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The Korean Association of Immunobiologists (대한면역학회)
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Dendritic Cell Activation by Glucan Isolated from Umbilicaria Esculenta

  • Kim, Hyung-Sook (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University) ;
  • Kim, Jee-Youn (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University) ;
  • Lee, Hong-Kyung (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University) ;
  • Kim, Moo-Sung (Macrocare) ;
  • Lee, Sang-Rin (Macrocare) ;
  • Kang, Jong-Soon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hwan-Mook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Ae (Institute of Natural Sciences, Yong-In University) ;
  • Hong, Jin-Tae (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University) ;
  • Kim, Young-Soo (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University) ;
  • Han, Sang-Bae (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
  • Received : 2010.10.22
  • Accepted : 2010.11.08
  • Published : 2010.12.31
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Abstract

Background: Lichen-derived glucans have been known to stimulate the functions of immune cells. However, immunostimulatory activity of glucan obtained from edible lichen, Umbilicaria esculenta, has not been reported. Thus we evaluated the phenotype and functional maturation of dendritic cells (DCs) following treatment of extracted glucan (PUE). Methods: The phenotypic and functional maturation of PUE-treated DCs was assessed by flow cytometric analysis and cytokine production, respectively. PUE-treated DCs was also used for mixed leukocyte reaction to evaluate T cell-priming capacity. Finally we detected the activation of MAPK and NF-${\kappa}B$ by immunoblot. Results: Phenotypic maturation of DCs was shown by the elevated expressions of CD40, CD80, CD86, and MHC class I/II molecules. Functional activation of DCs was proved by increased cytokine production of IL-12, IL-$1{\beta}$, TNF-${\alpha}$, and IFN-${\alpha}/{\beta}$, decreased endocytosis, and enhanced proliferation of allogenic T cells. Polymyxin B, specific inhibitor of lipopolysaccharide (LPS), did not affect PUE activity, which suggested that PUE was free of LPS contamination. As a mechanism of action, PUE increased phosphorylation of ERK, JNK, and p38 MAPKs, and enhanced nuclear translocation of NF-${\kappa}B$ p50/p65 in DCs. Conclusion: These results indicate that PUE induced DC maturation via MAPK and NF-${\kappa}B$ signaling pathways.

Keywords

References

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