KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Genetic Diversity and Identification of Korean Elite Soybean Cultivars including Certified Cultivars Based on SSR Markers

한국 콩 보급품종을 포함한 엘리트품종의 SSR마커에 의한 유전적 다양성과 품종판별

  • Jang, Seong-Jin (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Park, Su-Jeong (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Park, Kyeong-Ho (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Song, Hang-Lin (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Cho, Yong-Gu (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Jong, Seung-Keun (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kang, Jung-Hoon (National Academy of Agricultural Science, RDA) ;
  • Kim, Hong-Sig (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 장성진 (충북대학교 농업생명환경대학) ;
  • 박수정 (충북대학교 농업생명환경대학) ;
  • 박경호 (충북대학교 농업생명환경대학) ;
  • 송항림 (충북대학교 농업생명환경대학) ;
  • 조용구 (충북대학교 농업생명환경대학) ;
  • 정승근 (충북대학교 농업생명환경대학) ;
  • 강정훈 (농촌진흥청 국립농업과학원) ;
  • 김홍식 (충북대학교 농업생명환경대학)
  • Published : 2009.06.30
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Abstract

A total of 26 Korean elite soybean cultivars including 21 certified cultivars was assessed to evaluate genetic diversity and to analyze relationship among them based on 15 SSR markers. Fifteen SSR markers generated a total of 201 alleles. Average number of alleles per SSR marker was 13.4 with a range from 8 to 19. Genetic diversity of 26 cultivars estimated by PIC value ranged from 0.782 to 0.931 with an average of 0.874. PIC value of Satt197 was the highest with 0.931 and Satt141 was the lowest with 0.782 among 15 SSR markers. Cluster analysis based on genetic distances classified 26 soybean cultivars into 3 clusters. Cluster I, II and III included 2, 7 and 17 cultivars, respectively. Average genetic diversity within clusters was 0.769 with a range from 0.720 to 0.799. Average genetic diversity between clusters was 0.813 with a range from 0.725 to 0.857. Genetic diversity was higher between clusters than within clusters. Genetic relationship among clusters was near between I and II, and I and III and far between II and III cluster. All of 26 Korean elite soybean cultivars could be identified by using any of 5 combinations of 2 SSR markers with higher PIC value, i.e, $Satt197+Sat_088$, Satt197+Satt245, $Sat_088+Sat_-036$, $Sat_088+Satt245$ and Satt185+Satt245.

우리나라에서 1994년부터 2007년까지 보급된 콩 20개 보급품종과 6개의 유망품종을 포함한 26개의 엘리트품종들을 SSR마커를 이용하여 유전적 다양성과 유연관계를 분석하고, 품종을 판별한 결과를 요약하면 다음과 같다. 1. SSR마커 15개를 이용하여 분석한 결과 총 201개의 대립인자가 확인되었고, 각 유전좌별로 최소 8개(Satt141)에서 최대 19개(Satt197)의 대립인자가 확인되었으며, 마커당 대립인자수는 평균 13.4개이었다. 2. 15개 SSR마커에 의한 국내 콩 엘리트품종들의 유전적다양성 (PIC값)은 평균 0.874이었고 그 범위는 0.931-0.782이었으며, 마커별로는 Satt197이 0.931로 가장 높았고 Satt141이 0.782로 가장 낮았다. 3. SSR마커를 이용한 유전적거리에 의한 군집분석한 결과, 26개 품종이 3개 그룹으로 분류되었으며, I그룹에 2품종(7.7%), II그룹에 7품종(26.9%), 그리고 III그룹에 17품종(65.4%)이 속하였다. 4. SSR마커에 의하여 분류된 3그룹내의 유전적 다양성은 0.720-0.799으로 평균 0.769이었고, 그룹간의 유전적 다양성은 0.725-0.857으로 평균 0.813이었다. 그룹간이 그룹내보다 유전적 다양성이 더 높았으며, 유연관계는 I그룹은 II그룹 및 III그룹과 유전적거리가 가까웠으며, II그룹과 III그룹간은 서로 유전적거리가 다소 멀었다. 5. 다형성이 높은 5개의 SSR마커 중에서 2개 마커를 이용한 5개조합($Satt197+Sat_088$, Satt197+Satt245, $Sat_088+Sat_036$, $Sat_088+Satt245$, Satt185+Satt245)이 선정되었으며, 이중 어느 조합을 사용하여도 26개 엘리트품종 모두의 판별이 가능하였다.

Keywords

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