Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Water Extract of Liriope platyphylla on the mRNA Expression and Protein Secretion of Nerve Growth Factors

맥문동 유수추출물이 NGF의 mRNA발현과 단백질 분비에 미치는 영향

  • Choi, Sun-Il (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Park, Jee-Hee (Wellbeing Products Center, Pusan National University) ;
  • Her, Youn-Kyng (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Lee, Yoen-Kyung (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Kim, Ji-Eun (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Nam, So-Hee (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Goo, Jun-Seo (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Jang, Min-Ju (Wellbeing Products Center, Pusan National University) ;
  • Lee, Hee-Sup (Wellbeing Products Center, Pusan National University) ;
  • Son, Hong-Ju (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Lee, Chung-Yeol (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center) ;
  • Hwang, Dae-Youn (College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center)
  • 최선일 (부산대학교 생명자원과학대학) ;
  • 박지희 (부산대학교 밀양생물자원웰빙제품RIS사업단) ;
  • 허윤경 (부산대학교 생명자원과학대학) ;
  • 이연경 (부산대학교 생명자원과학대학) ;
  • 김지은 (부산대학교 생명자원과학대학) ;
  • 남소희 (부산대학교 생명자원과학대학) ;
  • 구준서 (부산대학교 생명자원과학대학) ;
  • 장민주 (부산대학교 밀양생물자원웰빙제품RIS사업단) ;
  • 이희섭 (부산대학교 밀양생물자원웰빙제품RIS사업단) ;
  • 손홍주 (부산대학교 생명자원과학대학) ;
  • 이충열 (부산대학교 생명자원과학대학) ;
  • 황대연 (부산대학교 생명자원과학대학)
  • Received : 2010.07.23
  • Accepted : 2010.10.06
  • Published : 2010.10.30
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Abstract

Liriope platyphylla has been though as an useful medical plant to improve the cough, sputum, neurodegenerative disorders, obesity, and diabetes in Korea and China from old times. In order to investigate the effects of Liriope platyphylla on expression and secretion of nerve growth factor (NGF), the mRNA expression and protein secretion were detected in the neuronal cell (B35) and neuroglial cell (C6) cultured with three differences concentration (5%, 10%, 15%) of Liriope platyphylla. In MTT assay and FACS anslysis, the some death of some B35 and C6 cells were observed in 15% extract-treated group, while other groups did not induce the death. Also, the mRNA expression of NGF were significantly increased in 5% and 10% extracts treated-group. Furthermore, the NGF protein concentration in supernatant collected from cultured cells showed the very similar pattern with mRNA expression. In order to verify the activity of secreted NGF, the culture supernatant collected from B35 and C6 cells cultured with Liriope platyphylla extracts for 24 hrs were treated into undifferentiated PC12 cells, and the differentiation level of PC12 cell were also observed with microscopes. The differentiation level of PC12 cell were significantly increased depend on the dose of extract. Therefore, these results suggested that the water extracts of Liriope platyphylla may contribute the regulation of NGF expression and secretion in the neuronal cell and be considered as an excellent candidate for a neurodegenerative disease-therapeutic drug.

Keywords

References

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