다.

Keywords

References

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