Changes of Physiological Activity by Drying Temperature in Leaf of Eriobotrya japonica

건조온도에 따른 비파 잎의 생리활성 변화

  • Eom, Hyo-Jin (Dept. of Oriental Medicine Materials, Dongshin University) ;
  • Kim, Sun-Min (Dept. of Oriental Medicine Materials, Dongshin University) ;
  • Pyo, Byoung-Sik (Dept. of Oriental Medicine Materials, Dongshin University) ;
  • Lee, Kyoung-In (Dept. of Oriental Medicine Materials, Dongshin University)
  • 엄효진 (동신대학교 한약재산업학과) ;
  • 김선민 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 이경인 (동신대학교 한약재산업학과)
  • Published : 2009.09.30

Abstract

In DPPH radical and nitric oxide scavenging ability, the extract of lowest temperature condition(40-15H ; 15 hours at $40^{\circ}C$ for drying) exhibited highest activity. The total phenolic and flavonoid contents of each extracts were found to be $120.4{\sim}193.3\;mg$/g and $86.91{\sim}94.55\;mg$/g respectively. It is shown that 40-15H was the highest content in each compound. In antimicrobial activity, a lower drying temperature conditions were found to be more strong activities in disc diffusion assay and each extracts showed MIC of identical level against every tested microbial strains. However 100-2H(2 hours at $100^{\circ}C$ for drying) was exhibited MIC of slightly low level against some strains. And every extract showed fine cell viabilities($101.7{\sim}122.9%$) against RAW 264.7 cell. In anti-proliferation activity against AGS, each extract showed a similar inhibition activity. However in anti-proliferation activity against HeLa, a lower drying temperature conditions showed more strong activities.

Keywords

References

  1. 한방약리학 교재편찬위원회 (2005) 한방약리학, 290-292, 도서출판 신일상사, 서울
  2. 정보섭, 신민교 (2003) 圖解 鄕藥(生藥)大事典, 610-612, 영림사, 서울
  3. Park, M. Y. (2006) Germplasm evaluation and breeding of Eriobotrya japonica Lindl. Ph.D. Thesis in Department of Horticulture Graduate School Chonnam National University, 4-6
  4. Hamada, A., Yoshioka, S., Takuma, D., Yokota, J., Cui, T., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. (2004) The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biol. Pharm. Bull. 27: 1961-1964 https://doi.org/10.1248/bpb.27.1961
  5. Yokota, J., Takuma, D., Hamada, A., Onogawa, M., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. (2006) Scavenging of reactive oxygen species by Eriobotrya japonica seed extract. Biol. Pharm. Bull. 29: 467-471 https://doi.org/10.1248/bpb.29.467
  6. Koba, K., Matsuoka, A., Osada, K. and Huang, Y. S. (2007) Effect of loquat(Eriobotrya japonica) extracts on LDL oxidation. Food Chemistry. 104: 308-316 https://doi.org/10.1016/j.foodchem.2006.11.043
  7. Chen, J., Li, W. L., Wu, J. L., Ren, B. R. and Zhang, H. Q. (2008) Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat(Eriobotrya japonica(Thunb.) Lindl.). Phytomedicine. 15: 98-102 https://doi.org/10.1016/j.phymed.2006.12.014
  8. Banno, N., Akihisa, T., Tokuda, H., Yasukawa, K., Taguchi, Y., Akazawa, H., Ukiya, M., Kimura, Y., Suzuki, T. and Nishino, H. (2005) Anti-inflammatory and antitumor-promoting effect of the triterpene acid from the leaves of Eriobotrya japonica. Biol. Pharm. Bull. 28: 1995-1999 https://doi.org/10.1248/bpb.28.1995
  9. Cho, Y. S., Park, S. K. and Lee, H. Y. (1991) Composition of free sugars, organic acids and free amino acids in loquat flesh. J. Korean Soc. Food Nutr. 20: 89-93
  10. Lee, B. Y., Park, E. M., Kim, E. J., Choi, H. D., Kim, I. H. and Hwang, J. B. (1996) Analysis of chemical components of korean loquat(Eriobotrya japonica Lindl.) fruit. Korean J. Food Sci. Technol. 28: 428-432
  11. Bae, Y. I. and Shim, K. H. (1998) Nutrition components in different parts of korean loquat(Eriobotrya japonica Lindl.). Korean J. Postharvest Sci. Technol. 5: 57-63
  12. Bae, Y. I., Chung, Y. C. and Shim, K. H. (2002) Antimicrobial and antioxidant activities of various solvent extract from different parts of loquat(Eriobotrya japonica Lindl.). Korean J. Food Preserv. 9: 97-101
  13. Bae, Y. I., Jeong, C. H. and Shim, K. H. (2005) Antioxidative and antimicrobial activity of epicatechin isolated from leaves of loquat(Eriobotrya japonica). J. Food Sci. Nutr. 10: 118-121 https://doi.org/10.3746/jfn.2005.10.2.118
  14. Soung, D. Y., Kim, J. S., Chung, H. Y., Jung, H. A., Park, J. C. and Choi, J. S. (1999) Flavonoids and chlorogenic acid from Eriobotrya japonica scavenge peroxynitrite. Natural Product Sciences. 5: 80-84
  15. Bae, Y. I., Jeong, C. H. and Shim, K. H. (2002) Nitrite-scavenging and antimutagenic effects of various solvent extract from different parts of loquat(Eriobotrya japonica Lindl.). Korean J. Food Preserv. 9: 92-96
  16. Lee, K. I. and Kim, S. M. (2009) Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. J. Korean Soc. Food Sci. Nutr. 38: 267-273 https://doi.org/10.3746/jkfn.2009.38.3.267
  17. Amerine, M. A. and Ough, C. S. (1980) Method for analysis of musts and wine, 176-180, Wiley & Sons, Chichester
  18. Lee, Y. C., Hwang, K. H. and Han, D. H. (1997) Composition of Opuntia ficusindia. Korean J. Food Sci. Technol. 29: 847-853
  19. Ku, K. M., Kim, K. U., Lee, S.C. and Kang, Y. H. (2006) Screening of cancer preventive activities from medicinal plants. Korean J. Medicinal Crop Sci. 14: 590-591
  20. Marcocci, L., Maguire, J. J., Droy-Lefaix, M. T. and Packer, L. (1994) The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem. Biophys. Res. Commun. 201: 748-755 https://doi.org/10.1006/bbrc.1994.1764
  21. Collins, C. H., Lyne, P. M. and Grange, J. M. (1995) Collins and Lyne's microbiological methods, 178-205, Butterworth-Heinemann Ltd., Oxford
  22. Shin, K. M., Park, Y. M., Kim, I. T., Hong, S. P., Hong, J. P. and Lee, K. T. (2003) In vitro antiinflammatory activity of amygdalin in murine macrophage Raw264.7 cells. Kor. J. Pharmacogn. 34: 223-227
  23. Liu, R. H. (2004) Potential synergy of phytochemicals in cancer prevention : mechanism of action. J. Nutr. 3479S-3485S
  24. Manach, C., Williamson, G., Morand, C., Scalbert, A. and Remesy, C. (2005) Bioavailability and bioefficacy of polyphenols in humans. Am. J. Clin. Nutr. 81: 230S-242S https://doi.org/10.1093/ajcn/81.1.230S
  25. Ryu, S. W., Jin, C. W., Lee, H. S., Lee, J. Y., Sapkota, K., Lee, B. G., Yu, C. Y., Lee, M. K., Kim, M. J. and Cho, D. H. (2006) Changes in total polyphenol, total flavonoid contents and antioxidant activities of Hibiscus cannabinus L. Korean J. Medicinal Crop Sci. 14: 307-310 https://doi.org/10.1007/s10725-004-4500-4
  26. Dewick, P. M. (2002) Medicinal natural products, 149-151, Wiley & Sons, Chichester
  27. Jo, H. W. and Park, J. C. (2008) Phenolic compounds isolated from the leaves of Angelica keiskei showing DPPH radical scavenging effect. Kor. J. Pharmacogn. 39: 146-149
  28. Yin, Y., Heo, S. I., Jung, M. J. and Wang, M. H. (2009) Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Kor. J. Pharmacogn. 40: 1-5
  29. Chon, S. U. (2008) Antioxidant activity and cytotoxicity of methanol extracts from aerial parts of several edible weed species. Kor. J. Weed Sci. 28(2): 171-180