Tissue Engineering and Regenerative Medicine

Korean Tissue Engineering and Regenerative Medicine Society (한국조직공학·재생의학회)
권호 표지

Characterization of Stem Cells Obtained from the Dental Pulp and Periodontal Ligament of Deciduous Teeth

유치의 치수와 치주인대에서 분리한 줄기세포의 특성

  • Song, Je-Seon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Kim, Seung-Hye (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Kim, Seong-Oh (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Choi, Byung-Jai (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Yonsei University) ;
  • Kim, Jeong-Hee (Department of Oral Biochemistry, Institute of Oral Biology, College of Dentistry, Kyung Hee University) ;
  • Kwak, Sung-Wook (Division in Anatomy & Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University) ;
  • Jung, Han-Sung (Division in Anatomy & Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University)
  • 송제선 (연세대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 김승혜 (연세대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 김성오 (연세대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 최병재 (연세대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 김정희 (경희대학교 치과대학 구강생화학교실 및 구강생물연구소) ;
  • 곽성욱 (연세대학교 치과대학 구강생물학교실 내 해부발생학 연구실 및 구강악안면 경조직 재생연구소) ;
  • 정한성 (연세대학교 치과대학 구강생물학교실 내 해부발생학 연구실 및 구강악안면 경조직 재생연구소)
  • Published : 2010.12.01
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Abstract

Many studies have found adult stem cells in human teeth or correlated tissues. However, most of these stem cells were found in the permanent teeth or pulp from exfoliated deciduous teeth. The aim of the present study was to characterize stem cells isolated from the functional dental pulp and periodontal ligament of deciduous teeth. Dental pulp tissue was obtained from deciduous teeth by extirpation during treatment for dental caries, and periodontal tissue was obtained from deciduous teeth that were extracted for orthodontic reasons or space management. We observed cell outgrowth from these tissues in 'explants culture', and named these cells as deciduous dental pulp stem cells (DDPSCs) and deciduous periodontal ligament stem cells (DPDLSCs), respectively. These stem cells presented embryonic stem cell markers (Oct-4 and Nanog), an ectomesenchymal stem cell marker (Nestin), and mesenchymal stem cell markers (Stro-1 and CD146). In differentiation media, DDPSCs and DPDLSCs were able to change into cells that produce lipid vacuoles or cells that induce extracellular mineral aggregation, expressing genes correlated with adipogenesis ($PPAR\gamma2$ and LPL) or osteogenesis (ALP and BSP), respectively. This is the first report of the presence of multipotent stem cells in the functional dental pulp and periodontal ligament tissue of deciduous teeth, which can be isolated using an explants culture method. These tissues can be obtained easily during routine dental procedures, and could therefore represent a good source of adult stem cells for use in regenerative medicine.

Keywords

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