Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Analysis of Anti-Tumor Activity of Flammulina velutipes Extract on B16 cells

B16 흑색종에 대한 팽이버섯 추출물의 항암활성 비교

  • Lee, Si-Rim (Departments of Food Science & Technology Kyungpook National University) ;
  • Nam, Dong-Yun (Departments of Food Science & Technology Kyungpook National University) ;
  • Lee, Hyun-Jin (Departments of Food Science & Technology Kyungpook National University) ;
  • Park, Chul-Hong (Departments of Food Science & Technology Kyungpook National University) ;
  • Heo, Jin-Chul (Departments of Food Science & Technology Kyungpook National University) ;
  • Kim, Jong-Guk (Departments of Food Science & Microbiology Kyungpook National University) ;
  • Lee, Jin-Man (Department of Food & Biotechnology, Hoseo University) ;
  • Lee, Chang-Yun (Green Peace Co., Ltd.) ;
  • Park, Hee-Ju (Green Peace Co., Ltd.) ;
  • Lee, Sang-Han (Departments of Food Science & Technology Kyungpook National University)
  • 이시림 (경북대학교 농업생명과학대학 식품공학과) ;
  • 남동윤 (경북대학교 농업생명과학대학 식품공학과) ;
  • 이현진 (경북대학교 농업생명과학대학 식품공학과) ;
  • 박철홍 (경북대학교 농업생명과학대학 식품공학과) ;
  • 허진철 (경북대학교 농업생명과학대학 식품공학과) ;
  • 김종국 (경북대학교 농업생명과학대학 미생물학과) ;
  • 이진만 (호서대학교 식품생물공학과) ;
  • 이창윤 (그린합명회사) ;
  • 박희주 (그린합명회사) ;
  • 이상한 (경북대학교 농업생명과학대학 식품공학과)
  • Published : 2009.08.30
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Abstract

To evaluate the anti-tumor activity of Flammulina velutipes extract, we used an in vitro wound-healing assay, and an in vivo approach using a mouse melanoma model. Wound-healing activity in B16 cells was affected by the extract in a dose-dependent manner, indicating that the extract had anti-metastatic activity. The extract also exhibited strong anti-tumor activity against lung cancer when B16 cells were injected into mouse veins together with B16 melanoma cells. The results indicatethat the Flammulina velutipes extract decreased B16 cancer cell growth by inhibition of cell migration both in vitro and in vivo.

버섯은 예전부터 단백질, 당질, 비타민, 무기질 등 일반 영양소들이 다량 함유되어 있을 뿐 아니라 다양한 약리작용을 가지고 있기 때문에 예로부터 민식용 및 민간요법으로도 많이 이용되어 왔다(17). 또한 현재에는 건강식으로 다양하게 이용되고 있다. 본 연구는 여러 가지 버섯 중, 팽이버섯의 추출물을 이용하여 이의 항암 활성을 실시하였다. 팽이버섯(Flammulina velutipes)의 항암활성을 검증하기 위하여 in vitro 실험으로 wound healing 활성 실험을 하였으며, in vivo 실험으로 마우스를 이용한 항암모델 실험을 실시하였다. Wound healing 활성 실험 결과 팽이버섯 추출물의 농도를 0-100 ug/mL로 처리함에 따라 B16 세포의 운동성이 감소하는 것을 알 수 있었다. 이를 바탕으로 마우스를 이용하여 항암 모델에 적용한 결과 B16 세포로 인해 형성되는 colony가 팽이버섯 추출물을 처리한 군에서 약 60% 내외로 현저히 감소하는 것을 알 수 있었다. 본 실험 결과는 팽이버섯 추출물의 항암 활성을 확인 한 것으로 향후 이의 추출물을 이용한 세포신호전달과정 등에 관한 연구와 건강기능성식품의 소재로의 제품 개발에 유용하게 사용될 수 있음을 나타내는 주요한 자료로 판단된다.

Keywords

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