The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

The Effects of Gamijihwang-tang(GJT) on Hyperlipidemia in Rats

가미지황탕(加味地黃湯)이 고지혈증(高脂血症) 관련(關聯) 인자(因子)에 미치는 영향

  • Park, So-Ae (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Jo, Hyun-Kyung (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Yoo, Ho-Ryong (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Kim, Yoon-Sik (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Seol, In-Chan (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • An, Jung-Jo (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University)
  • 박소애 (대전대학교 한의과대학 심계내과학교실) ;
  • 조현경 (대전대학교 한의과대학 심계내과학교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실) ;
  • 안정조 (대전대학교 한의과대학 심계내과학교실)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : Gamijihwang-tang(GJT) has been used as a therapeutic agent for hyperlipidemia in oriental medicine for several years. This study was performed to investigate the effects of GJT on hyperlipidemia in rats using diverse biological methods. Method : Hyperlipidemia was induced by a hyper-lipidemic diet fed for 4 weeks. Total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, and glucose were measured in the serum after an oral administration of GJT. Lipid peroxidase, SOD, catalase, ACAT, and HMG-CoA were measured in liver after oral administration of GJT. Result: 1. GJT showed safety against cytotoxicity and toxicity in liver. 2. GJT significantly decreased rat's body and liver weight. 3. GJT significantly decreased serum total cholesterol and LDL-cholesterol, but increased serum HDL-cholesterol. 4. GJT significantly decreased serum triglyceride and glucose. 5. GJT significantly decreased lipid peroxidation and increased SOD and catalase in liver. 6. GJT significantly decreased ACAT and HMG-CoA reductase of cholesterol manifestation in liver. Conclusions : These results suggest that GJT might be effective in treatment and prevention of hyperlipidemia.

Keywords

References

  1. 국립국어원. 표준국어대사전. 초판, 서울: 두산 동아;1999.
  2. 전국한의과대학심계내과학교실. 심계내과학. 초판. 서울: 군자출판사; 2006, p. 169-73.
  3. 방혜정, 고지혈증에 대한 한의학적 고찰. 동서의학. 1995;20:25-36.
  4. 함인혜, 정성웅, 이경진, 박규하, 최호영. 인진호와 한인진이 Triton WR-1339로 유도된 고지혈증 흰쥐에 미치는 영향. 대한본초학회지. 2005 ;20(1):45-52.
  5. 함인혜, 이웅철, 이병희, 최호영. 국산과 중국산 지실이 Triton WR-1339 유발 고지혈증 흰쥐에 대한 효능 연구. 대한본초학회지. 2007;22(3) :109-16.
  6. 함인혜, 이주영, 윤예진, 양갑식, 태진, 부영민, 김호철. 국산 및 중국산 白首烏의 Triton WR-1339유발 고지혈증 흰쥐에 대한 효능 연구. 대한본초학회지. 2007;22(4):279-86.
  7. 김형철, 김형우, 조수인, 김용성, 이장식, 권정남, 김영균. 단삼이 고지혈증 흰쥐의 혈중 지질변화 와 간 조직에 미치는 영향. 대한본초학회지. 2007 ;22(4):239-45.
  8. 이장천. 가미방풍통성산이 고지방식이 급여 흰쥐의 체지질구성과 항산화능에 미치는 영향. 대한본초학회지. 2005;20(2):69-75.
  9. 박종광, 최학주, 김동희. 가미제습순기탕이 고지혈증을 유발시킨 고혈압백서에서의 혈액학적 변화에 미치는 영향. 대전대학교 한의학연구소 논문집. 2006;15(1)19-31.
  10. 김정수, 김병탁. 시령탕이 고혈압 및 고지혈증에 미치는 영향. 대전대 한의학연구소 논문집. 2005;5(2):319-32.
  11. 申卿熙. 蒼濟證方. 초판. 서울: 三藏苑; 1990, p. 104.
  12. Sato N, Fujii K, Yuge O. In vivo and in vitro sevoflurane-induced lipid peroxidation in guinea-pig liver microsomes. 1994;75(6):366-70. https://doi.org/10.1111/j.1600-0773.1994.tb00376.x
  13. Oyanagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem. 1984;142(2):290-6. https://doi.org/10.1016/0003-2697(84)90467-6
  14. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-6. https://doi.org/10.1016/S0076-6879(84)05016-3
  15. Daniel J. Rader, Helen H. Hobbs. Harrison's Principles of Internal medicine. Section 3 Disorders of Intermediary Metabolism. 16th edition. MIP;2006, p. 2038-40, 2058, 2500-12.
  16. 이귀녕, 권오헌. 임상병리파일. 3판. 서울: 의학문화사; 2003, p. 148-99.
  17. Expert panel on detection, evaluation, and treatment of high blood cholesterol in adults. Executive summary of the third report of the third report of National cholesterol education Program(NECP) expert panel on detection, evalution, and treatment of high blood cholesterol in adults(Adult Treatment Panel III). JAMA. 2001;285:2486-97. https://doi.org/10.1001/jama.285.19.2486
  18. 김영석, 문상관, 고창남, 조기호, 배형섭, 이경섭, 노상령, 조효란, 정위성. 心腦通, 降脂延壽 丹 및 淸心調氣湯의 高脂血症에 대한 實驗的 硏究. 대한한방내과학회지. 1998;19(1):202-18.
  19. 김병철, 이은주, 박치상, 박창국. 단삼음이 고지혈증에 미치는 영향. 대한한방내과학회지. 2000 ;21(1):126-34.
  20. 강윤호, 방혜정. 고지혈증의 치방에 대한 문헌적 고찰. 동서의학. 1996;21(2):70-81.
  21. 신재용. 방약합편해설. 서울: 전통의학연구소; 2000, p. 44-7, 62-8.
  22. 이정호, 김호준, 이명종. 천남성이 고지방식이 로 유발된 비만 흰쥐의 당지질대사 및 조직화학적 변화에 미치는 효과. 대한한의학회지. 2007 ;28(1):211-23.
  23. 全國韓醫科大學本草學敎授 公編著. 本草學. 서울: 永林社; 2000, p. 121-3, 276, 302, 303, 347, 348, 409, 445, 450, 503, 504, 523, 537, 578-81.
  24. 신흥묵. 희첨의 혈관이완 효능과 항산화 동태에 관한 연구. 대한한의학회지. 2000;41(3):77-83.
  25. Baecheon Cha, Eunhee Lee, Miae Noh. Antioxidant Activity of Spatholobus suberectus Dunn. Kor. J. Pharmacogn. 2005;36(1):50-5.
  26. Kumai T, Oonuma S, Kitaoka Y, Tadokoro M, Kobayashi S. Biochemical and morphological characterization of spontaneously hypertensive hyperlipidaemic rats. Clin Exp Pharmacol Physiol. 2003;30(8):537-44. https://doi.org/10.1046/j.1440-1681.2003.03872.x
  27. 한기훈, 이해영, 김광일, 이상학, 정익모, 서흥석. Cardiovascular Update. 초판. 2008;10(4) :32-46.
  28. Am J Cardiol. Relation of the number of metabolic syndrome risk factors with all cause and cardiovascular mortality. 2008;102(6) :689-92. https://doi.org/10.1016/j.amjcard.2008.05.010
  29. 고성경. BMI와 %Fat에 의한 비만도 평가가 고지혈증 진단에 미치는 영향. 운동과학. 2004 ;14(1):21-30.
  30. 윤영숙. 여성의 비만. Background of epidermiology. 대한비만학회지. 2004;13(1):211-24.
  31. 장명래, 이운창, 신홍식, 조주연, 안재억, 김주자. 비만도와 비만유형에 따른 혈압, 혈당 및 혈청지질 및 지단백과의 관계. 가정의학회지. 1994;15(12):1076-87.
  32. Berk PD. Regulatable fatty acid transport mechanism are central to the pathophysiology of obesity, fatty liver, and metabolic syndrom. Hepatology. 2008;48(5):1362-76. https://doi.org/10.1002/hep.22632
  33. Halliwell B. Biochemiacal mechanism accounting for the toxic action of oxygen on living organism. The key role of superoxide dismutase. Cell. Biol. Int. Rep;1978, p. 2, 11. https://doi.org/10.1016/0309-1651(78)90032-2
  34. Freman, BA. and Crapo, JD. Biology of disease: Free radicals and tissue injury. Lab. Invest. 1982;47:412.
  35. 대한가정의학회. 가정의학 임상편. 초판. 서울: 계축문화사;2002, p. 303-9.
  36. 윤건호, 손호영. 고지혈증 치료의 최신지견. 가정의학회지. 2000;21(5):31-6.
  37. Junyent M, Cofan M, Nunez I, Gilbert R, Zambon D, Ros E. Influence of HDL cholestrol on Preclinical Carotid Atherosclerosis in Familial Hypercholesterolemia. Arterioscler Thromb Vasc Biol. 2006;26(5):1107-13. https://doi.org/10.1161/01.ATV.0000218507.95149.42
  38. Arafa HM. Curcumin attenuates diet-induced hypercholesterolemia in rats. Med Sci Monit. 2005;11(7):228-34.
  39. Plant S, Shand B, Elder P, Scott R. Adiponectin attenuates endothelial dysfunction induced by oxidised low-density lipoproteins. Diab Vasc Dis Res. 2008;5(2):102-8. https://doi.org/10.3132/dvdr.2008.017
  40. Mehmet Yalniz, I. Halil Bahcecioglu, Nalan Kuzu, Selman Celebi, Huseyin Ataseven, Bilal Ustundag, Ibrahim H. Ozerean, Kazim Sahin. Amelioration of Steatohepatitis with Pentoxifylline in a Novel Nonalcoholic Steatohepatitis Model Induced by High-Fat Diet. Dig Dis Sci. 2007;52:2380-6. https://doi.org/10.1007/s10620-006-9194-1
  41. Milagro FI, Campion J, Maetinez JA. Weight gain induced by high-fat feeding involves increased liver oxidative stress. Obesity(Silver Spring). 2006 Jul;14(7):1118-23.
  42. Meachler P, Wollheim CB, Bentzen CL, and Nieser E. :Role of the intestinal Acyl-CoA: cholesterol acyltransferase activity in the hyper-response of diabetic rats to cholesterol J. Lipid Res. 1992;33:1475-84.
  43. Gills PJ, Robinson C. and Rathgeb KA.: Regulation of ACAT activity by a cholesterol substrate pool during the progression and regression phases of atherosclerosis implications for drug discovery. Atherosclerosis. 1990;83:177-85. https://doi.org/10.1016/0021-9150(90)90163-D
  44. Paaladinesh Thavendiranathan P, Bagai A, Brookhart AM, Choudhry MK. Primary prevention of cardiovascular diseases with statin theraphy. A meta-analysis of randomized controlled trials. Arch Intern Med. 2006;166:2307-13. https://doi.org/10.1001/archinte.166.21.2307