Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Medicinal Herb Extracts on Osteoblast Differentiation and Osteoclast Formation

한약재 추출물의 조골세포 분화 및 파골세포 형성에 미치는 영향

  • Im, Nam-Kyung (Department of Food and Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Kim, Mi-Jin (Department of Food and Technology, Keimyung University) ;
  • Lee, Eun-Ju (Department of Food and Technology, Keimyung University) ;
  • Kim, Hyuk-Il (Department of Food and Technology, Keimyung University) ;
  • Lee, In-Seon (Department of Food and Technology, Keimyung University)
  • 임남경 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통미생물자원개발 및 산업화 연구센터) ;
  • 김미진 (계명대학교 식품가공학과) ;
  • 이은주 (계명대학교 식품가공학과) ;
  • 김혁일 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 식품가공학과)
  • Received : 2010.02.20
  • Accepted : 2010.04.20
  • Published : 2010.10.31
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Abstract

Bone is continuously remodeled by osteoblasts and osteoclasts. We investigated the effects of medicinal herbs, which act on bone metabolism. Fifteen kinds of medicinal herb extracts were screened for bone formation activity with osteoblastic cells, and MC3T3-E1 and bone resorption were screened with osteoclasts derived from mouse bone marrow macrophages. Among these samples, Actinidia polygama, Eucommia ulmoides Oliv., Schizonepeta tenuifolia, Sorbus commixta, and Zingiber officinale Rosc. extracts showed strong bone-forming activity accompanied with osteoblast proliferation and alkaline phosphatase activity. In addition, these extracts decreased tartrate-resistant acid phosphatase activity against osteoclast differentiation. The results indicate that these medicinal herb extracts can potentially prevent bone-related diseases such as osteoporosis by increasing osteoblast differentiation and reducing osteoclast activity.

천연물 유래의 생약에서 조골세포 증식을 높이면서, 파골세포의 분화 억제에 효과가 있는 시료를 검색하고자 15종의 한약재추출물의 효과를 검토해 보았다. Mouse calvaria 유래의 osteoblastic cells를 이용하여 세포 생존률 및 ALP 활성을 측정하였으며, 또한 마우스 골수 세포를 이용하여 M-CSF와 RANKL을 처리하여 파골세포의 분화를 유도한 후, 세포 생존율과 TRAP효소활성을 측정하였다. 그 결과, 두충, 곽향, 개다래, 형개, 정공등 추출물은 조골세포 증식 및 ALP 활성를 증가시켰으며, 파골세포 활성을 나타내는 TRAP활성이 억제되는 것으로 확인되었다. 따라서 이 추출물들은 조골세포의 기능을 향상시키는 동시에 파골세포의 기능을 억제하여 골 흡수와 관련된 질환과 함께 골 질환 예방에 효과가 있을 것으로 생각되어진다.

Keywords

References

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