Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidant and Anti-fibrotic Properties of Root Extract of Lythrumsalicaria L. in $CCL_4$-Induced Liver Fibrosis Rat Model

사염화탄소로 섬유화가 유도된 흰쥐 간에서 털부처꽃 뿌리 추출물의 항산화 및 섬유화저해 활성

  • Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Ahn, Tae-Jin (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Geum-Sook (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Young-Ok (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Han, Hee-Sun (National Institute of Crop Science, RDA) ;
  • Seo, Jin-Sook (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Chung, Hae-Young (Department of Pharmacy, Pusan National University) ;
  • Park, Chung-Berm (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cha, Sun-Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Ho-Ki (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Seong, Nak-Sul (International Ginseng and Herb Research Institute)
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 한희선 (국립식량과학원) ;
  • 서진숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정해영 (부산대학교 약학대학) ;
  • 박충범 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박호기 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 성낙술 ((재)금산국제인삼약초연구센터)
  • Published : 2009.08.30
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Abstract

Fifty percent ethanol extract of Lythrum salicaria Linne root (LSR) was tested in vitro on antioxidant activity, and furthermore was investigated on antioxidative and fibrosis protecting activities in $CCL_4$-induced liver fibrosis rat model. Ratio of hepatic GSH/GSSG (reduced glutathione/oxidized glutathione) as bio-parameter of antioxidant level in $CCL_4$ plus LSR-treated rats for 6 weeks significantly increased from 2.8- to 5.7-fold than that of $CCL_4$-treated rats at p < 0.05. Thiobarbituric acid reactive substances (TBARS) contents in $CCL_4$ plus LSR-treated rats ranged from 1.57- to 2.19-fold of normal rats and were lower than those in $CCL_4$ plus silymarin-treated rats ($1.78{\sim}2.46$-fold of normal rats) (p < 0.05). Amounts of hydroxyproline of liver tissue showing the content of total collagen, a parameter of fibrosis, in $CCL_4$ plus LSR-administrated rat livers were $4.9{\sim}8.8{\mu}g$/mg ($-47{\sim}-71%$, compared with that in $CCL_4$-treated rat livers ($16.6{\mu}g$/mg tissue), which were significantly lower than those in $CCL_4$ plus silymarin-administrated rats being $8.4{\sim}11.7{\mu}g$/mg ($-30{\sim}-50%$). This collagen reducing effect of liver tissue in $CCL_4$ plus LSR-treated rats was supported by histological observation using microscopy assay. From the results, we conclude that the root of L. salicaria have efficient antioxidant potential and effective antifibrotic activities.

Keywords

References

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