Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Agrobacterium spp. Isolated from Roots of the Crown Gall-infected Grapevine in Chungbuk

충북지방의 뿌리혹병 감염 포도나무 뿌리에서 분리한 Agrobacterium속 균의 특성

  • Yang, Seung-Up (Department of Plant Medicine, Chungbuk National University) ;
  • Park, Se-Jung (Department of Plant Medicine, Chungbuk National University) ;
  • Lee, Young-Kee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
  • 양승업 (충북대학교 식물의학과) ;
  • 박세정 (충북대학교 식물의학과) ;
  • 이영기 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 차재순 (충북대학교 식물의학과)
  • Published : 2009.08.01
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Abstract

The roots of grapevine in the field in which the crown gall was occurred severely in Chungbuk province were collected and Agrobacterium spp. were isolated from the roots using the selective media. The selected 13 isolates were identified as A. tumefaciens with fatty acid analysis using MIDI system, nucleotide sequence of 16S rDNA, biochemical characteristics, and PCR with the species-specific primers. A. vitis, a pathogen of crown gall disease of grapevine was not isolated from the roots. All of the isolates did not show pathogenicity on the tomato seedlings and the stem and root of grapevine. Eric-PCR showed that DNA band patterns of the root isolates were a little more similar to A. tumefaciens than A. vitis. However, overall similarity between the root isolates and the pathogenic strains of A. tumefaciens and A. vitis was low by rep-PCR. These results suggest that a pathogen causing crown gall in grapevine in Chungbuk province may transmitted through the seedlings rather than via soil or roots.

최근 혹병이 심하게 발생한 충북지방의 포도나무의 뿌리로부터 선택배지를 이용하여 Agrobacterium속 세균을 분리하고 동정하였다. 분리균의 지방산 분석을 통한 MIDI에 의한 동정, 16S rDNA 염기서열, 생화학적 특성, 종 특이적 primer을 이용한 PCR 결과로 13개 분리균은 모두 A. tumefaciens로 동정 되었으며, 포도나무 혹병균인 A. vitis는 분리되지 않았다. 모든 분리균은 토마토와 포도나무의 줄기와 뿌리에 병원성을 나타내지 않았다. Rep-PCR 결과 분리균은 A. tumefaciens와 일부 유사성이 있지만 전체적으로 병원균인 A. tumefaciens와 A. vitis와는 유사성이 낮았다. 이상의 결과는 충북 일부 지방의 MBA와 거봉에서 발생한 혹병을 일으키는 병원균은 토양이나 뿌리로부터 전반되지 않고 묘목을 통해서 전반되었을 가능성을 시사하고 있다.

Keywords

References

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