Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anti-diabetic Effects of Triticum aestivum L. Water Extracts in db/db Mice as an Animal Model of Diabetes Mellitus Type II

제2형 당뇨모델 db/db 마우스에서 밀순 물추출물의 항당뇨 효과

  • Lee, Sun-Hee (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lim, Sung-Won (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Hur, Jung-Mu (Chong Kun Dang Healthcare Corp. Research Center) ;
  • Lee, Hoi-Seon (Faculty of Biotechnology and Center for Agricultural Science and Technology, College of Agriculture and Life Science, Chonbuk National University) ;
  • Kim, Dae-Ki (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School)
  • 이선희 (전북대학교 의과대학 및 의과학연구소) ;
  • 임성원 (전북대학교 의과대학 및 의과학연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과) ;
  • 허정무 (종근당 건강(주)) ;
  • 이회선 (전북대학교 농업생과학대학 응용생물공학부) ;
  • 김대기 (전북대학교 의과대학 및 의과학연구소)
  • Received : 2010.09.15
  • Accepted : 2010.10.28
  • Published : 2011.12.31
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Abstract

We evaluated the anti-diabetic effects of Triticum aestivum sprout water extract (TA) in diabetes mellitus type 2. For the experiments, the diabetic animal model db/db mice were divided to 3 groups: diabetic control (db/db) and two experimental groups orally treated with 25 and 100 mg/kg single dose of TA (TA-25 and TA-100, respectively). The lean mice were used as the non-diabetic normal control. All mice have free access to water and AIN-93 diet. TA was administrated to diabetic mice for 5 weeks and the diabetic clinical markers, including blood glucose level, body weight, food intake and insulin level, were measured at a time. After administration for 5 weeks, the blood glucose level was decreased 1.10 and 1.98 folds in TA-25 and TA-100 groups, respectively, compared with db/db group. The body weight and diet consumption were significantly reduced by TA treatment in dose-dependent manner. The treatments of TA-100 also significantly decreased remarkedly liver weight and slightly serum insulin levels when compared with them of the diabetic control group. However the immunohistochemical staining for insulin clearly showed high expression of insulin in the pancreatic islet cells derived from all db/db mice, even if TA was administrated. Moreover, TA-100 treatment significantly improved impaired glucose tolerance in diabetic db/db mice. The results suggest that TA has anti-hyperglycemic effect attenuating blood glucose in the animal model of type 2 diabetes and might be useful as a functional diet for human diabetic diseases.

Keywords

References

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