Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Protective Activities of Fractions of Water Extract Obtained from Artemisia iwayomogi Kitamura against Oxidative Stress-induced Mutagenesity: Correlation with Their Reactive Oxygen Scavenging Activity

  • Ahn, Byung-Yong (Department of Oriental Medicine Resources, College of Environmental & Bioresource, Chonbuk National University) ;
  • Jung, Mun-Yhung (Department of Bioscience and Biotechnology in Graduate School, Department of Food Science and Culinary Arts, College of Food Science, Woosuk University) ;
  • Choi, Dong-Seong (Department of Food and Biotechnology, College of Food Science, Woosuk University)
  • Published : 2009.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Water extracts of injinssuk (Artemisia iwayomogi Kitamura) (WE) were obtained from the dried and ground leaves and stems of injinssuk. The WE was further fractionated into crude polysaccharide (C-PS) and nonpolysaccharide fractions (N-PS). The protective activities against the tert-butyl hydro peroxide induced mutangenecity on Escherichia coli PQ37 and reactive oxygen species scavenging activity of the WE, C-PS, and N-PS were studied. The WE obtained from leaves showed a significantly higher inhibitory effect on the mutagenicity than WE from stem. The WE obtained from the leaves having higher crude polysaccharide content but lower content of total carbohydrates had significantly higher antimutagenicity than that from the leaves with lower crude polysaccharide but higher total carbohydrate contents. Further study showed that C-PS fraction showed markedly stronger antimutagenic effect than N-PS. C-PS was also more effective than N-PS for hydroxyl radical scavenging activity, but was similar to N-PS in superoxide radical scavenging activity.

Keywords

References

  1. Simic MG. Mechanisms of inhibition of free-radical prosses in mutagenesis and carcinogenesis. Mutat. Res. 202: 377-386 (1991)
  2. Aeschbacher HU. Antimutagenic/anticarcinogenic food components. pp. 201-216. In: Mutagens and Carcinogens in the Diet. Pariza MW, Aeschbacher H, Felton JS, Sato S (eds). Wiley-Liss, New York, NY, USA (1990)
  3. Yen GC, Duh PD. Antimutagenic effect of methanolic extracts from peanut hulls. Biosci. Biotech. Bioch. 60: 1698-1700 (1996) https://doi.org/10.1271/bbb.60.1698
  4. Yook CS. Artemisia iwayomogi. pp. 524-524. In: Coloured Medicinal Plants of Korea. Academic Press, Seoul, Korea (1989)
  5. Song KW. The phytochemical study of polysaccharide fraction from Artemisia speciess. MS thesis, Sungkyunkwan University, Seoul, Korea (1996)
  6. Koo KA, Kwak JH, Lee KR, Zee OK, Woo ER, Park HK, Youn HJ. Antitumor and immunomodulating activities of the polysaccharide fraction from Artemisia selengensis and Artemisia iwayomogi. Arch. Pharm. Res. 17: 371-374 (1994) https://doi.org/10.1007/BF02974179
  7. Ahn BY, Hwang HS, Baek SW, Kwak JS, Choi DS, Han JH. Desmutagenic effect of water extract from Artemisia iwayomogi Kitamura on the mutagenicity of benzo[a]pyrene. Korean J. Biotechnol. Bioeng. 15: 331-336 (2000)
  8. Urios A, Baanco, M. Specity of spontaneous and t-butyl hydroperoxide-induced mutations in $\Delta$oxy R strains of Escherichia coli differing with respect to the SOS mutagenesis proficiency and to the Mut Y and Mut M functiond. Mutat. Res. 354: 95-101 (1996) https://doi.org/10.1016/0027-5107(96)00043-7
  9. Quillardet P, Hofnung M. The SOS chromotest, a colorimetric bacterial assay for genotoxins: Procedures. Mutat. Res. 147: 65-78 (1985) https://doi.org/10.1016/0165-1161(85)90020-2
  10. Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci. 66: 723-735 (2000)
  11. Ginnopolitis CN, Ries SK. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol. 59: 309-314 (1977) https://doi.org/10.1104/pp.59.2.309
  12. Jung MY, Kim SK, Kim SY. Riboflavin-sensitized photooxidation of ascorbic acid: Kinetics and amino acid effects. Food Chem. 53: 397-403 (1995) https://doi.org/10.1016/0308-8146(95)99834-M
  13. Kim JI, Lee JH, Choi DS, Won BM, Jung MY, Park J. Kinetic study of the quenching reaction of singlet oxygen by common synthetic antioxidants (tert-butylhydroxyanisol, tert-di-butylhydroxytoluene, and tert-butylhydroquinone) as compared with α-tocopherol. J. Food Sci. 74: C362-C369 (2009) https://doi.org/10.1111/j.1750-3841.2009.01160.x
  14. Dubois M, Gilles KA. Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugar and related substances. Anal. Chem. 28: 350-356 (1956) https://doi.org/10.1021/ac60111a017
  15. Maruyama H, Yamazaki K, Murofushi S, Konda C, Ikekkawa T. Antitumor activity of Sarcodon aspratus (Berk.) S. Ito and Ganoderma lucidum (Fr.) Karst. J. Pamacobiodyn. 12: 118-123 (1989) https://doi.org/10.1248/bpb1978.12.118
  16. Gruter A, Friederch U, Wurgler FE. Antimutagenic effects of mushrooms. Mutat. Res. 231: 243-249 (1990) https://doi.org/10.1016/0027-5107(90)90030-8
  17. Zhuang C, Mizuno T, Ito H, Shimura K, Sumiya T, Kawade M. Antitumor activity and immunological property of polysaccharide from the mycelium of liquid cultured Grifola frondosa. Nippon Shouhin Kogyo Gakk. 41: 724-732 (1994) https://doi.org/10.3136/nskkk1962.41.724
  18. Taffe BG, Takahashi N, Kensler TW, Mason RP. Generation of free radicals from organic hydroperoxide tumor promoter in isolated mouse keratinocytes. J. Biol. Chem. 262: 12143-12149 (1987)