The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Lonicerae Caulis (LC) on Gene Expression of Human melanoma cells

인동등(忍冬藤)이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향

  • 김대수 (동신대학교 한의과대학 안이비인후피부과교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과교실) ;
  • 강성인 (동신대학교 한의과대학 안이비인후피부과교실)
  • Published : 2009.04.25
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Abstract

Objective : This study was designed to investigate anti-cancer and whitening activities (LC). So it was investigated the effects of LC on proliferation rates of melanoma genetic profile by LC. Methods : The genetic profile for the effect of LC on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysis on these genes were conducted. Total 441 genes were up-regulated and 830 genes down-regulated in cells treated with LC. Genes induced or suppressed by LC were all mainly concerned with basic signalling pathways, which are involved in cell growth, differentiation and migration. Especially, many genes, which are related in apoptosis and cell cycle arrest were up-regulated by treatment with LC, and genes related in cell cycle were down-regulated. Result : The network of total protein interactions were identified by using cytoscape program, and some key molecules, such as BCL2L1, SIN3A, SMAD2 and c-myc that can be used for elucidation of therapeutical mechanism of medicine in the future. Conclusion : These results suggest possibility of LC as addition drug and whitening cosmetics. In addition, it was also suggested that related mechanisms are involved in BCL2L1, SIN3A, SMAD2 and c-myc related signalling pathways.

Keywords

References

  1. 陶弘景集 尙志均點校. 名醫別錄. 北京:人民衛 生出版社. 1986:50-1.
  2. 전국한의과대학교수공편저. 本草學. 서울:永林社. 1991:233-4.
  3. 鄭普變, 辛民敎共編. 鄕藥(生藥)大辭典. 서울:永林社. 1990:938-40.
  4. Kang OH, Choi YA, Park HJ, Lee JY, Kim DK, Choi Sc, Kim TH, Nah YH, Yun KJ, Choi SJ, Kim YH, Bae KH, Lee YM. Inhibition of trypsin-induced mast cell activation by water fraction of Lonicera japonica. Arch Pharm Res. 2004;27(11):1141-6. https://doi.org/10.1007/BF02975120
  5. Kim JA, Kim DK, Kang OH, Choi YA, Park HJ, Choi SC, Kim TH, Yun KJ, Nah YH, Lee YM. Inhibitory effect of luteolin on TNF-αlpha-induced IL-8 production in human colon epithelial cells. Int Immunopharmacol. 2005;5(1):209-17. https://doi.org/10.1016/j.intimp.2004.09.027
  6. Lee JH, Ko WS, Kim YH, Kang HS, Kim HD, Choi BT. Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver. Int J Mol Med. 2001;7(1):79-83.
  7. Tae J, Han SW, Yoo JY, Kim JA, Kang OH, Baek OS, Lim JP, Kim DK, Kim YH, Bae KH, Lee YM. Anti-inflammatory effect of Lonicera japonica in proteinaseactivated receptor 2-mediated paw edema. Clin Chim Acta. 2003;330(1-2):165-71. https://doi.org/10.1016/S0009-8981(03)00017-2
  8. Song et al. Anti-inflammatory Activity of the Major Constituents of Lonicera japonica. Arch Pharm. Res. 1995;18(2):133-5. https://doi.org/10.1007/BF02979147
  9. 이송진 외. 忍冬藤 추출물에 대한 항염증 및 진통작용의 비교. 생약학회지. 1994;25(4):363-67.
  10. 류근호 외. 忍冬藤 추출물의 항천식 효과. 생약학회지. 1999;30(4):377-88.
  11. S Hu, W. Cai, J. Ye, Z. Sun. Influence of medicinal herbs on phagocytosis by bovine neutrophils. Zentralbl. Veterinarmed, Reihe. 1992;39:593-9. https://doi.org/10.1111/j.1439-0442.1992.tb00222.x
  12. S. Ohta, N. Sato, S.H. Tu and M. Shinoda, protective effects of Taiwan crude drugs on experimental liver injuries. Yakugaku Zasshi. 1993;113:870-80. https://doi.org/10.1248/yakushi1947.113.12_870
  13. Schuchter LM, Green R, Fraker D. Primary and metastatic diseases in malignant melanoma of gastrointestinal tract. Curr Opin Oncol. 2000;12:181-5. https://doi.org/10.1097/00001622-200003000-00014
  14. 문구, 정병학, 김병주. 암 동서의 결합치료. 원광대학교출판국:신아출판사. 1999:93-7.
  15. Choi HJ, Yee SB, Park SE, Im E, Jung JH, Chung HY, Choi YH, Kim ND. Petrotetrayndiol A induces cell cycle arrest and apoptosis in SK-MEL-2 human melanoma cells through cytochrome c-mediated activation of caspases. Cancer Lett. 2006;232(2):214-25. https://doi.org/10.1016/j.canlet.2005.02.030
  16. Blanchard AP, Kaiser RJ, Hood LE: High-density oligonucleotide arrays. Biosensors Bioelectron. 1996;11:687-90. https://doi.org/10.1016/0956-5663(96)83302-1
  17. N. Iizuka, M. Oka, H. Yamada-Okabe, N. Mori, T. Tamesa, T. Okada, N. Takemoto, A. Tangoku, K. Hamada, H. Nakayama, T. Miyamoto, S. Uchimura, Y. Hamamoto, Comparison of gene expression profiles between hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma by oligonucleotide microarray data on the basis of a supervised learning method, Cancer Res. 2002;62:3939-44.
  18. Roslev P, King GM. Application of a Tetrazolium Salt with a Water-Soluble Formazan as an Indicator of Viability in Respiring Bacteria. Appl Environ Microbiol. 1993 Sep;59(9):2891-6.
  19. Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, Jones DA, Leachman SA.Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol.Mod Pathol. 2001 Feb;14(2):116-28. https://doi.org/10.1038/modpathol.3880267
  20. Weis S, Llenos IC, Dulay JR, Elashoff M, Martínez-Murillo F, Miller CL.Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology. J Neurosci Methods. 2007 Sep30;165(2):198-209. Epub 2007 Jun 7. https://doi.org/10.1016/j.jneumeth.2007.06.001
  21. Kwon KH, Kim SJ, Kim HJ, Jung HH.Analysis of gene expression profiles in cholesteatoma using oligonucleotide microarray. Acta Otolaryngol. 2006 Jul;126(7):691-7. https://doi.org/10.1080/00016480500475633
  22. Zahurak M, Parmigiani G, Yu W, Scharpf RB, Berman D, Schaeffer E, Shabbeer S, Cope L.Pre-processing Agilent microarray data. BMC Bioinformatics. 2007 May 1;8:142.
  23. Pavlícek A, Hrdá S, Flegr J.Free-Treefreeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999;45(3):97-9.
  24. 대한피부과학회 교과서 편찬위원회 편저. 개정4판 피부과학. 서울:여문각. 2001:694.
  25. 정정영, 김미연. 아틀라스 피부관리학. 서울:도서출판 엠디월드. 2007:51-2.
  26. 김은주, 안성연, 남개원, 이해광, 문성준, 김용민, 오명숙, 김남수, 장이섭, 박성규. 赤松葉 함유 한방화장품이 인체 피부 노화에 미치는 영향 연구. 대한본초학회지. 2006;21(1):25-31
  27. 박성규, 김수남, 이종찬, 김한성, 김연준, 이병곤, 장이섭. 滋陰丹이 피부의 노화에 미치는 영향. 대한본초학회지. 2004;19(1):67-76.
  28. 김은철, 안소영, 홍은숙, 이광화, 김은기, 노경호. 천연물에 포함된 미백성분의 추출 및 미백효과. 한국공업화학협회. 2005;16(3):348-53.
  29. 안봉전, 이진영, 박태순, 편정란, 배호정, 송미애, 백은지, 박정미, 손준호, 이창언, 최경임. 추출조건에 따른 울금의 항산화 미백 효과. 한의학잡지. 2006;14(3):168-72.
  30. 장경희, 노석선. 미백효과가 있는 천연물에 대한 고찰. 대전대학교 한의학연구소 논문집. 2004;13(2):289-302.
  31. 정수현, 조우아, 손준호, 박찬익, 이인철, 안봉전, 손애량, 김세기, 김영선, 정연숙, 강보연, 최은영, 이진태. 율피의 생리활성 및 미백효과를 이용한 화장품신소재에 관한 연구. 대한본초학회지. 2005;20(2):27-33.
  32. 中國醫學科學院藥物硏究所等編. 中藥志. 北京:人民衛生出版社. 1988:256-67.
  33. 現代中醫藥文庫 編輯委員會. 中藥現代硏究與應用. 北京:學苑出版社. 1997:2931-63.
  34. 王本祥 主編. 現代中藥藥理學. 天津:天津科學技術出版社. 1997:204-14.
  35. 李宇彬 主編. 中藥有效成分藥理與應用. 哈你濱:黑龍江科學技術出版社. 1995:110-1.
  36. 박경덕, 이석종, 이원주, 김도원, 정호윤, 조병채. 피부 악성 흑색종의 임상 및 병리조직학적소견. 대한피부과학회지. 2007;45(2):149-58.
  37. 조용찬, 김완, 조은택, 서영호, 황선호, 기영화, 이봉규, 강원유, 정다운, 김삼철, 고향미, 정성광. 불명확한 원발부위의 전이성 대장 악석흑색종 1예. 대한소화기내시경학회지. 2006;32:62-6.
  38. Hughes TR, Mao M, Jones AR, Burchard J, Marton MJ, Shannon KW, Lefkowitz SM, Ziman M, Schelter JM, Meyer MR, et al.: Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer. Nat Biotechnol 2001;19:342-7. https://doi.org/10.1038/86730
  39. 박대중, 이장천. 삼백초(三白草)에탄올추출물의 항산화능 및 피부 미백작용 연구. 대한본초학회지. 2008;23(2):193-202.
  40. Pazos Y, Casanueva FF, Camina JP. "Basic aspects of ghrelin action". Vitam. Horm. 2007;77:89-119. https://doi.org/10.1016/S0083-6729(06)77005-4
  41. Gaur U, Aggarwal BB. "Regulation of proliferation, survival and apoptosis by members of the TNF superfamily".Biochem. Pharmacol. 2003;66(8):1403-8. https://doi.org/10.1016/S0006-2952(03)00490-8
  42. Wajant H, Pfizenmaier K, Scheurich P. "Tumor necrosis factor signaling". Cell Death Differ. 2003;10(1):45-65. https://doi.org/10.1038/sj.cdd.4401189
  43. Locksley RM, Killeen N, Lenardo MJ. "The TNF and TNF receptor superfamilies: integrating mammalian biology". Cell 2001;104(4):487-501. https://doi.org/10.1016/S0092-8674(01)00237-9
  44. Soucek, Laura, Jonathan Whitfield, et al. Modelling Myc inhibition as a cancer therapy. Nature. 2008;455:679-83. https://doi.org/10.1038/nature07260
  45. Hartley JL, Temple GF, Brasch MA. DNA cloning using in vitro site-specific recombination. Genome Res. 2001;10(11):1788-95. https://doi.org/10.1101/gr.143000
  46. Zhang Y, Dufau ML. "Dual mechanisms of regulation of transcription of luteinizing hormone receptor gene by nuclear orphan receptors and histone deacetylase complexes.". J. Steroid Biochem. Mol. Biol. 2003;85(2-5):401-14. https://doi.org/10.1016/S0960-0760(03)00230-9
  47. Fiorito F, Marfe, De Blasio E, Granato GE, Tafani M, De Martino L, Montagnaro, lorio S, Pagnini U.2,3,7,8-tetrachlorodibenzop- dioxin regulates bovine herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members. Apoptosis. 2008 Oct;13 (10):1243-52. https://doi.org/10.1007/s10495-008-0249-y
  48. Wrana JL. "TGF-beta receptors and signalling mechanisms.". Mineral and electrolyte metabolism 1998;24(2-3):120-30. https://doi.org/10.1159/000057359
  49. Ida K, Kobayashi S, Taki T, Hanada R, Bessho F, Yamamori S, Sugimoto T, Ohki M, Hayashi Y. "EWS-FLI-1 and EWS-ERG chimeric mRNAs in Ewing's sarcoma and primitive neuroectodermal tumor". Int J Cancer 1995;63(4):500-4. https://doi.org/10.1002/ijc.2910630407
  50. Peeters P, Raynaud SD, Cools J, Wlodarska I, Grosgeorge J, Philip P, Monpoux F, Van Rompaey L, Baens M, Van den Berghe H, Marynen P. "Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia.". Blood 1997;90(7):2535-40.
  51. Roberts SB, Segil N, Heintz N. "Differential phosphorylation of the transcription factor Octl during the cell cycle.". 1991.