Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Spermatogenesis of Coreoleuciscus splendidus, Cyprinidae, Teleostei

경골어류 잉어과 쉬리(Coreoleuciscus splendidus)의 정자형성과정

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Kim, Seok (Department of Environmental Medical Biology, Central Research Laboratory, Wonju College of Medicine, Yonsei University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 김석 (연세대학교 원주의과대학 환경의생물학교실, 중앙연구실) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소)
  • Received : 2009.07.09
  • Accepted : 2009.09.23
  • Published : 2009.09.30
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Abstract

The ultrastructure of spermatogenesis and sperm in Coreoleuciscus splendidus, belonging to Gobioninae, Cyprinidae was investigated by light and electron microscopes. The testis was located between intestine and air bladder. The size of testis was major axis 1.8 cm, minor axis 3 mm. The testis of C. splendidus contained numerous testicular cysts, and spermatogenesis was non-synchronized in these testicular cysts. In May, the upper area of testis contained with other germ cells and sperm but the lower area of testis contained with matured sperm only. In case of spermatogonia, the nucleus was comparatively large spherical, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The head of matured sperm was a spherical shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm had not lateral fins and 7 outer coarse fibers.

경골어류 잉어과 모래무지아과에 속하는 쉬리(Coreoleuciscus splendidus)의 정자형성과정과 정자의 형태를 광학현미경과 전자현미경을 이용하여 관찰하였다. 쉬리의 정소는 부레와 창자 사이에 위치하고 있었고 장축 1.8 cm, 단축 3 mm 정도의 크기로 어두운 흰색을 띠고 있었다. 정자형성과정은 정소낭(testicular cyst)에서 이루어졌으며, 각 정소낭 내에서 다양한 분화시기의 생식세포가 분포하고 있었으나 번식기인 5월에는 정소의 상부는 다양한 분화시기의 생식세포와 정자가 하부에는 성숙한 정자만 관찰되었다. 정원세포는 전자밀도가 매우 낮고 미토콘드리아의 발달이 현저하였다. 제1정모세포는 원형으로 정원세포보다 크기가 작았고 제2정모세포는 제1정모세포보다 더 작아졌고 핵의 전자밀도는 더 높았다. 정세포의 초기발달시기에는 세포의 크기가 정모세포보다 작아졌고 두부의 전자밀도가 높아졌으며 편모가 형성되기 시작하였고 미토콘드리아는 핵 주변에 분포하였다. 정자완성과정 말기에는 핵의 염색질 응축이 뚜렷하였으며 핵은 세포질 한쪽에 치우쳐 있었고, 미토콘드리아는 편모 주변에 집중되었다. 완전히 성숙한 정자의 경우 두부형태는 구형이었고 두부에서 첨체는 관찰되지 않았으며 편모의 미세소관 배열은 9+2 구조를 이루고 있었다. 또한 편모 양쪽으로 lateral fin은 관찰되지 않았으며 7개의 조대원섬유가 발견되었다.

Keywords

References

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