Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Bacillus polyfermenticus KJS-2 as a Probiotic

  • Kim, Kang-Min (Department of Smart Foods and Drugs, Inje University) ;
  • Kim, Myo-Jeong (Department of Smart Foods and Drugs, Inje University) ;
  • Kim, Dong-Hee (Research and Development Center, Daewoo Co. Ltd.) ;
  • Park, You-Soo (Department of Laboratory Biochemistry, Pusan National University School of Medicine) ;
  • Kang, Jae-Seon (Department of Pharmacy, Kyungsung University)
  • Published : 2009.09.30
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Abstract

The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be $60.7{\pm}4.9%$. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of $140{\pm}7.9%$ and $108{\pm}3.5%$, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately $65{\pm}0.6%$.

Keywords

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