Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Ultrastructure of the Fertilized Egg Envelope in Cichlasoma managuensis, Cichlidae, Teleost

경골어류 시클리드과 Cichlasoma managuensis의 수정란 난막 미세구조

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Kim, Seok (Central Research Laboratory, Wonju College of Medicine, Yonsei University) ;
  • Joo, Kyung-Bok (Department of Ophthalmic Optics, Chodang University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김석 (연세대학교 중앙연구실) ;
  • 주경복 (초당대학교 안경광학과) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실)
  • Published : 2009.03.31
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Abstract

The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis $1.92{\pm}0.08\;mm$, the minor axis $1.43{\pm}0.04\;mm$. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of $13{\sim}15$ horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.

경골어류 시클리드과에 속하는 Cichlasoma managuense의 수정란 난막구조를 광학현미경과 전자현미경을 이용하여 관찰하였다. 수정란은 약황색의 불투명한 부착성 및 침란성란으로 장타원형이었으며 크기는 장축 $1.43{\pm}0.04\;mm$, 단축 $1.85{\pm}0.03\;mm$였다. 수정란의 동물극쪽에서 수정을 위한 정자의 통로로 생각되는 난문이 위치하고 있었고 위란강은 잘 발달되어 있지 않았다. 수정란 난막의 표면에는 부착성 망상구조물들이 덮고 있었고 난문의 형태는 깔때기 형태였다. 수정란 난막의 단면을 관찰한 결과 2층으로 구성되어 있었으며 외층은 부착성 층, 내층은 전자밀도가 서로 다른 $13{\sim}15$층의 층상구조를 하고 있었다. 수정란의 외형은 전형적인 시클리드과에 속하는 다른 어류와 동일한 형태를 하고 있어 과의 특성을 보이며 이들 수정란 난막에서 표면과 단면의 미세구조는 Cichlasoma managuense만이 가지고 있는 종특이성이 될 수 있기 때문에 경골어류의 종을 분류하는데 형태학적 형질로 사용될 수 있을 것으로 사료된다.

Keywords

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