The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Studies on the Anti-cancer Activity of Chaga Mushroom Extract

차가버섯 추출물의 항암활성에 관한 연구

  • Moon, Byung-Hyuk (Dept. of Oriental Medicine The Graduate School, Dong-Guk University) ;
  • Lee, Won-Chul (Dept. of Oriental Medicine The Graduate School, Dong-Guk University)
  • 문병혁 (동국대학교 한의과대학 내과학교실) ;
  • 이원철 (동국대학교 한의과대학 내과학교실)
  • Published : 2009.07.31
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Abstract

Objectives: This study was to investigate the anti-oxidation and anti-cancer activity of Chaga mushroom extract. Extraction condition optimization and beta-glucan analysis and anti-cancer activity tests were also done. Methods: Optimum extraction conditions for Chaga mushroom extract were at a temperature of $90^{\circ}C$ and 2hrs with 10 times of water. Extraction yield and economics were best under these conditions. Results: Anti-oxidation activity was the highest with the fraction of 100,000 MWCO and $IC_{50}$ value was $13{\mu}g/ml$ and this value was comparable to that of vitamin E, alpha-tocopherol. Among the fractions from various organic solvents, ethyl acetate fraction showed the highest anti-oxidation activity with $IC_{50}$ value of $7{\mu}g/ml$. For anti-cancer activity, chloroform fraction showed little anti-cancer activity and ethyl acetate fraction showed the best anti-cancer activity with $IC_{50}$ $1.5{\mu}g/ml$ for stomach cancer cells. Anti-cancer activities for different molecular weight fractions were the best in the fraction of molecular weight less than 100,000Da, and $IC_{50}$ values for stomach cancer cells and liver cancer cells were 1.7 and $1.4{\mu}g/ml$, respectively. Conclusions: From these results, we can conclude that the extract of Chaga mushroom could be a good source for functional food and natural anti-cancer medicine.

Keywords

References

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