Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

The Comparison of Extraction Process for Enhancement of Immunomodulating Activities of Ulva pertusa kjellman

구멍갈파래의 면역활성 증진을 위한 추출방법 비교

  • Published : 2009.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the immunomodulatory effect of Ulva pertusa kjellman extract after undergoing a low temperature and high-pressure extraction process. First, the extracts obtained under the extraction conditions of 150 MPa and $80^{\circ}C$showed a relatively high antioxidant activity, with 90% super oxide radical activity compared to the extracts from conventional extraction process with water at $100^{\circ}C$. This extract also improved the growth of both human immune B and T cells up to $14.5{\times}10^4$ cells/mL and $14.2{\times}10^4$ cells/mL compared to $9.1{\times}10^4$ cells/mL in adding the extracts from conventional processes. It was found that the extracts obtained at 100 MPa and $60^{\circ}C$ showed better activities in NK cell growth and NO production from macrophage as $11.8{\times}10^2$ cells/mL and 30.0 ${\mu}M$. Overall, the extracts from high pressure and low temperature extraction process had relatively higher immune activation activity, possibly because the low temperature and high pressure extraction process may have higher yields of active compounds and have less damage to useful ingredients from relatively weak marine natural resources, such as Ulva pertusa kjellmann than that from the conventional extraction system.

본 연구는 제주도의 녹조현상의 원인인 구멍갈파래의 활용을 위해 고압추출 공정을 통한 추출물의 면역활성 확인 하였다. 150 MPa, $80^{\circ}C$ 조건에서 Superoxide Radical 소거효과에서는 90% 로 활성도가 제일 높았으며, 면역세포 생육도면에서도 B&T cell, 각 각 $14.2{\times}10^4$ cells/mL $14.5{\times}10^4$ cells/mL 나타나 제일 높았다. 반면, NK cell과 NO 면역능 실험에서는 100MPa, $30^{\circ}C$ 조건에서 각각 $11.8{\times}10^2$ cells/mL, 30.0 ${\mu}M$을 보여 주었다. 결과를 통해 구멍갈파래는 면역활성을 가지며 고압 공정을 통한 추출물의 활성이 일반추출에 비해 높은 것을 알 수 있다. 따라서 해조류 및 다른 유용 자원에서도 고압 공정이 기존의 추출방법보다 높은 활성을 나타낼 것으로 판단되며 기능성 식품이나 기능성 소재로서 사용가능성이 있는 것으로 사료된다.

Keywords

References

  1. Choe M. Dietary fats and cancer. J. Korean. Soc. Food Nutr. 5: 513-518 (1991)
  2. Michael CA, Dominic AS, Anue M. Feasibility of drug screening with panel of human tumor cell lines using a microculture tertrazolium assay. Cancer Res. 48: 589-595 (1998)
  3. Ito K, Hori K. Seaweeds; chemical composition and potential food uses. Food Rev. Int. 5: 101-144 (1989) https://doi.org/10.1080/87559128909540845
  4. Steinmetz KA, Potter JD. Vegetable, fruit, and cancer II mechanism. Cancer Causes Aontril. 2: 427-442 (1991) https://doi.org/10.1007/BF00054304
  5. Ray B, Lahye M. Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvaes, chlorphyta), extraction and chemical composition. Carbohyd. Res. 274: 251-261 (1995) https://doi.org/10.1016/0008-6215(95)00138-J
  6. Ayako Y, Koichi Y, Keiichi O. Iodine distribution in blades of several laminarias grown in the same sea area. Bull. Jpn. Soc. Sci. Fish. 58: 1373-1379 (1999)
  7. Yoo JS, Cheun BS, Kim NG. Determination of Na$^+$ channel blocker in seaweeds. Korean. J. Environ. Biol. 19: 107-112 (2001)
  8. Azuine MA, Goswami UC, Katal JJ. Antimutagenic and anticarcinogenic effect of carotenoids and dietary palm oil. Nutr. Cancer 17: 287-295 (1992) https://doi.org/10.1080/01635589209514198
  9. Newmark HL. Plant phenolics as potential cancer prevention agents, Adv. Extract obtained from seaweeds. Bull. Korean. Fish. Soc. 19: 502-508 (1996)
  10. Sugano Y, Kodama H, Terada I, Yamajaki Y, Noma M. Purification and characterization of a novel enzyme, α-neoagararooligosaccharide hydrolase, from a marine bacterium, Vibrio sp. strain JT0107. J. Bacteriol. 176: 6812-6818 (1994)
  11. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY. Effect of ultrasonification process on enhancement of immuno-stimulatory activity of Ephedra sinica Stapf and Rubus coreanus Miq. Korean J. Plant Res. 16: 109-117 (2004)
  12. Zhang S, Zhu J, Wang C. Novel high pressure extraction technology. Int. J. Pharm. 278: 471-474 (2004) https://doi.org/10.1016/j.ijpharm.2004.02.029
  13. Koo SY, Cha KH, Lee DU. Effects of high hydrostatic pressure of foods and biological system. Food Science and lndustry 40: 23-30 (2007)
  14. Kloareg B, Quatrano RS. Structure of the cell walls of marine algae and ecophysical function of the matrix polysaccharides. Oceanogr. Mar. Biol. 26: 259-315 (1988)
  15. Kwon MC, Kim CH, Na CS, Kwak HG, Kim JC, Lee HY. Comparison of immuno-modulatory regulatory activities of rubus coreanus miquel by ultra high pressure extract process. Korean J. Medical Crop Sci. 6: 398-404 (2007)
  16. Lee SH, Lee HS, Park YS, Hwang B, Kim JH, Lee HY. Screening of immune activation activities in the leaves of Dendropanax morbifrea Lev. Korean J. Medical Crop Sci. 10: 109-115 (2002)
  17. Yueran Z, Rui S, You L, Chunyi G, Zhigang T. Expression of leptin recrptors and reponse to leptin stimulation of human killer cell lines. Biochem. Bioph. Res. Co. 300: 247-252 (2003) https://doi.org/10.1016/S0006-291X(02)02838-3
  18. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200 (1958)
  19. Nishikimi M, Roa NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Bioph. Res. Commun. 46: 849-854 (1972) https://doi.org/10.1016/S0006-291X(72)80218-3
  20. Kim CH, Kwon MC, Qadir SA, Hwang B, Nam JH, Lee HY. Toxicity reduction and improvement of anticancer activities from Rhodiola schalinensis A. Bor by ultra high pressure extracts process. Korean J. Medicinal Crop Sci. 6: 411-416 (2007)
  21. Cho JY, Park JS, Yoo ES, Baik KU, Park MH, Han BH. Effect of ginsenosides from panax gineng on Tnf-$\alpha$ production and T cell proliferation. Yakhak Hoeji 42: 296-301 (1998)
  22. Kim IH, Hyun JW, Lee SH, Ha JM, Ha BJ, Lee JH. Immunological stimulating effects of the marine macroalgae, Ulva lactuca with different solvents. Environ. Mutagen Carcinog. 3: 89-92 (2006)