Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
권호 표지

Effects of Unloading induced Denervation on Trabecular Bone of Growing Mice; Gender-difference

궁둥신경절제술을 통한 무부하가 성별에 따른 성장기 쥐 해면골에 미치는 영향

  • Seo, Dong-Hyun (Department of Biomedical Engineering & Institute of Medical Engineering, Yonsei University) ;
  • Ko, Chang-Yong (Department of Biomedical Engineering & Institute of Medical Engineering, Yonsei University) ;
  • Kang, Sun-Young (Department of Biomedical Engineering & Institute of Medical Engineering, Yonsei University) ;
  • Lim, Do-Hyung (Silver Technology Center, Korea Institute of industrial Technology) ;
  • Kim, Han-Sung (Department of Biomedical Engineering & Institute of Medical Engineering, Yonsei University)
  • 서동현 (연세대학교 보건과학대학 의공학과 & 의료공학연구원) ;
  • 고창용 (연세대학교 보건과학대학 의공학과 & 의료공학연구원) ;
  • 강순영 (연세대학교 보건과학대학 의공학과 & 의료공학연구원) ;
  • 임도형 (한국생산기술연구원 실버기술개발단) ;
  • 김한성 (연세대학교 보건과학대학 의공학과 & 의료공학연구원)
  • Published : 2009.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate, through a longitudinal tracking, whether gender influences morphological characteristics on trabeucular bone of tibia during unloading. 12 male and 15 female ICR mice at 6 week of age were used and randomly allocated into two groups in each gender; unloading and normal group, respectively Mice in unloading group were operated on denervation (sciatic neurectomy). The tibiae were scanned by using in vivo micro-computed tomography (micro-CT) at 0 day (before denervation) and after 14 days. Structural parameters and BMD on tibia were measured. In female, BV/TV (65%), Tb. Th (5%), Tb.N (61%) and BMD (62%) were significantly decreased and BS/BV (17%), Tb.Sp (54%), SMI (19%) and Tb.Pf (75%) were significantly increased after unloading (p<0.05). In male, BV/TV (1%) and Tb.N (10%) were significantly decreased and SMI (5%) was significantly increased after unloading (p<0.05). In addition, trabeuclar bone loss of female was significantly bigger than that of male (p<0.05). These results indicated that effects of unloading on trabecular bone in growing mice might have difference between female and male, although unloading result in loss of quantity and quality on trabecular bone both female and male.

Keywords

References

  1. Chung, K. Y., "Artifical Gravity; Aerospacemedical Aspect," Journal of Aerospace Medical Center, ROKAF, Vol. 53, No. 1, pp. 3-20, 2006
  2. Cavanagh, P. R. and Rice, A. L., "Bone Loss During Spaceflight," Cleveland Clinic Press, pp. 2-5, 2007
  3. Cavanagh, P. R., Licata, A. A. and Rice, A. J., "Exercise and Pharmacological Countermeasures for Bone Loss During Long-Duration Space Flight," Gravitational and Space Biology, Vol. 18, No. 2, pp. 39-58, 2005
  4. Collet, P., Uebelhart, D., Vico, L., Moro, L., Hartmann, D., Roth, M. and Alexandre, C., "Effects of 1-and 6-Month Spaceflight on Bone Mass and Biochemistry in Two Humans," Bone, Vol. 20, No. 6, p. 547, 1997 https://doi.org/10.1016/S8756-3282(97)00052-5
  5. Ames Research Center, "Final Reports of the U.S. Experiments Flown on the Soviet Biosatellite Cosmos 2044," National Aeronautics and Space Administration, 1994
  6. Morey-Holton, E. R. and Globus, R. K., "Hindlimb Unloading Rodent Model: Technical Aspects," Journal of Applied Physiology, Vol. 92, No. 4, pp. 1367-1377, 2002
  7. Suzue, N., Nikawa, T., Onishi, Y., Yamada, C., Hirasaka, K., Ogawa, T., Furochi, H., Kosaka, H., Ishidoh, K., Gu, H., Takeda, S., Ishimaru, N., Hayashi, Y., Yamamoto, H., Kishi, K. and Yasui, N., "Ubiquitin Ligase Cbl-B Downregulates Bone Formation through Suppression of Igf-I Signaling in Osteoblasts During Denervation," Journal of Bone and Mineral Research, Vol. 21, No. 5, pp. 722-734, 2006 https://doi.org/10.1359/jbmr.060207
  8. Garces, G. and Santandreu, M., "Longitudinal Bone Growth after Sciatic Denervation in Rats," Journal of Bone and Joint Surgery, British Volume, Vol. 70-B, No. 2, pp. 315-318, 1988
  9. Zeng, Q. Q., Jee, W. S., Bigornia, A. E., King, J. G. Jr., D'Souza, S. M., Li, X. J., Ma, Y. F. and Wechter, W. J., "Time Responses of Cancellous and Cortical Bones to Sciatic Neurectomy in Growing Female Rats," Bone, Vol. 19, No. 1, pp. 13-21, 1996 https://doi.org/10.1016/8756-3282(96)00112-3
  10. Bikle, D. D., Sakata, T. and Halloran, B. P., "The Impact of Skeletal Unloading on Bone Formation," Gravitational and Space Biology Bulletin, Vol. 16, No. 2, pp. 45-54, 2003
  11. Järvinen, T. L., Kannus, P., Pajamäki, I., Vuohelainen, T., Tuukkanen, J., Järvinen, M. and Sievänen, H., "Estrogen Deposits Extra Mineral into Bones ofFemale Rats in Puberty, but Simultaneously Seems to Suppress the Responsiveness of Female Skeleton to Mechanical Loading," Bone, Vol. 32, No. 6, pp. 642-651, 2003 https://doi.org/10.1016/S8756-3282(03)00100-5
  12. Hefferan, T. E., Evans, G. L., Lotinun, S., Zhang, M., Morey-Holton, E. and Turner, R. T., "Effect of Gender on Bone Turnover in Adult Rats During Simulated Weightlessness," Journal of Applied Physiology, Vol. 95, No. 5, pp. 1775-1780, 2003 https://doi.org/10.1152/japplphysiol.00455.2002
  13. Lanyon, L. and Skerry, T., "Postmenopausal Osteoporosis as a Failure of Bone's Adaptation to Functional Loading: A Hypothesis," Journal of Bone and Mineral Research, Vol. 16, No. 11, pp. 1937-1947, 2001 https://doi.org/10.1359/jbmr.2001.16.11.1937
  14. David, V., Lafage-Proust, M., Laroche, N., Christian, A., Ruegsegger, P. and Vico, L., "Two-Week Longitudinal Survey of Bone Architecture Alteration in the Hindlimb-Unloaded Rat Model of Bone Loss: Sex Differences," American Journal of Physiology: Endocrinology and Metabolism, Vol. 290, No. 3, pp. E440-E447, 2005 https://doi.org/10.1152/ajpendo.00293.2004