The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Physicochemical Properties and Physiological Activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea

장수버섯 균사체가 배양된 옻피의 이화학적 특성 및 생리활성

  • Choi, Han-Seok (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Bo-Hyun (Department of Food Science and Technology, Chonbuk National University) ;
  • Yeo, Soo-Hwan (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA) ;
  • Jeong, Seok-Tae (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Ji-Ho (Fermentation and Food Processing Division, National Academy of Agricultural Science, RDA) ;
  • Park, Hyo-Suk (Department of Agricultural Chemistry, Wonkwang University) ;
  • Kim, Myung-Kon (Department of Bio Food Technology, Chonbuk National University)
  • 최한석 (농촌진흥청 국립농업과학원 발효이용과) ;
  • 김보현 (전북대학교 식품공학과) ;
  • 여수환 (농촌진흥청 국립농업과학원 발효이용과) ;
  • 정석태 (농촌진흥청 국립농업과학원 발효이용과) ;
  • 최지호 (농촌진흥청 국립농업과학원 발효이용과) ;
  • 박효숙 (원광대학교 농화학과) ;
  • 김명곤 (전북대학교 바이오식품공학과)
  • Received : 2010.11.12
  • Accepted : 2010.12.10
  • Published : 2010.12.31
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Abstract

The contents of proximate composition, free amino acids and phenolic acids in the Fomitella fraxinea cultivated-Rhus verniciflua stem bark(FRVSB), and its adipogenesis effect were investigated. The proximate composition(%) of FRVSB was as follows: moisture(7.64), ash(6.30), crude fat(3.86), crude protein(3.59) and sugar(not detected); while Rhus verniciflua stem bark(RVSB) contained 1.64, 8.09, 7.28, 6.48 and 5.39, respectively. The total free amino acids concentration was 97.41 mg% in FRVSB and 71.91 mg% in RVSB. Phosphoserine(55.06 mg%), ammonia(17.84mg%) and aspartic acid(6.05mg%) were predominant amino acids. The content of total phenolic acids was 422.89 ppm in ethanol extract and 283.86 ppm in water extract, with syringic and gallic acid as the main component. The FRVSB extracts showed a potent free radical scavenging activity for DPPH(2,2-diphenyl-1-picrylhydrazyl hydrate) with $IC_{50}$ of $28.54\;{\mu}g$(EtOH) and $54.70\;{\mu}g$(water), respectively, whereas $IC_{50}$ value of gallic acid was $1.84\;{\mu}g$. The protective effect of both ethanol and water extract the extracts against UV-induced oxidative stress in NIH3T3 was observed. The water extracts of FRVSB may promote adipogenesis in 3T3-L1 cells.

옻피의 일반성분은 수분 1.64%, 지방 8.09%, 단백질 7.28%, 회분 6.48%, 당류 5.39%로 구성되어 있었던 반면, 장수버섯을 증식시킨 옻나무 껍질은 각각 7.64%, 3.86%, 3.59%, 6.30%, 불검출 이었다. 배양물의 총 유리아미노산 함량은 97.41mg%으로 대조구(71.91 mg%)에 비하여 35% 증가하였으며 phosphoserine(55.06 mg%), ammonia(17.84 mg%), aspartic acid (6.05 mg%)가 주요 유리아미노산 이었다. 배양물의 총 phenolic acid함량은 422.89 ppm/283.86 ppm(알코올/물 추출물)이었으며 syringic acid와 gallic acid가 주요 성분이었고 vanillic, protocatechuic acid가 소량 함유되어 있었다. 항산화활성(DPPH, $IC_{50}$)은 $28.54\;{\mu}g/54.70\;{\mu}g$ (알코올/물 추출물)으로 gallic acid가($IC_{50}\;1.84\;{\mu}g$) 주요 영향 요소이었다. 배양물의 NIH3T3세포에 세포독성($IC_{50}$)은 알코올 추출물 $50\;{\mu}g/200\;{\mu}L$, 물 추출물 $90\;{\mu}g/200\;{\mu}L$이었으며 UV조사에 의해 유도된 산화적 stress에 대하여 $20-25\;{\mu}g/200\;{\mu}L$ 농도에서 항산화 활성을 보였다. 배양물의 물 추출은 전구지방세포(3T3-L1)의 분화를 촉진시켰으며 $10\;{\mu}g/200\;{\mu}L$의 농도에서 가장높았다.

Keywords

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