Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Extraction of Quercetin and Its Glucosides from Onion Edible Part Using Solvent Extraction and Various Extraction Assisting Methods

용매 추출과 병용 추출법을 이용한 양파 과육으로부터 quercetin 관련물질의 추출

  • Jin, Eun Young (Department of Agricultural Biotechnology, Seoul National University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Jang, Jae Kweon (Department of Food Science, Chungkang College of Cultural Industries) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University) ;
  • Park, Hoon (Department of Food Science, Sunmoon University) ;
  • Shim, Kun-Sub (Greenbio Co., Ltd.) ;
  • Choi, Young Jin (Department of Agricultural Biotechnology, Seoul National University)
  • 진은영 (서울대학교 농생명공학부) ;
  • 박영서 (경원대학교 식품생물공학과) ;
  • 장재권 (청강문화산업대학 식품과학과) ;
  • 정명수 (이화여자대학교 식품공학과) ;
  • 박훈 (선문대학교 식품과학전공) ;
  • 심건섭 ((주)그린바이오) ;
  • 최영진 (서울대학교 농생명공학부)
  • Received : 2009.02.13
  • Accepted : 2009.05.22
  • Published : 2009.05.31
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Abstract

Quercetin is one of the main flavonoids from onion. To use quercetin as a functional component for onion food products, the effects of various extraction assisting methods such as juicing methods, microwave, ultrasound and enzyme treatments on the yield of quercetin and its glucosides were investigated. For conventional solvent extraction, the highest yield of quercetin and its glycosides was achieved with 0.8 mL/g of 60% methanol at 50$^{\circ}C$ for 15 min. The juicing methods using mixer and screw showed no influence on the yield. Microwave and ultrasound treatments showed 2.14 times and 2.06 times more quercetin yields than non-treated extraction, respectively. For cellulase and viscozyme treatments, the highest yields of quercetin were achieved with 0.5 mL/g of 1% enzyme-0.1M sodium acetate (pH 5.2) buffer solution. Cellulase and viscozyme treatment improved quercetin yield 1.65 times and 2.29 times more than non-treated one, respectively.

양파 과육부에 함유되어 있는 유용 성분인 quercetin과 그 배당체를 다양한 착즙 및 추출방법에 따른 수율을 측정, 분석하였으며 그 결과를 요약하면 다음과 같다. 양파과육 의 quercetin과 배당체의 최적 추출 조건으로는 50$^{\circ}C$에서 60% methanol를 용매로 사용했을 때 15분의 추출시간인 것으로 나왔으며 용매와 시료의 비율이 0.8 mL/g일 때 추출 수율이 가장 좋았다. 초음파를 60분 동안 처리 시 대조군에 비하여 2.06배의 추출 증가 효과를 볼 수 있었으며 microwave는 60초 조사 시에 최대의 추출 증가 효과를 나타내어 microwave를 처리하지 않은 시료와 비교하여 quercetin과 배당체가 2.14배 많이 추출되었다. 효소 처리 시 cellulase 용액과 시료의 비율이 0.5 mL/g일 때 quercetin과 배당체의 수율이 가장 높았으며 1.65 배 더 많이 추출되었고 viscozyme과 시료의 비율이 0.5 mL/g일 때 quercetin과 배당체의 수율이 가장 높았으며 추출증가율은 2.29 배인 것으로 나왔다. 가장 많은 quercetin과 배당체의 추출 수율을 보인 방법은 viscozyme과 시료의 비율이 0.5 mL/g일 때의 0.070${\pm}$0.002 mg/g인 것으로 나타났다.

Keywords

Acknowledgement

Supported by : 농림부

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