KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhanced effect extraction of Antioxidant substance Homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves using Ultrasonic wave system

초음파를 이용한 이대와 왕대 잎으로부터 항산화물질 Homoorientin의 추출효율 향상

  • 이광진 (충북테크노파크 보건의료산업센터) ;
  • 신용국 (충북테크노파크 보건의료산업센터) ;
  • 김영식 (국립강원대학교 삼척캠퍼스 화학공학과)
  • Published : 2009.04.29
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Abstract

In this work, antioxidant substance homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves wereextracted using ultrasonic system, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) on-line ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant screening method. Also, the various experimental variables were the frequency and time of ultrasonic system. From the results, homoorientin was high extracted at the experimental condition of low frequency 35 kHz and time 60 min. And the content of homoorientin in Phyllostachys bambusoides was remarkably higher than that in the Pseudosasa japonica extract.

대나무 (이대와 왕대) 잎으로부터 HPLC on-line ABTS screening기법을 사용하여 flavone C-glycosides의 homoorientin의 항산화활성을 빠르게 분석하였으며, 이대와 왕대 잎으로부터 homoorientin의 추출을 다양한 초음파 에너지와 시간의 추출방법을 적용하였다. 전 처리한 추출액에 포함된 이대와 왕대 잎으로부터 homoorientin을 분석하고 최적의 추출조건을 실험적으로 모색하였다. 실험결과에 의하면 왕대는 이대 잎보다 homoorientin의 함량이 7배 정도 높았으며, 최적 추출은 35 kHz, 60 min에서 (positive) 피크면적 1574.71, 23.67%와 (negativity) 피크면적 6924.34, 1.23%로 왕대 잎에서의 추출 효율이 가장 우수 하였다.

Keywords

References

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