The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Antitumor Effect of Hang-Am-Dan (HAD) and its Ingredients on Calu6 and MCF-7 Human Cancer Cell Lines

항암단 및 그 주요 성분의 Calu6와 MCF-7 사람 암세포주에 대한 항암효과

  • Lee, Dong-Eun (Radioisotope Research Division, Korea Atomic Energy Research Institute) ;
  • Lee, So-Young (Radioisotope Research Division, Korea Atomic Energy Research Institute) ;
  • Kim, Jung-Sun (East-West Cancer, Dunsan Oriental Hospital, Daejeon University) ;
  • Cho, Chong-Kwan (East-West Cancer, Dunsan Oriental Hospital, Daejeon University) ;
  • Yoo, Hwa-Seung (East-West Cancer, Dunsan Oriental Hospital, Daejeon University) ;
  • Choi, Sun-Ju (Radioisotope Research Division, Korea Atomic Energy Research Institute)
  • 이동은 (한국원자력연구원 동위원소이용기술개발부) ;
  • 이소영 (한국원자력연구원 동위원소이용기술개발부) ;
  • 김정선 (대전대학교 둔산한방병원 동서암센터) ;
  • 조종관 (대전대학교 둔산한방병원 동서암센터) ;
  • 유화승 (대전대학교 둔산한방병원 동서암센터) ;
  • 최선주 (한국원자력연구원 동위원소이용기술개발부)
  • Published : 2009.09.30
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Abstract

Objectives: To elucidate the antitumor activities of Hang-Am-Dan (HAD), we investigated the anti-proliferative effects and related mechanisms of HAD, the main ingredients such as Cordyceps Militaris and Santisigu Tuber, and its main effective components cordycepin and colchicin, respectively. Methods: We cultivated Calu6 and MCF-7 cells and gave them phosphate-buffered saline extracts of HAD, each ingredient of HAD, and the main effective components of each ingredient. After these processes, we performed MTT assay, BrdU assay, TUNEL assay, SDS-PAGE and Western blot analysis and observed the results. Results: The survival rate of these two cancer cells in HAD were 34-38%. The survival rate in extract of Cordyceps militaris (ECM) and extract of Santisigu tuber (EST) were both about 50%. Cordycepin showed decreased survival rate in both cancer cells, 32% and 89%. Colchicin also showed decreased survival rate, 30% and 16%. We observed that all of the cancer cells got apoptotic bodies after adding the extracts and they have more apoptotic bodies when they were exposed to more extracts. The expression of caspase-3 was increased in Calu6 cell lines treated with the ECM, cordycepin and colchicin. The expression of p53 and p21 were increased in the MCF-7 cell lines treated with the ECM and cordycepin. Conclusions: HAD showed cytotoxic activities on the two kinds of human cancer cell lines, Calu6 and MCF-7. Additionally, HAD and its main ingredients caused a dose-dependent inhibition of cell proliferation and induced the apoptotic cell death.

Keywords

References

  1. Lee DK, Kim BS, Lee SG, Gwon HJ, Moon EY, Hwang HS, et al. Momordins inhibit both AP-1 function and cell proliferation. Anticancer Research. 1998;18(1A):119-24.
  2. Park JH, Shon HS, Sung HK, Liu Y, Kim JY, Sung EK, et al. Anti-tumor effect of green tea catechin on cancer cell lines. The Korean J. Anat. 2000;33(4):447-58.
  3. Yoo HS, Son CG, Cho CK. Antitumor Effects of HagnAmDan (HAD) on 55 Patients with Advanced Gastric Cancer. Korean Journal of Oriental Medicine. 2001;2(1):77-88.
  4. Lee YY, Seo SH, Yoo HS, Choi WJ, Cho JH, Lee YW, et al. The clinical study in 83 cases for large intestine cancer patients on the effects by Hangamdan The J. Korean Orient. Oncol. 2000;6(1):165-80.
  5. Yoo HS, Choi WJ, Lee YY, Seo SH, Cho JH, Lee YW, et al. The clinical study in 105 cases for stomach cancer patients on the effects by Hangamdan. J fo daejeon university oriental hospital. 2000;9(2):7-25.
  6. Yoo HS, Lee YY, Song KC, Choi BL, Seo SH, Cho JH, et al. The effects of hangamdan(HAD) on anti-metastasis and preventing relapses, administered to 69 cancer patients. Korean J. Orient. Int. Med. 2002;23(2):165-73.
  7. Kim SD. Study on the anti-metastasis and immune activity of HangAmDan. [dissertation]. Daejeon University Graduate school; 1999.
  8. Ha JW, Yoo HS, Shin JW, Cho JH, Lee NH, Yoon DH, et al. Effects of Cordyceps Militaris Extract on Tumor Immunity. Korean Journal of Oriental Medicine. 2006;27(4):12-29.
  9. Yoo HS, Shin JW, Cho JH, Son CG, Lee YW, Park SY, et al. Effects of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharmacologica Sinica. 2004;25(5):657-65.
  10. Hong SH, Park DI, Seo SH, Choi YH. Antiproliferative effects by aqueous extract of Cordyceps militaris in human leukemic U937 cells. Korean J. Oriental Physiology & Pathology. 2005;19(2):452-8.
  11. Tsujimoto Y. Bcl-2 family: life-or-death switch. Biosci Rep. 2002;22(1):47-58. https://doi.org/10.1023/A:1016061006256
  12. Yoshikawa N, Yamada S, Takeuchi C, Kagota S, Shinozuka K, Kunitomo M, et al. Cordycepin (3’-deoxyadenosine) inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A3 receptor followed by glycogen synthase kinase-3b activation and cyclin D1 suppression. Nauuyn-Schmiedeberg’s Arch Pharmacol. 2008;377(4-6):591-5. https://doi.org/10.1007/s00210-007-0218-y
  13. Lim HW, Kwon YM, Cho SM, Kim JH, Yoon GH, Lee SJ et al. Antitumor activity of Cordyceps militaris on human cancer cell line. Kor. J. Pharmacogn. 2004; 35(4):364-7.
  14. Yeon SH. The chemical composition and biological activities of Cordyceps militaris [dissertation]. Seoul national Univ.; 2000.
  15. Kim SR, Ryu BH, Ryu KW, Park DW. Study of anti-cancer effects of Cremastrae Appendiculatae Tuber on stomach cancer cells. J. Korean Oriental Med. 2001;22(2):75-83.
  16. Borish GG, Taylor EW. The mechanism of action of colchicine-binding of colchine-3H to cellular protein. J. Cell. Biol. 1967;34:533-48.
  17. Goto H, Yano S, Zhang H, Matsumori Y, Ogawa H, Blakey DC et al. Activity of a New Vascular Targeting Agent, ZD6126, in Pulmonary Metastases by Human Lung Adenocarcinoma in Nude Mice. Cancer. Research 2002;62(13):3711-5.