Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The Effect of Stocking Density on Stress Related Genes and Telomeric Length in Broiler Chickens

  • Beloor, J. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Kang, H.K. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Kim, Y.J. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Subramani, V.K. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Jang, I.S. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Sohn, S.H. (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Moon, Yang Soo (Department of Animal Science & Biotechnology, Jinju National University)
  • Received : 2009.07.27
  • Accepted : 2009.10.05
  • Published : 2010.04.01
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Abstract

To be economically profitable, the poultry industry demands an increase in stocking density, which could adversely affect chicken welfare. The current study was performed to investigate the effect of stocking density on stress-related, heat shock protein genes (HSP70 and HSP90), 3-hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) gene and telomere length in broiler chickens. Seven-day-old broiler chickens were housed at High (0.0578 $m^2$/bird), Standard (0.077 $m^2$/bird) and Low (0.116 $m^2$/bird) stocking densities with 8 replicates each until 35 d of age. The growth performance, such as body weight gain and average daily feed intake, was found to be significantly (p<0.05) higher in the Low density group, but these parameters did not show any difference between the High and Standard groups. Other growth performance, such as feed conversion ratio and final feed intake, showed no difference among the treated groups. The expression levels of HSP70 and HMGCR were found to be elevated with the increase of stocking density. The expression level of these genes was significantly (p<0.05) higher in the High density stocked group compared with the other groups, whereas the expression levels were not significantly different between the Low and Standard groups. The expression levels of HSP90 did not show any significant changes among the treated groups. The telomeric length of the birds housed in High density was reduced significantly (p<0.05) when compared to that of the birds in Low density. These results clearly indicate that birds stocked at high density show physiological adaptive changes indicative of stress at gene transcriptional and telomere levels.

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References

  1. Basu, N. and A. E. Todgham. 2002. Heat shock protein genes and their functional significance in fish. Gene 295(2):173-183 https://doi.org/10.1016/S0378-1119(02)00687-X
  2. Bilgili, S. F. and J. B. Hess. 1995. Placement density influences broiler carcass grade and meat yields. J. Appl. Poult. Res. 4:384-389
  3. Bolton, W., R. Thompson, R. M. Jones and W. A. Dewar. 1972. Effect of stocking density on performance of broiler chicks. Br. Poult. Sci. 13:157-162 https://doi.org/10.1080/00071667208415929
  4. Cravener, T. L., W. B. Roush and M. M. Mashaly. 1992. Broiler production under varying population-densities. Poult. Sci. 71:427-433 https://doi.org/10.3382/ps.0710427
  5. Dozier, W. A., J. P. Thaxton, S. L. Branton, G. W. Morgan, D. M. Miles, W. B. Roush, B. D. Lott and Y. Vizzier-Thaxton. 2005b. Stocking density effects on growth performance and processing yields of heavy broilers. Poult. Sci. 84:1332-1338
  6. Ekstrand, C., B. Algers and J. Svedberg. 1997. Rearing conditions and foot-pad dermatitis in Swedish broiler chickens. Prev. Vet. Med. 31:167-174 https://doi.org/10.1016/S0167-5877(96)01145-2
  7. Estevez, I., R. C. Newberry and L. Arias de Reyna. 1997. Broiler chickens: A tolerant social system? Etologia 5:19-29
  8. Estevez, I. 2007. Density allowances for broilers: where to set the limits? Poult. Sci. 86:1265-1272
  9. Feddes, J. J. R., E. J. Emmanuel and M. J. Zuidhof. 2002. Broiler performance, BW variance, feed and water intake, and carcass quality at different stocking densities. Poult. Sci. 81:774-779
  10. Gornati, R., E. Papis, R. Simona, T. Genciana, S. Marco and B. Giovanni. 2004. Rearing density influences the expression of stress-related genes in sea bass (Dicentrarchus labrax, L.). Gene 341:111-118 https://doi.org/10.1016/j.gene.2004.06.020
  11. Heckert, R. A., I. Estevez, E. Russek-Cohen and R. Pettit-Riley. 2002. Effects of density and perch availability on the immune status of broilers. Poult. Sci. 81:451-457
  12. Heishman, J. O., C. O. Cunningham and T. B. Clark. 1952. Floor space requirement of broilers. Poult. Sci. 31:920
  13. Hermesz, E., A. Magdolna and J. Nemcsok. 2001. Identification of two hsp90 genes in carp. Comp. Biochem. Physiol. Part C 129: 397-407 https://doi.org/10.1016/S1532-0456(01)00216-2
  14. Hong, Y. H., Y. Nishimura, D. Hishikawa, H. Tsuzuki, H. Miyahara, C. Gotoh, K. C. Choi, D. D. Feng, C. Chen, H. G. Lee, K. Katoh, S. G. Roh and S. Sasaki. 2005. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology 146:5092-5099 https://doi.org/10.1210/en.2005-0545
  15. Houben, M. J., J. J. Moonen, J. van Schooten and J. Hageman. 2007. Telomere length assessment: Biomarker of chronic oxidative stress? Free Radic. Biol. Med. 44:235-246 https://doi.org/10.1016/j.freeradbiomed.2007.10.001
  16. Martrenchar, A., J. P. Morisse, D. Huonnic and J. P. Cotte. 1997. Influence of stocking density on some behavioural, physiological, and productivity traits of broilers. Vet. Res. 28:473-480
  17. Martrenchar, A., D. Huonnic, J. P. Cotte, E. Boilletot and J. P. Morisse. 2000. Influence of stocking density, artificial dusk and group size on the perching behaviour of broilers. Br. Poult. Sci. 41:125-130 https://doi.org/10.1080/713654921
  18. Pettit-Riley, R. and I. Estevez. 2001. Effects of density on perching behavior of broiler chickens. Appl. Anim. Behav. Sci. 71:127-140 https://doi.org/10.1016/S0168-1591(00)00174-X
  19. Proudfoot, F. G., H. W. Hulan and D. R. Ramey. 1979. Effect of 4 stocking densities on broiler carcass grade, the incidence of breast blisters, and other performance traits. Poult. Sci. 58:791-793 https://doi.org/10.3382/ps.0580791
  20. Sanotra, G. S., L. G. Lawson and K. S. Vestergaard. 2001. Influence of stocking density on tonic immobility, lameness, and tibial dyschondroplasia in broilers. J. Appl. Anim. Welf. Sci. 4:71-87 https://doi.org/10.1207/S15327604JAWS0401_4
  21. Schlesinger, J. M. 1986. Heat shock proteins. J. Cell Biol. 103:321-325 https://doi.org/10.1083/jcb.103.2.321
  22. Shanawany, M. M. 1988. Broiler performance under high stocking densities. Br. Poult. Sci. 29:43-52 https://doi.org/10.1080/00071668808417025
  23. Sohn, S. H., E. J. Cho., W. J. Son and C. Y. Lee. 2007. Diagnosis of bovine freemartinism by fluorescence in situ hybridization on interphase nuclei using a bovine Y chromosome specific DNA probe. Theriogenology 68:1003-1011 https://doi.org/10.1016/j.theriogenology.2007.06.022
  24. Tomhave, A. E. and K. C. Seeger. 1945. Floor space requirements of broilers. Delaware Agric. Exp. Sta. Bull. 255
  25. Turkyilmaz, M. K. 2006. The effect of stocking density on stress reaction in broiler chickens during summer Turk. J. Vet. Anim. Sci. (1):31-36
  26. Von Zglinicki, T., G. Saretzki, W. Docke and C. Lotze. 1995. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: a model for senescence? Exp. Cell Res. 220:186-193 https://doi.org/10.1006/excr.1995.1305
  27. Von Zglinicki, T. 2002. Oxidative stress shortens telomeres. Trends Biochem. Sci. 27:339-344 https://doi.org/10.1016/S0968-0004(02)02110-2
  28. Zager, R. A. and A. Johnson. 2001. Renal cortical cholesterol accumulation is integral component of the systemic stress response. Kidney Int. 60:2299-2310 https://doi.org/10.1046/j.1523-1755.2001.00071.x
  29. Zulkifli, I. and A. Sti Nor Azah. 2004. Fear and stress reactions, and the performance of commercial broiler chickens subjected to regular pleasant and unpleasant contacts with human being. Appl. Anim. Behav. Sci. 88:77-87 https://doi.org/10.1016/j.applanim.2004.02.014

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