Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Neuroprotective Activities of Some Medicinal Plants against Glutamate-induced Neurotoxicity in Primary Cultures of Rat Cortical Cells

  • Won, Jin-Bae (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Choong-Je (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
  • Published : 2009.09.30
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Abstract

Neurodegenerative diseases such as Alzheimer's disease, stroke, and Parkinson's disease, are caused by neuronal cell death. Apoptosis, oxidative stress, inflammation, excitotoxicity or ischemia are discussed to play a role of neuronal cell death. In order to find the candidate of neuroprotective agent, neuroprotective activity of some medicinal plants was investigated with in vitro assay system using glutamate-induced neurotoxicity in primary cultures of rat cortical cells. The aqueous methanolic extracts of twenty-seven medicinal plants were evaluated the protective effects against glutamate-injured excitotoxicity in rat cortical cells at the concentration of 50 $\mu$g/ml and 100 $\mu$g/ml, respectively. Among them, extracts of Lonicera japonica, Taraxacum platycarpum, Polygonum aviculare, Gardenia jasminoides, Forsythia viridissima, Lygodium japonicum, Panax notoginseng, Akebia quinata, Anemarrhena asphodeloides and Phellodendron amurense showed significantly neuroprotective activities against glutamate-induced neurotoxicity in primary rat cortical cells.

Keywords

References

  1. Cacabelos, R., Rodriquez, B., Carrera, C., Caamano, J., Beyer, K., Lao, J.I., and Sellers, M.A., APOE-related frequency of cognitive and noncognitive symptoms in dementia. Meth. Find Exp. Clin. Pharmacol., 18, 693-706 (1996)
  2. Hashioka, S., Klegeris, A., and McGeer, P.L., Proton pump inhibitors exert anti-inflammatory effects and decrease human microglial and monocytic THP-1 cell neurotoxicity. Exp. Neurol., 217, 177-183 (2009) https://doi.org/10.1016/j.expneurol.2009.02.002
  3. Isomoto, H., Nishi, Y., Kanazawa, Y., Shikuwa, S., Mizuta, Y., Inoue, K., and Kohno, S., Immune and inflammatory responses in GERD and lansoprazole., J. Clin. Biochem. Nutr. 41, 84-91 (2007) https://doi.org/10.3164/jcbn.2007012
  4. Jeong, J.J., Ha, Y.M., Jin, Y.C., Lee, E.J., Kim, J.S., Kim, H,J., Seo, H.G., Lee, J.H., K.S., and Chang, K.C., Rutin from Lonicera japonica inhibits myocardial ischemia/reperfusion-induced apoptosis in vivo and protects H9c2 cells against hydrogen peroxide-mediated injury via ERK1/2 and PI3K/Akt signals in vitro. Food Chem. Toxicol., 47, 1569-1576 (2009a) https://doi.org/10.1016/j.fct.2009.03.044
  5. Jeong, E.J., Ma, C.J., Lee, K.Y., Kim, S.H., Sung, S.H., and Kim, Y.C., KD-501, a standardized extract of Scrophularia buergeriana has both cognitive-enhancing and antioxidant activities in mice given scopolamine. J. Ethnopharmacol., 121, 98-105 (2009b) https://doi.org/10.1016/j.jep.2008.10.006
  6. Kim, S.R., Hwang, S.Y., Jang, Y.P., Park, M.J., Markelonis, G.J., Oh, T.H., and Kim, Y.C., Protopine from Corydalis ternata has antiacetylcholinestrase and antiamnesic activities. Planta Med., 65, 218-221 (1999) https://doi.org/10.1055/s-1999-13983
  7. Kim, S.R., Koo, K.A., Sung, S.H., Ma, C.J., Yoon, J.S., and Kim, Y.C., Iridoids from Scrophularia buergeriana attenuate glutamate-induced neurotoxicity in rat cortical cultures. J. Neurosci. Res., 74, 948-955 (2003) https://doi.org/10.1002/jnr.10828
  8. Kim, Y.C., Kim, S.R., Markelonis, G.J., and Oh, T.H., Ginsenoside Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration. J. Neurosci. Res., 53, 426-432 (1998) https://doi.org/10.1002/(SICI)1097-4547(19980815)53:4<426::AID-JNR4>3.0.CO;2-8
  9. Lee, J.M., Zipfel, G.J., and Choi, D.W., The changing landscape of ischaemic brain injury mechanisms. Nature, 399(Suppl 6738), 7-14 (1999) https://doi.org/10.1038/399a007
  10. Lee, K.Y., Sung, S.H., and Kim, Y.C., Neuroprotective bibenzyl glycosides of Stemona tuberosa roots. J. Nat. Prod., 69, 679-681 (2006) https://doi.org/10.1021/np0504154
  11. Lin L.M., Zhang X.G., Zhu J.J., Gao H.M., Wang Z.M., and Wang, W.H., Two new triterpenoid saponins from the flowers and buds of Lonicera japonica. J. Asian. Nat. Prod. Res., 10, 925-929 (2008) https://doi.org/10.1080/10286020802217366
  12. Lipton, S.A. and Rosenberg, P.A., Excitatory amino acids as a final common pathway for neurologic disorders. N. Eng. J. Med., 330, 613-622 (1994) https://doi.org/10.1056/NEJM199403033300907
  13. Matsuda, H., Yamazaki, M., Naruto, S., Asanuma, Y., and Kubo, M., Anti-androgenic and hair growth promoting activities of Lygodii Spora (Spore of Lygodium japonicum) I. active constituents inhibiting testosterone 5α-reductase. Biol. Pharm. Bull., 25, 622-626 (2002) https://doi.org/10.1248/bpb.25.622
  14. Michaelis, E.K., Molecular biology of glutamate receptors in the central nervous system and their role in excitotoxicity, oxidative stress and aging. Prog. Neurobiol., 54, 369-415 (1998) https://doi.org/10.1016/S0301-0082(97)00055-5
  15. Muraoka, S. and Miura, T., Inactivation of cholinesterase induced by nonsteroidal anti-inflammatory drugs with horseradish peroxidase: Implication for Alzheimer’s disease. Life Sci., 84, 272-277 (2009) https://doi.org/10.1016/j.lfs.2008.12.006
  16. Rajendra, W., Armugam, A., and Jeyaseelan, K., Neuroprotection and peptide toxins. Brain Res. Brain Res. Rev., 45, 125-141 (2004) https://doi.org/10.1016/j.brainresrev.2004.04.001
  17. Sucher, N.J., Awobuluyi, M., Choi, Y.B., and Lipton, S.A., NMDA receptors: from genes to channels. Trends Pharmacol. Sci., 17, 348-355 (1996) https://doi.org/10.1016/S0165-6147(96)10046-8
  18. Ye, W., Fan, C., Zhang, L., Yin, Z., and Zhao, S., A new phenolic glycoside from the roots of Lygodium japonicum. Fitoterapia, 78, 600-601 (2007) https://doi.org/10.1016/j.fitote.2007.04.003