The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Diversity and Phylogenetic Relationship in Korean Strains of Lentinus lepideus Based on PCR Polymorphism

PCR 다형성 분석에 의한 한국산 잣버섯의 유전적 다양성 및 유연관계

  • Lee, Jae-Seong (Division of Life Sciences, University of Incheon) ;
  • Cho, Hae-Jin (Division of Life Sciences, University of Incheon) ;
  • Yoon, Ki-Nam (Division of Life Sciences, University of Incheon) ;
  • Alam, Nuhu (Division of Life Sciences, University of Incheon) ;
  • Lee, Kyung-Lim (Division of Life Sciences, University of Incheon) ;
  • Shim, Mi-Ja (Department of Life Science, University of Seoul) ;
  • Lee, Min-Woong (Department of Life Science, Dongguk University) ;
  • Lee, Yun-Hae (Mushroom Research Institute, Geonggi Agricultural Research and & Extension Service) ;
  • Jang, Myoung-Jun (Mushroom Research Institute, Geonggi Agricultural Research and & Extension Service) ;
  • Ju, Young-Chul (Mushroom Research Institute, Geonggi Agricultural Research and & Extension Service) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Pyung-Gyun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, U-Youn (Division of Life Sciences, University of Incheon) ;
  • Lee, Tae-Soo (Division of Life Sciences, University of Incheon)
  • 이재성 (인천대학교 생명과학부) ;
  • 조해진 (인천대학교 생명과학부) ;
  • 윤기남 (인천대학교 생명과학부) ;
  • ;
  • 이경림 (인천대학교 생명과학부) ;
  • 심미자 (서울시립대학교 생명과학과) ;
  • 이민웅 (동국대학교 생명과학과) ;
  • 이윤혜 (경기도 농업기술원 버섯연구소) ;
  • 장명준 (경기도 농업기술원 버섯연구소) ;
  • 주영철 (경기도 농업기술원 버섯연구소) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 이우윤 (인천대학교 생명과학부) ;
  • 이태수 (인천대학교 생명과학부)
  • Received : 2010.12.13
  • Accepted : 2010.12.21
  • Published : 2010.12.31
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Abstract

Lentinus lepideus, known as train wrecker fungus, has been used for nutritional and medicinal purposes. Recently, commercial cultivation technique and a new cultivar of the mushroom were developed. To investigate the genetic diversity and phylogenetic relationship for identifying the mushroom strains and cultivar, one commercial and 13 strains of Lentinus lepideus from different geographical regions of Korea were analyzed by ITS regions of rDNA and RAPD of genomic DNA. Three strains of Lentinus edodes were also used for the analysis. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 173 to 179 bp and 203 to 205 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical with 156 base pairs. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into four clusters, while 3 strains of L. edodes was divided into a new cluster. Ten primers out of 20 arbitrary primers used in the RAPD-PCR efficiently amplified the genomic DNA. The numbers of amplified DNA bands varied with the primers and strains, with polymorphic DNA fragments in the range from 0.2 to 2.6 kb. The results showed that phylogenetic relationship among Korean strains of Lentnus lepideus is high, but genetic diversity is low.

잣버섯(Lentinus lepideus)은 외국에서 철도의 침목을 부후시켜 기차가 탈선되는 원인을 제공하는 곰팡이로 알려져 있으나 예로부터 맛과 영양이 좋고 약리효과도 높아서 야생에서 채취하여 이용해왔다. 최근 이 버섯의 새로운 품종과 인공 재배법이 개발되면서 한국산 잣버섯의 유전적 다양성과 유연관계의 규명이 필요하게 되었다. 이에 우리나라의 여러 지역에서 수집한 14개의 잣버섯 균주와 3개의 표고균주를 대상으로 rDNA의 ITS region 염기서열과 genomic DNA의 RAPD-PCR을 수행하였다. ITS1과 ITS2 영역의 염기의 수는 각각 173에서 179와 203에서 205 염기쌍으로 계통에 따라 변이가 있었는데 ITS1 영역의 염기서열이 ITS2의 영역보다변이가 많았고 5.8S 지역은 염기의 수가 156쌍으로 모든 균주가 동일하였다. 각각의 균주 간 유연관계를 알아보기 위해 ITS 영역의 염기서열을 이용하여 분지도를 작성해 본 결과 실험에 사용한 균주는 4개의 클러스터로 나눠지는 것으로 나타났고 실험에 사용한 3개의 표고는 잣버섯과는 다른 새로운 클러스터로 나눠졌다. 또한 20 종류의 primer를 이용하여 RAPD를 수행한 결과 10개의 primer가 효과적으로 염색체 DNA를 증폭하는 것으로 나타났다. 증폭의 양상은 잣버섯의 계통과 primer의 종류에 따라 변이가 있었는데, 증폭된 DNA의 단편 수는 적은 것은 5개에서 많은 것은 37개, 단편의 크기는 0.2 kb에서 2.6 kb까지 다양하였다. 본 실험 결과 실험에 사용한 한국산 잣버섯의 계통과 품종간의 유연관계는 높았으나 유전적 다양성은 낮았다.

Keywords

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