Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Molecular Cloning and Characterization of the Gene Encoding Cinnamyl Alcohol Dehydrogenase in Panax ginseng C.A. Meyer

고려인삼으로부터 Cinnamyl Alcohol Dehydrogenase 유전자의 분리 및 특성

  • Pulla, Rama Krishna (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Shim, Ju-Sun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Jeong, Dae-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Beom-Soo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
  • 라마 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 심주선 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 김유진 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 정대영 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 인준교 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 이범수 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 양덕춘 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행)
  • Published : 2009.08.30
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Abstract

Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.95), catalyzes the reduction of hydroxycinnamaldehydes to give hydroxycinnamyl alcohols, or "monolignols," the monomeric precursors of lignin. Lignins are important components of cell walls and lignified secondary cell walls play crucial roles in long distance transport of water and nutrients during plant growth and development and in plant defense against biotic and abiotic stresses. Here a cDNA clone containing a CAD gene, named as PgCAD, was isolated from a commercial medicinal plant Panax ginseng. PgCAD is predicted to encode a precursor protein of 177 amino acid residues, and its sequence shares high homology with a number of other plant CADS. The expression of PgCAD in adventitious roots and hairy roots of P. ginseng was analyzed using reverse transcriptase (RT)-PCR under various abiotic stresses such as salt, salicylic acid, wounding and chilling treatment that triggered a significant induction of PgCAD at different time points within 2-48 h post-treatment. This study revealed that PgCAD may help the plants to survive against various abiotic stresses.

Keywords

References

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