The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-carcinogenetic and Anti-metastatic Effects of Extract from Maekmoondong-tang in HepG2 Cells

간암 세포주 HepG2에 대한 맥문동탕(麥門冬湯) 추출물의 항암 및 항전이 효능

  • Cheon, Myeong-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Chun, Jin-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Yoon, Tae-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, A-Yeong (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong-Cheol (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Seong-Hwan (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 전명숙 (한국한의학연구원 한약자원연구센터) ;
  • 천진미 (한국한의학연구원 한약자원연구센터) ;
  • 윤태숙 (한국한의학연구원 한약자원연구센터) ;
  • 이아영 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 추병길 (한국한의학연구원한약자원연구센터) ;
  • 김성환 (한국화학연구원 화학유전체실) ;
  • 김호경 (한국한의학연구원 한약자원연구센터)
  • Published : 2009.09.30
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Abstract

Objectives : Maekmoondong-tang (MMDT), a Korean herbal medicine, has been used to treat severe dry cough in patients with bronchitis and pharyngitis. MMDT has been reported to have anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. However, there are no evidence in regard to the effects of MMDT on carcinogenesis and metastasis. Here, we investigated the effects of 70% ethanol extract of MMDT on cell viability, apoptosis, and motility in human hepatocarcinoma HepG2 cells. Methods : Cell viability was measured using the CCK-8 assay, and the apoptosis induction was evaluated by caspase-3 activity. To detect apoptotic features, the cells treated with MMDT were stained with 4'-6-diamidino-2-phenylindole (DAPI). Cell motility was examined by Boyden chamber assay and Real-time Cell Index of Migration assay. Gelatin zymography also performed to measure matrix metalloproteinase (MMP)-2/9 activity. Results : We found that MMDT significantly inhibited cell proliferation and increased caspase-3 activity in a dose-dependent manner in HepG2 cells. Apoptotic features such as chromatin condensation and apoptotic bodies were observed in MMDT-treated cells by DAPI staining. MMDT also suppressed PMA-induced cell motility and activities of MMP-2/9. Conclusions : Our results exhibited that MMDT possess the anti-carcinogenetic and anti-metastatic activities via caspase-3 activation and down-regulation of cell motility and invasion in HepG2 cells. Therefore, these findings suggest that MMDT could be potentially applied to the prevention and treatment of cancer.

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