Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Optimal Extraction Conditions of Anti-obesity Lipase Inhibitor from Phellinus linteus and Nutritional Characteristics of the Extracts

  • Lee, Jong-Kug (Chungnam Agricultural Research and Extension Services) ;
  • Song, Jung-Hwa (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In an effort to develop novel mushroom-derived anti-obesity nutraceuticals, water and ethanol extracts containing the lipaseinhibitory compound from Phellinus linteus were prepared, and their nutritional components were determined. The optimal conditions for the extraction of P. linteus lipase inhibitor involved the treatment of the fruiting bodies with distilled water at $80^{\circ}C$ for 72 hr and 80% ethanol at $100^{\circ}C$ for 60 hr, respectively. The distilled water extract and ethanol extract contained 10.9% and 6.11% of crude protein, and 0.96% and 15.86% of crude fat, respectively. Additionally, the distilled water extract contained a large quantity of minerals, including 239.5 mg of K, 39.3 mg of Mg, and 39.3 mg of Na. The free amino acid content of the distilled water extracts was also higher than that of the ethanol extracts, and in particular, the distilled water extracts contained 5,139 mg of asparagine, 3,891 mg of tryptophan, 2,598 mg of alanine, and 2,066 mg of serine in 100 g of the distilled water extracts. 100 g of the distilled water and ethanol extracts were found to contain 12.31 g and 8.16 g of malic acid, respectively.

Keywords

References

  1. Lee JH, Cho SM, Kim HM, Hong ND, Yoo ID. Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions. J Microbiol Biotechnol 1997;7:52-5.
  2. Lim JS, Kim SH, Park JS, Choi JY, Park SJ, Shin KS. Antitumor activity of the fruitbody extract of Basidiomycete, Phellius linteus. Kor J Microbiol 2001;39:121-5.
  3. Park IH, Jeon SY, Lee HJ, Kim SI, Song KS. A beta-secre-tase (BACE1) inhibitor hispidin from the mycelial cultures of Phellinus linteus. Planta Med 2004;70:143-6. https://doi.org/10.1055/s-2004-815491
  4. Shon YH, Lee JS, Lee HW, Kim JW, Lim JK, Kim CH, et al. Antimutagenicity of Phellinus linteus in Salmonella typhimurium. J Microbiol 1999;37:136-40.
  5. Lee JK, Jang JH, Lee JT, Lee JS. Extraction and characteristics of anti-obesity lipase inhibitor from Phellinus linteus. Mycobiology 2010 (in press)
  6. Bray GA, Blackburn GL, Ferguson JM, Greenway FL, Jain AK, Mendel CM, et al. Sibutramine produces dose-related weight loss. Obes Res 1999;7:189-98. https://doi.org/10.1002/j.1550-8528.1999.tb00701.x
  7. Seo DS, Jang JH, Kim NM, Lee JS. Optimal extraction condition and characterization of antidementia acetylcholinesterase inhibitor from Job’s tear (Coix lachrymajobi L.). Korean J Medicinal Crop Sci 2009;17:434-8.
  8. Bitou N, Ninomiya M, Tsujita T, Okuda H. Screening of lipase inhibitors from marine algae. Lipids 1999;34:441-5. https://doi.org/10.1007/s11745-999-0383-7
  9. Kim SG, An GH, Yoon SW, Lee YC, Ha SD. A study on dietary supplement to reduce obesity by the mechanism of decreasing lipid and carbohydrate absorption. Korean J Food Sci Technol 2003;35:519-26.
  10. Yoshizmi K, Hirano H, Ando H, Hirai Y, Ida Y, Tsuji T, et al. Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J Agric Food Chem 2006;54: 335-41. https://doi.org/10.1021/jf052047f
  11. Yang HK, Kim YS, Bae HS, Cho KH, Shin JE, Kim NJ, et al. Rhei rhizoma and chunghyuldan inhibit pancreatic lipase. Nat Product Sci 2003;9:38-43.
  12. Kim JH, Kim HJ, Kim C, Jung H, Kim YO, Ju JY, et al. Development of lipase inhibitors from various derivatives of monascus pigment produced by Monascus fermentation. Food Chem 2007;101:357-64. https://doi.org/10.1016/j.foodchem.2005.11.055
  13. Bray GA, Greenway FL. Current and potential drugs for treatment of obesity. Endocr Rev 1999;20:805-75. https://doi.org/10.1210/er.20.6.805
  14. Zapf J, Schoenle E, Waldvogel M, Sand I, Froesch ER. Effect of trypsin treatment of rat adipocytes on biological effects and binding of insulin and insulin-like growth factors: further evidence for the action of insulin-like growth factors through the insulin receptor. Eur J Biochem 1981; 133:605-9.
  15. Helrich K. Official methods analysis of the Association of the Official Analytical Chemists. chapter 3.1.01-05. 15th ed. Washington, DC: Association of the Official Analytical Chemists; 1990.
  16. Lee JS, Min GH, Lee JS. Nutritional and physicochemical characteristics of the antidementia acetylcholinesterase-inhibiting methanol extracts from Umbilicaria esculenta. Mycobiology 2009;37:203-6. https://doi.org/10.4489/MYCO.2009.37.3.203
  17. Jeong CH, Kang ST, Joo OS, Lee SC, Shin YH, Shin KH, et al. Phenolic content, antioxidant effect and acetylcholinesterase inhibitory activity of Korean commercial green, puer, oolong and black teas. Korean J Food Preserv 2009;16:230-7.
  18. Kwak JH, Jeong CH, Kim JH, Choi GN, Shin YH, Cho SH, et al. Acetylcholinesterase inhibitory effect of green tea extracts according to storage condition. Korean J Food Sci Technol 2009;41:435-40.

Cited by

  1. Antioxidant property and α $$ \boldsymbol{\upalpha} $$ -glucosidase, α $$ \boldsymbol{\upalpha} $$ -amylase and lipase inhibiting activities of Flacourtia inermis fruits: characterization of malic acid as an inhibitor of the enzymes vol.52, pp.12, 2015, https://doi.org/10.1007/s13197-015-1937-6
  2. Medicinal mushroom: boon for therapeutic applications vol.8, pp.8, 2018, https://doi.org/10.1007/s13205-018-1358-0
  3. Antiobesity and Antioxidant Potentials of Selected Palestinian Medicinal Plants vol.2018, pp.1741-4288, 2018, https://doi.org/10.1155/2018/8426752