Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Pome Fruit Viruses on Pear Trees (Pyrus pyrifolia) in Korea

국내에서 발생하는 배나무 바이러스병

  • Cho, In-Sook (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dae-Huyn (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun-Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Chung, Bong-Nam (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Jeom-Doeg (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Gug-Seoun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 김대현 (농촌진흥청 국립원예특작과학원) ;
  • 김현란 (농촌진흥청 국립원예특작과학원) ;
  • 정봉남 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조점덕 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2010.11.05
  • Accepted : 2010.11.12
  • Published : 2010.12.01
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Abstract

Three pome fruit viruses, Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASPV) and Apple stem pitting virus (ASGV) were detected in pear trees (Pyrus pyrifolia) using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in Ansung, Naju and Ulsan provinces of Korea. Infection rate of three viruses was 35.2% from 452 leaf samples of the three cultivars of pear trees. Also, each of three viruses was detected by reverse transcription polymerase chain reaction (RT-PCR) for a limited number of samples. Infection rate of three viruses was 86.3% from 233 leaf samples of the three pear cultivars. The virus infection rates by RT-PCR were much higher than ELISA. ASGV was prevailing on pear with 74.2%, whereas ASPV and ACLSV were found in 34.8% and 0.4% of tested samples, respectively. Symptoms caused by ASGV showed black spots of infected Niitaka cultivar leaves. The ACLSV, ASPV and ASGV isolates showed 83~94% sequence identity at a nucleotide level to other pome fruit virus isolates when analyzed by NCBI BLAST. Pome fruit viruses occurring in pear were ACLSV, ASPV and ASGV. This is the first report of pear trees infected ASPV in Korea.

2009년 가을, 배 주산단지인 나주, 울산, 안성의 3개 지역에서 신고, 원황, 추황 품종을 대상으로 배 바이러스병 발생률을 ELISA와 RT-PCR로 조사한 결과 ELISA 검정은 ACLSV, ASPV, ASGV 3종 바이러스가 검출되어 35.2%의 발생률을 보였다. RT-PCR 검정은 ELISA 검정 보다는 적은 시료로 검정을 수행하였지만 ACLSV, ASPV, ASGV 3종 바이러스가 검출되었고 86.3%의 높은 발생률을 보였다. ELISA와 RT-PCR 진단 모두 ASGV > ASPV > ACLSV 순으로 ASGV의 발생률이 높게 나타났지만 발생률에는 ELISA 22.1%, RT-PCR 74.2%로 큰 차이가 있었다. 따라서 배 바이러스 검정은 ELISA 방법 보다 RT-PCR 방법이 바이러스 검출에 적합하다. ASGV가 감염된 신고 잎에서는 부정형의 검은점 증상이 보였으며 ASPV와 ACLSV가 감염된 식물체에서는 별다른 이상증상은 보이지 않았다. RT-PCR로 증폭된 ACLSV, ASPV, ASGV 유전자는 기존에 보고된 바이러스와 83~94%의 상동을 보였고, 특히, ASPV는 국내에서 처음으로 발생이 확인되었다.

Keywords

References

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