FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지

Screening for Antioxidant Effects of Aerial Part Extracts Obtained from Sixteen Compositae Species

국화과 식물 16종 지상부 추출물의 항산화효과 탐색

  • 우정향 (충북대학교 응용생명과학부 원예과학과) ;
  • 신소림 (충북대학교 응용생명과학부 원예과학과) ;
  • 장영득 (충북대학교 응용생명과학부 원예과학과) ;
  • 이철희 (충북대학교 응용생명과학부 원예과학과)
  • Received : 2009.10.07
  • Accepted : 2009.12.26
  • Published : 2009.12.31

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Abstract

The attempts to develop natural antioxidants have been made with aerial part of 16 Compositae species by analyzing their phenolic compound contents, scavenging activities on 1,l-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, ferrous ion chelating effects and inhibition effects on peroxidation of linoleic acids. Total polyphenol and flavonoid contents were highest in Matricaria recutica. Scavenging activity on DPPH and ABTS radicals were highest with extracts of Echinacea angustifolia and Serratula coronata var. insularis f. insularis, respectively. Aerial palt extracts of all species showed lower DPPH scavenging activity than ascorbic acid and 2,6-Di-tert-butyl-4-methylphenol (BHT). But Serratula coronata var. insularis f. insularis demonstrated higher ABTS scavenging activity than ascorbic acid and BHT. In Hieracium pilosella, Echinacea angustifolia, Matricaria reculica extracts showed higher ABTS scavenging activity than BHT. Ferrous ion chelating effects was highest with Matricaria recutica extract, but the effects were much lower than ethylenediaminetetraacetic acid (EDTA). The inhibition activity in lipid peroxidation of linoleic acids was highest in Eupatorium japonicum with 90.06% inhibition 4 days after reaction and 40.52% after 24 days. This demonstrated higher inhibition activity and longer lasting than BHT. Aerial part of Matricaria recutica for extraction source, rather than flower, has higher potential for antiox.idant material. In conclusion, development of natural antioxidants in Compositae is possible by studying antioxidant activity of each species.

본 연구는 국화과 식물 16종 지상부의 페놀성물질 함량, radical 소거능, $Fe^{2+}$ chelating 효과 및 linoleic acid 과산화 억제활성을 분석하여 천연 항산화제 개발에 적합한 식물 소재를 탐색하기 위하여 시행하였다. 연구의 결과, 총 폴리페놀 및 플라보노이드 함량은 저먼캐모마일의 지상부에서 가장 우수하였다. DPPH radical 소거능은 에키나세아 지상부 추출물, ABTS radical 소거능은 산비장이 추출물에서 가장 우수하였다. 15종의 국화과 지상부 추출물은 ascorbic acid와 BHT 보다 DPPH radical 소거능은 낮았으나, 산비장이 추출물의 ABTS radical 소거능은 ascorbic acid와 BHT 보다 우수하였으며 알프스민들레, 에키나세아 및 저먼캐모마일 추출물은 BHT 보다 ABTS radical 소거능이 높았다. $Fe^{2+}$ chelating 효과는 저먼캐모마일 추출물에서 기장 높았으나, EDTA보다 chelating 효과가 현저히 낮았다. 지질과산화 억제활성은 등골나물 추출물에서 기장 높았으며, 반응 4일 후에는 90.06%, 24일 후에는 40.52%의 억제활성을 보여 BHT 보다 지질과산화 억제효과가 높고 오랫동안 억제활성이 유지되었다. 본 연구를 통하여 그동안 꽃을 위주로 사용하였던 저먼캐모마일의 잎줄기가 항산화효과가 우수하다는 것이 밝혀졌으며, 차후 저먼캐모마일 지상부의 활용방안이 증대될 것으로 기대된다. 또한 식물종에 따라 우수한 항산화활성이 각기 다르므로 본 연구결과를 기초로 하여 목적으로 하는 항산화 기능성에 맞는 식물성 항산화제를 개발할 수 있을 것으로 생각된다.

Keywords

Acknowledgement

Supported by : 충북대학교

References

  1. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26:1198-1204.
  2. Cha, J.Y., H.Y. Ahn, K.E. Eom, B.K. Park, B.S. Jun, and Y.S. Cho. 2009. Antioxidative activity of Aralia elata shoot and leaf extracts. J. Life Sci. 19:652-658. https://doi.org/10.5352/JLS.2009.19.5.652
  3. Gonzadez-Perez, O., N.A. Moy-Lopez, and J. Guzman-Muniz. 2008. Alpha-tocopherol and alpha-lipoic acid. an antioxidant synergy with potential for preventive medicine. Rev. Invest. Clin. 60:58-67.
  4. Graf, E. and J.W. Eaton 1990. Anitoxidant functions of phytic acid. Free Rad. Biol. Med. 8:61-69. https://doi.org/10.1016/0891-5849(90)90146-A
  5. Han, W.S. 2003. Isolation and structure elucidation of radical scavengers from Chrysanthemum boreale Makino. Kor. J. Med. Crop Sci. 11:1-4.
  6. Haraguchi, H., K. Hashimoto, and A. Yagi. 1992. Antioxidative substances in leaves of Polygonum hydropiper. J. Agric. Food Chem. 40:1349-1351. https://doi.org/10.1021/jf00020a011
  7. Jang, H.W., H.J. Lee, and K.G. Lee. 2005. Analysis and antioxidant activity of volatile extracts from plants commonly used in Korean foods. Kor. J. Food Sci. Technol. 37:723-729.
  8. Ju, J.C., J.H. Shin, S.J. Lee, H.S. Cho, and N.J. Sung. 2006. Antioxidant activity of hot water extracts from medicinal plants. J. Kor. Soc. Food Sci. Nutr. 35:7-14. https://doi.org/10.3746/jkfn.2006.35.1.007
  9. Jeong, H.Y. 1991. AgingFreeradicalArteriosclerosis. Life Sci. 1:2-14.
  10. Kang, Y.H., Y.K. Oark, S.R. Oh, and K.D. Moon. 1998. Antioxidative property of turmeric (Curumae rhizoma) ethanol extract. Kor. J. Food Thechnol. 30:266-271.
  11. Kawaguchi, K., T. Mizuno, K. Aida, and K. Uchino. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and pseudomonas. Biosci. Biotechnol. Biochem. 61:102-104. https://doi.org/10.1271/bbb.61.102
  12. Kim, E.Y., I.H. Baik, J.H. Kim, S.R. Kim, and M.R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Thechnol. 36:333-338.
  13. Kim, K.B., K.H. Yoo, H.Y. Park, and J.M. Jeong. 2006. Anti-oxidative activities of commercial edible plant extracts distributed in Korea. J. Kor. Soc. Appl. Biol. Chem. 49:328-333.
  14. Kobayasi, Y., Y. Nakano, K. Inayama, A. Sakai, and T. Kamiya, 2003. Dietary intake of the flower extracts of german chamomile (Matricaria recutita L.) inhibited compound 48/80-induced itch-scratch responses in mice. Phytomedicine 10:657-664. https://doi.org/10.1078/0944-7113-00283
  15. Lee, C.B. 2003. Illustrated flora of Korea. Hyangmoonsa. Seoul.
  16. Lee, K.I. and S.M. Kim. 2009. Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. J. Kor. Soc. Food Sci. Nutr. 38:267-273. https://doi.org/10.3746/jkfn.2009.38.3.267
  17. Lee, M.S., S.H. Lee, and K.B. Song. 2004. Effect of various natural antioxidants on the safflower oil. Kor. J. Food Preserv. 11:126-129.
  18. Lee, S.H., I.G. Hwang, J.W. Nho, Y.D. Chang, C.H. Lee, K.S. Woo, and H.S. Jeong. 2009. Quality characteristics and antioxidant activity of Chrysanthemum indicum L., Chrysanthemum boreale M. and Chrysanthemum zawadskii K. powdered teas. J. Kor. Soc. Food. Sci. Nutr. 38:824-831. https://doi.org/10.3746/jkfn.2009.38.7.824
  19. Lee, S.E., J.S. Sung, I.B. Jang, G.S. Kim, T.J. Ahn, H.S. Han, J.E. Kim, Y.O. Kim, C.B. Park, S.W. Cha, Y.S. Ahn, H.K. Park, J.K. Bang, and N.S. Seong. 2008. Investigation on antioxidant activity in plant resources. J. Med. Crop Sci. 16:356-370.
  20. Lee, Y.A., H.Y. Kim, and E.J. Cho. 2005. Comparaison of methanol extracts from vegetables on antioxidative effect under In Vitro and cell system. J. Kor. Soc. Food Sci. Nutr. 34:1151-1156. https://doi.org/10.3746/jkfn.2005.34.8.1151
  21. Liu, H., N. Qiu, H. Ding, and R. Yao. 2008. Polyphenols contents and antioxidant capacity of 68 Chinese harbals suitable for medical or food uses. Food Res. Internal. 41:363-370. https://doi.org/10.1016/j.foodres.2007.12.012
  22. Moon, J.H. and K.H. Park. 1995. Functional components and physiological activity of tea. J. Kor. Tea Soc. 1:175-191.
  23. NFRI. 1990. Manuals of quality characteristic analysis for food quality evaluation (2). National Food Research Institute, Skuba.
  24. Omaye, S.T., K.A. Reddy, and C.E. Cross. 1977. Effect of butylated hydroxy toluene and other antioxidants on mouse lung metabolism. J. Toxicol. Environ. Health 3:829-836. https://doi.org/10.1080/15287397709529617
  25. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  26. Seo, J.S., Y.M. Choi, S.M. Lee, S.H. Kong, and J.S. Lee. 2008. Antioxidant activities and antioxidant compounds of some specialty rices. J. Kor. Soc. Food Sic. Nutr. 37:129-135. https://doi.org/10.3746/jkfn.2008.37.2.129
  27. Shin, J.H., S.J. Lee, J.K. Seo, E.W. Cheon, and N.J. Sung. 2008. Antioxidant activity of hot-water extract from Yuza (Citrus junos Sieb ex Tanaka) Peel. J. Life Sci. 18:1745-1751. https://doi.org/10.5352/JLS.2008.18.12.1745
  28. Velioglu, Y.S., G. Mazza, L. Cao, and B.D. Oomah. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46:4113-4117. https://doi.org/10.1021/jf9801973
  29. Wettasinghe, M. and F. Shahidi. 1999. Antioxidant and free radicalscavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chem. 67:399-414. https://doi.org/10.1016/S0308-8146(99)00137-5
  30. Yen, G.C., P.D. Duhb, and H.L. Tsaia. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and garlic acid. Food Chem. 79:307-313. https://doi.org/10.1016/S0308-8146(02)00145-0
  31. Yoon, M.Y., B.B. Lee, J.Y. Kim, Y.S. Kim, E.J. Park, S.C. Lee, and H.R. Park. 2007. Antioxidant activity and neuroprotective effect of Psoralea corylifolia Linne extracts. Kor. J. Pharmacogn. 38:84-89.