Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of the Mixed Culture of Bacillus subtilis and Lactobacillus plantarum on the Quality of Cheonggukjang

Bacillus subtilis와 Lactobacillus plantarum의 혼합배양이 청국장의 품질에 미치는 영향

  • Ju, Kyung-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Oh, Nam-Soon (Department of Food Science and Technology, Kongju National University)
  • Published : 2009.08.31
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Abstract

The goal of this study was to improve the quality of cheonggukjang by the optimization of the inoculation methods of the Bacillus subtilis (B. subtilis) and Lactobacillus plantarum (L. plantarum) strains. In order to optimize the mixed cultivation of B. subtilis and L. plantarum, the B. subtilis strain was inoculated into steamed soybeans after cultivation of L. plantarum. Inoculation size of B. subtilis was changed to the simultaneous inoculation method in order to stimulate the growth of the L. plantarum in cheonggukjang. The viable cell count of L. plantarum increased from $2{\times}10^7$ CFU/g to $2-6{\times}10^8$ CFU/g and B. subtilis grew to $9{\times}10^8$ CFU/g. These results showed that 2 strains were successfully able to grow in the steamed soybean for good quality of cheonggukjang by optimization of the inoculation methods. The sensory evaluation indicated that a favorable aroma and overall acceptance of cheonggukjang by the optimized mixed cultivation of B. subtilis and L. plantarum, which was relatively higher than those of cheonggukjang by single strain inoculation of B. subtilis.

B. subtilis 균주와 L. plantarum 균주를 혼합배양하여 청국장을 제조할 때 나타나는 여러 가지 품질에 영향을 주는 인자들을 분석하고 평가하였다. B. subtilis 균주와 L. plantarum을 혼합배양할때 L. plantarum의 생육은 잘 되지 않는다. 따라서 혼합배양시 L. plantarum의 생육을 증가시키기 위하여 먼저 L. plantarum을 배양하고, 일정시간이 경과된 후에 B. subtilis를 접종하면 L. plantarum의 생균수가 $2{\times}10^7$ CFU/g에서 48시간 후에 $6{\times}10^8$ CFU/g까지 증가하였다. 혼합배양시 L. plantarum의 생육을 촉진시키는 또 다른 방법으로 B. subtilis의 초기 접종량을 $3.9{\times}10^2$CFU/g, L. plantarum의 접종량을 $2{\times}10^7$ CFU/g으로 조절하여 동시에 접종하였을 때 24시간 배양 후 B. subtilis의 생균수는 $9{\times}10^8$ CFU/g까지 증가하였으며 L. plantarum의 생균수도 $2{\times}10^7$ CFU/g에서 $6{\times}10^8$ CFU/g으로 증가하였다. 즉, 배양방법의 최적화를 통하여 B. subtilis균주와 L. plantarum 균주의 생육을 성공적으로 유도할 수 있었다. 본 연구의 결과로부터 L. plantarum의 생균수가 증가된 청국장의 아미노태 질소함량은 단일균주를 사용하여 제조한 청국장과 유사하였으며, 암모니아 불쾌취가 적었으며, 풍미와 전체적인 기호도에서 높은 점수를 얻었다. 이러한 결과를 바탕으로 청국장 제조시 L. plantarum 균주를 혼합배양함으로써 기존 청국장의 이취 개선과 품질향상에 기여할 것으로 생각된다.

Keywords

References

  1. Lee JO, Ha SD, Kim AJ, Yuh CS, Bang IS, Park SH. Industrial application and physiological functions of cheonggukjang. Food Sci. Ind. 38: 69-78 (2005)
  2. Kim WK, Choi KH, Kim YT, Park HH, Choi JY, Lee YS, Oh HI, Kwon IB, Lee SY. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from cheonggukjang. Appl. Environ. Microb. 62: 2482-2488 (1996)
  3. Heo S, Lee SK, Joo HK. Isolation and identification of fibrinolytic bacteria from Korean traditional cheonggukjang. J. Korean Soc. Agric. Chem. Biotechnol. 41: 119-124 (1998)
  4. Cho YJ, Cha WS, Bok SK, Kim MU, Chun SS, Choi UK. Production and separation of anti-hypertensive peptide during cheonggukjang fermentation with Bacillus subtilis CH-1023. J. Korean Soc. Appl. Biol. Chem. 43: 247-252 (2000)
  5. Park HG, Lee MH, Yoon SH. Effect of cheonggukjang on lipid contents in rats fed high cholesterol diet. J. Korean Soc. Hyg. Sci. 12: 1-6 (2006)
  6. Kim JI, Kang MJ, Kwon TW. Antidiabetic effect of soybean and cheonggukjang. Korea Soybean Digest 20: 44-52 (2003)
  7. Kim JH, Kim SI, Kim JK, Im DK, Park JH, Lee JU, Byun MW. Effect of green tea powder on the improvement of sensorial quality of cheonggukjang. J. Korean Soc. Food Sci. Nutr. 35: 482- 486 (2006) https://doi.org/10.3746/jkfn.2006.35.4.482
  8. In JP, Lee SK, Ahn BK, Chung IM, Jang CH. Flavor improvement of cheonggukjang by addition of Yucca (Yucca shidigera) extract. Korean J. Food Sci. Technol. 34: 57-64 (2002)
  9. Park WJ, Park HY, Yoo JH, Rhee MS. Effect of Artmisia asiatica Nakaki extract on the flavor of cheonggukjang. Food Eng. Prog. 5: 115-124 (2001)
  10. Rhee YH, Kang MS. Physico-chemical characteristics and $\beta$-galactosidase activity of Lactobacillus plantarum from kimchi. Agr. Chem. Biotechnol. 39: 54-59 (1996)
  11. Ha CG, Cho JK, Chai YG, Heo KC. Isolation and identification of lactic acid bacteria containing superior activity of bile salts deconjugation. Korean J. Food Sci. Anim. Res. 24: 164-170 (2004)
  12. Rhim KH, Kim JG, Han JH. Effect of fermented milk on rats fed by hypercholesterolemic diet. Kor. J. Env. Hlth. Soc. 19: 77-89 (1993)
  13. Yoon TJ, Yoo YC, Kang TB, Lee KH, Kwak JH, Baek YJ, Huh CS, Kim JB. Fermented extracts of Korean mistletoe with Lactobacillus (FKM-110) stimulate macrophage and inhibit tumor metastasis. Korean J. Food Sci. Technol. 31: 838-847 (1999)
  14. Eum BW, Kwak BY, Kim SY, Shon DH, Lee KH. Enhancement of chitooligosaccharides in doenjang (soybean paste) and kanjang (soy sauce) using Bacillus subtilis koji and Rhizopus oryzae koji. Korean J. Food Sci. Technol. 35: 291-296 (2003)
  15. Choi KS, Chung YG, Choi C, Chung HC, Im MH, Choi JD, Lee CW. Microbiology fermentation biotechnology: Lactic acid and alcoholic fermentation of low-salted raw kanjang digestion liquor made from Bacillus subtilis var. globigii and Scopulariopsis brevicaulis inoculated meju. J. Korean Soc. Appl. Biol. Chem. 41: 405-409 (1998)
  16. Choe JS, Yoo SM, Kim HR, Kim JS, Chang CM. Microbiology fermentation biotechnology: Volatile compounds of Chonggugjang prepared by different fermentation method and soybean cultivars. J. Korean Soc. Appl. Biol. Chem. 42: 111-115 (1999)
  17. Prestige L, Gage V, Spizizen J. Protease activitives during the course of sporulation on Bacillus subtilis. J. Bacteriol. 107: 815-823 (1971)
  18. Korea Foods Industry Association. Food code. Moonyongsa Co., Seoul, Korea. pp. 319-320 (2009)
  19. Official Methods for Determination of Water Contamination. Ministry of Environment. Seoul, Korea. pp. 195-197 (2000)
  20. Oh SJ. Characteristics of class II bacteriocins produced by lactic acid bacteria. J. Korean Dairy Technol. Sci. 19: 133-146 (2001)
  21. Ko HS, Cho DH, Hwang SY, Kim YM. The effect of quality improvement by cheonggukjang's processing methods. Korean J. Food Nutr. 12: 1-6 (1999)
  22. Lee BY, Kim DM, Kim KH. Physicochemical properties of viscous substance extracted from cheonggukjang. Korean J. Food Sci. Technol. 23: 599-604 (1991)
  23. Allagheny N, Obanu ZA, Campbell-Platt G, Owens JD. Control of ammonia formation during Bacillus subtilis fermentation of legumes. Int. J. Food Microbiol. 29: 321-333 (1996) https://doi.org/10.1016/0168-1605(95)00069-0