Journal of Korean Medical Science

Korean Acad Medical Sciences (대한의학회)
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Polyphenol (-)-Epigallocatechin Gallate during Ischemia Limits Infarct Size Via Mitochondrial $K_{ATP}$ Channel Activation in Isolated Rat Hearts

  • Song, Dae-Kyu (Department of Physiology, School of Medicine, Keimyung University) ;
  • Jang, Young-Ho (Department of Anesthesiology, Pureun Hospital) ;
  • Kim, June-Hong (Institute of Cardiovascular Research, Pusan National University Yangsan Hospital) ;
  • Chun, Kook-Jin (Institute of Cardiovascular Research, Pusan National University Yangsan Hospital) ;
  • Lee, Deok-Hee (Department of Anesthesiology and Pain Medicine, College of Medicine, Yeungnam University) ;
  • Xu, Zhelong (Department of Anesthesiology, University of North Carolina)
  • Published : 2010.03.02
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Abstract

Polyphenol (-)-epigallocatechin gallate (EGCG), the most abundant catechin of green tea, appears to attenuate myocardial ischemia/reperfusion injury. We investigated the involvement of ATP-sensitive potassium ($K_{ATP}$) channels in EGCG-induced cardioprotection. Isolated rat hearts were subjected to 30 min of regional ischemia and 2 hr of reperfusion. EGCG was perfused for 40 min, from 10 min before to the end of index ischemia. A nonselective $K_{ATP}$ channel blocker glibenclamide (GLI) and a selective mitochondrial $K_{ATP}$ ($mK_{ATP}$) channel blocker 5-hydroxydecanoate (HD) were perfused in EGCG-treated hearts. There were no differences in coronary flow and cardiodynamics including heart rate, left ventricular developed pressure, rate-pressure product, +dP/$dt_{max}$, and -dP/$dt_{min}$ throughout the experiments among groups. EGCG-treatment significantly reduced myocardial infarction ($14.5{\pm}2.5%$ in EGCG $1{\mu}M$ and $4.0{\pm}1.7%$ in EGCG $10{\mu}M$, P<0.001 vs. control $27.2{\pm}1.4%$). This antiinfarct effect was totally abrogated by $10{\mu}M$ GLI ($24.6{\pm}1.5%$, P<0.001 vs. EGCG). Similarly, $100{\mu}M$ HD also aborted the anti-infarct effect of EGCG ($24.1{\pm}1.2%$, P<0.001 vs. EGCG ). These data support a role for the $K_{ATP}$ channels in EGCG-induced cardioprotection. The $mK_{ATP}$ channels play a crucial role in the cardioprotection by EGCG.

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References

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