Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Cellular Protective Effect and Component Analysis of Euphorbia humifusa Extracts

땅빈대 추출물의 세포 보호 효과 및 성분 분석에 관한 연구

  • Kim, Sun-Young (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic) ;
  • Won, Doo-Hyun (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic) ;
  • Lim, Myoung-Sun (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic) ;
  • Park, Soo-Nam (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
  • 김선영 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터) ;
  • 원두현 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터) ;
  • 임명선 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터) ;
  • 박수남 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터)
  • Received : 2010.11.15
  • Accepted : 2010.12.16
  • Published : 2011.12.31
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Abstract

In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction ($3.68\;{\mu}g/mL$) and aglycone fraction ($3.15\;{\mu}g/mL$) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) of Euphorbia humifusa extract on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($0.43\;{\mu}g/mL$) and aglycone fraction ($0.35\;{\mu}g/mL$) of extract showed higher ROS scavenging activity than L-ascorbic acid ($1.50\;{\mu}g/mL$). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner ($5{\sim}25\;{\mu}g/mL$), and was more effective than (+)-${\alpha}$-tocopherol, lipid peroxidation chain blocker. Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.

Keywords

References

  1. Fantone, J. C. and Ward, P. A. (1982) Role of oxygenderived free radicals and metabolites in leukocyte dependent inflammatory reaction. Ann. J. Path. 107(3): 395-418.
  2. Jurkiewicz, B. A. and Buettner, G. R. (1994) Ultraviolet lightinduced free radial formation in skin : An electron paramagnetic resonance study. Photochem. Photobiol. 59(1): 1-4.
  3. Davies, K. J. A. (1987) Protein damage and degradation by oxygen radical. J. biol. Chem. 262(20): 9902-9907.
  4. Park, S. N. (1989) Dissertation, Seoul National Univ., Seoul, Korea.
  5. Jurkiewicz, B. A. and Buettner, G. R. (1994) Ultraviolet lightinduced free radical formation in skin: An electron paramagnetic resonance study. Photochem. Photobiol. 59: 1-4. https://doi.org/10.1111/j.1751-1097.1994.tb04993.x
  6. Packer, L. (1994) Ultraviolet radiation (UVA, UVB) and skin antioxidants. In: Free radical damage and its control. Rice-Evans, C. A. and Burdon, R. H. (eds.). 239-253. Elsevier Science, St. Louis.
  7. Jurkeiwicz, B. A., Bissett, D. L. and Buettner, G. R. (1995) Effect of topically applied tocopherol on ultraviolet radiationmediated free radical demage in skin. J. Invest. Dermatol. 104: 484-488. https://doi.org/10.1111/1523-1747.ep12605921
  8. Thomas, J. P. and Girotti, A. W. (1988) Photogenenration of singlet oxygen by membrane bound hematoporphyrin derivative. Photochem. Photobiol. 47: 79s.
  9. Zheng, P. and Kligman, L. H. (1992) UVA induced biochemical changes in hairless mouse skin : A comparison to UVB-Induces Damage. J. Invest. Dermatol. 100: 194-199
  10. Foote, C. S. (1991) Definition of type I and type II photosensitized oxidation. Photochem. Photobiol. 54: 659-666. https://doi.org/10.1111/j.1751-1097.1991.tb02071.x
  11. Darr, D. and Fridovich, I. (1994) Free radicals in cutameous biology. F. Invest. Dermatol. 102: 671-675. https://doi.org/10.1111/1523-1747.ep12374036
  12. Scharffetter-Kochanek, K. (1997) Photoaging of the connective tissue of skin: Its prevention and therapy, antioxidants in disease mechanism and therapy. Adv. Pharmacol. 38: 639-655.
  13. Cheseman, K. H. and Slater, T. F. (1993) Free radicals inmedicine. British Medical Bulletin 49(3): 566-576.
  14. McCord, J. M. and Fridovich, I. (1969) Superoxide dismutase an enzymic function for erythrocuprein. J. Biol. Chem. 244: 6049-6103.
  15. 배기환 (2000) 한국의 약용식물, 277. 교학사, 서울.
  16. Eeo, S. I., Hu, W., Han, W. and Wang, M. H. (2008) Antioxidant activity and cytotoxic effect of extracts from Euphorbia humifusa. Kor. J. Phamacogn. 39: 295-299.
  17. Cha, B. C. Lee, S. K. Lee, H. W. E. Choi, M. Y. Rhim, T. J. and Park, H. J. (1997) Antioxidative effect of domestic plants. Kor. J. Pharmacogn. 28(1): 15-20