Antimicrobial Activities of Capsella bursa-pastoris Extracts

냉이 추출물의 항균활성

  • Lim, Hyun-Ah (Department of Food Science & Technology Chonbuk national university) ;
  • Yun, Soon-Il (Department of Food Science & Technology Chonbuk national university)
  • Published : 2009.08.30

Abstract

We investigated the natural antimicrobial activities of Capsella bursa-pastoris water, methanol, and ethanol extracts. Antimicrobial activities of these extracts and sodium benzoate on Bacillus cereus, Staphylococcus aureus, and Escherichia coli were compared. The inhibition zone diameters of the methanol and ethanol extracts of Capsella bursa-pastoris were 13-20 mm and 12-20 mm, respectively, which were larger than those of sodium benzoate (11-15 mm). The minimum inhibitory concentrations of Capsella bursa-pastoris methanol and ethanol extracts were 12.5-20 mg/mL. These results indicate that Capsella bursa-pastoris methanol and ethanol extracts can be used as natural antimicrobial agents.

본 연구에서는 냉이의 천연 항균제로서의 사용 가능성을 검토하고자 식중독과 관련된 B. cereus, S. aureus, E. coli 에 대하여 항균활성을 검토하였다. 냉이는 부위별로 에탄올 및 메탄올로 추출한 후 agar diffusion test를 통해 현재 보존료로 사용되고 있는 sodium benzoate와 비교하여 항균활성을 살펴보았다. 그 결과 냉이 잎의 메탄올과 에탄올 추출물은 $15{\sim}20$ mm, $14{\sim}20$ mm, 뿌리의 메탄올과 에탄올 추출물은 $13{\sim}17$ mm, $12{\sim}14$ mm의 저해환을 보여 $11{\sim}15$ mm의 저해환을 보인 Sodium benzoate보다 항균활성이 높게 나타났다. 냉이의 메탄올과 에탄올 추출물의 Minimum inhibitory concentration은 $12.5{\sim}20$ mg/mL로 나타났다. 냉이 추출물의 처리 후 SEM을 통해 세포를 관찰한 결과 세포 표면이 일그러지고 손상을 입은 형태가 관찰되어 추출물 처리시 미생물의 세포벽과 세포막의 기능이 저해되어 항균효과를 나타내는 것으로 추측된다. 따라서 냉이의 메탄올, 에탄올 추출물은 천연 항균제로서의 사용 가능성이 높은 물질로 판단된다.

Keywords

References

  1. Choi, M.S. (2002) Survey on the consumption pattern of the minimally processed fruits and vegetables by urban women. Mastes thesis, ChungAng University, Korea
  2. Chang, T.E., Moon, S.Y., Lee, K.W., Park, J.M., Han, J.S., Song, O. and Shin, I.S. (2004) Mcroflora of manufacturing process and fmal products of Saengshik. Korean J. Food Sci. Technol., 36, 501-506
  3. Kim, S.J. and Park, K.H. (1996) Antimicrobial substances in leek(Allium tuberosum). Korean J. Food Sci. Technol., 28, 604-608
  4. Song, J.H., Kwon, H.D., Lee, W.K. and Park, I.H. (1998) Antimicrobial activity and composition of extract from Smilax china root. Korean J. Soc. Food Sci. Nutr., 27, 574-584
  5. Oh, D.H., Ham, S.S., Park, B.K., Ahn, C. and Yu, J.Y. (1998) Antimicrobial activities of natural medicinal Herbs on food spoilage or foodbome disease tnicroomnism. Korean J. Food Sci Technol., 30, 957-963
  6. Ahn, E.S., Kim, M.S. and Shin D.W (1994) Screening of natuml antimicrobial edible plant extract for Dooboo, fish paste, Makkoli spoilage Microorganism. Korean J. Food Sci. Technol., 26, 733-739
  7. 최영전 (2007) 한국민속식물. 아카데미서적, 서울, 대한민국, p.68
  8. 이동 (1991) 오감으로 찾는 우리풀, 이비, 서울, 대한민국, p64-65
  9. Kwak, JH., Kweon, M.H., Ra, K.S., San, H.C. and Yang,H.C. (1996) Purification and physicochetnical properties of superoxide anion radical scavenger from Capsella bursa-pastoris. Korean J. Food Sci. Technol., 28, 184-189
  10. Choi, H.S., Kang, E.J. and Kim, K.H. (2006) Analyses of essential oil and headspace compositions of CapseZZa bursa-pastons Mdicus by SDE and SPME methods. Korean J. Food Preserv., 13, 108-114
  11. Cho, Y.S., Seo, K.I. and Shim, K.H. (2000) Antimicrobial activities of Korean sword bean(Canavalia gladiata) extracts, Korean J. Postharvest Sci. Technol., 7, 113-116
  12. Lee, Y.S., Seo, K.I. and Shim, K.H. (1999) Antimicrobial activities of chestnut flower extracts (Castanea crenata) Korean J. Postharvest Sci. Technol., 6, 104-109
  13. Farg, R.S. et al (1989) Antimicrobial activity of some Egyptian spice essential oils. J. Food Protect., 52, 665-670
  14. Kong, Y.J., Park, B.K., Oh, DH. (2001) Antimicrobial activity of Quercus mongolica leaf ethanol extract and organic acids against food borne tnicroorganism. Korean J. Food Sci. Technol., 33, 178-183
  15. Hwang, J.S., Chun, H.J. and Han, Y.S. (2000) Isolation and identification of antimicrobial compound from Jakyak (Paeonia japonica var. pilosa NAKAI). Korean J. Soc. Food Sci. 33, 445-452
  16. Park, C.S. (2000) Effect of pine needle and Green Tea extracts on the survival of pathogenic bacteria. Korean J. Food Sci. Technol., 16, 40-46
  17. Lee, Y., Kim, C.H., Sung, T.K, Mun, T.K. and Lim, C.J. (1992) Antibacterial activity of Ulmus pumila. extract. Korean. J. Appl. Microbiol Biotechnol, 20, 1-5
  18. Kwak, Y.K., Yang, J.W. and Lee K.S. (1993) Screening of herb drugs showing antimicrobial activity against some pathogenic microorganism. J. Food Hyg. Safety, 8, 141-145
  19. Prescott, L.M., Harley, J.P. and Klein, D.A. (2002) In Microbiology (5th ed), Antimicrobial chemotherapy. McGraw-Hill Cp., N,Y., USA, 805-822
  20. Cho, S.H., Lee, H.C., Seo, I.W, Kim. Z.U., Chang, Y.s. and Shin, Z.I. (1991) Efficacy of grapefruit seed extract in the preservation of Satsuma mandarin. Korean J. Food Sci. Technol., 23, 164-618