KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes

에탄올 발효방법에 따른 음식물류 폐기물의 바이오에탄올 생산성 비교

  • Kang, Hee-Jeong (Dep. of Environmental Engineering, Chonnam National University) ;
  • Li, Hong-Xian (Dep. of Environmental Engineering, Chonnam National University) ;
  • Kim, Yong-Jin (Dep. of Maritime Environmental Engineering, Mokpo National Maritime University) ;
  • Kim, Seong-Jun (Dep. of Environmental Engineering, Chonnam National University)
  • 강희정 (전남대학교 환경공학과) ;
  • 리홍선 (전남대학교 환경공학과) ;
  • 김용진 (목포해양대학교 해양환경공학) ;
  • 김성준 (전남대학교 환경공학과)
  • Received : 2010.09.24
  • Accepted : 2010.10.22
  • Published : 2010.10.31
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Abstract

In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 $h^{-1}$), 6 h (0.07 $h^{-1}$), 3 h (0.13 $h^{-1}$). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 $h^{-1}$.

Keywords

References

  1. Sheoran, A., B. S. Yadav, P. Nigam, and D. Singh (1998) Continuous ethanol production from sugarcane molasses using a column reactor of immobilized Saccharomyces cerevisiae HAU-1. J. Basic Microbiol. 38: 123-128. https://doi.org/10.1002/(SICI)1521-4028(199805)38:2<123::AID-JOBM123>3.0.CO;2-9
  2. Kim, N. Y., H. J. Kim, and J. S. Kim (2005) Simultaneous saccharification and fermentation from ammonia pretreated waste oak wood for fuel ethanol production. Master. Theories and Applications of Chem. Eng. 11: 1731-1734.
  3. Park, J. I., H. C. Woo, and J. H. Lee (2008) Production of bio-energy from marine algae: status and perspectives. Korean J. Chem. Eng. Res. 46: 833-844.
  4. Lee, S. M., J. H. Kim, H. Y. Cho, J. H. Lee (2009) Production of bio-ethanol from brown algae by physicochemical hydrolysis, Korean J. Ind. Eng. Chem. 20: 517-521.
  5. Kim, K. S. (2006) The present state of domestic acceptance of various international conventions for the prevention of marine pollution. Korean J. Society of Marine Environ & Safety 12: 293-300.
  6. Kim, S. H., T. K. Kwak, E. H. Choi, K. E. Lee (2007) Food waste management practices and influencing factors at elementary school food services. Korean J. Community. Nutrition 12: 815-825.
  7. Choi, B. S. (2002) A Study on the Policy that Makes the Foodwaste into the Resources. MS. Thesis. Paichai University, Daejeon, Korea.
  8. Han, H. J., H. X. Li, and S. J. Kim (2006) Ethanol production by synchronous saccharification and fermentation using food wastes. Korean J. Biotechnol. Bioeng. 21: 474-478.
  9. Pack, M. Y. (1975) Double-stage batch fermentation of beer 1. Theoretical background. Korean J. Applied. Microbiol. Bioeng. 3: 31-34.
  10. Park, S. H. and C. Y. Choi (1982) Optimal ethanol fermentation by batch and glucose fed-batch culture. Korean J. Ins. Chem. Eng. 20: 37-48.
  11. Hewitt, C. J. and A. W. Nienow (2007) The scale-up of microbial batch and fed-batch fermentation processes. Advances in applied microbiol. 62: 105-135. https://doi.org/10.1016/S0065-2164(07)62005-X
  12. Kim, Y. H., S. W. Kang, J. H. Lee, H. I. Chang, C. W. Yun, H. D. Paik, C. W. Kang, and S. W. Kim (2007) High cell density fermentation of Saccharomyces cerevisiae JUL3 in fed-batch culture for the production of $\beta$ -Glucan. J. ind. Eng. Chem. 13: 153-158.
  13. Quintero, O., A. Amicarelli, F. Sciascio, G. Scaglia (2008) State estimation in alcoholic continuous fermentation of Zymomonas mobilis using recursive bayesian filtering: a simulation approach. Bioresources. 3: 316-334.
  14. Chandel, A. K., E. S. Chan, R. Rudravaram, M. L. Narasu, L. V. Rao, and P. Ravindra (2007) Economics and environmental impact of bioethanol production technologies: an appraisal. Biotechnology and Molecular Biology Review 2: 14-32.
  15. Pauline M. D. (2008) Bioprocess Engineering Principles. pp. 446-472. World Science Press, Dongjak, Seoul, Korea.
  16. Wingren, A., M. Galbe, and G. Zacchi (2003) Technoeconomic evaluation of producing ethanol from softwood: comparison of SSF and SHF and identification of bottlenecks. Biotechnol. Prog. 19: 1109-1117.
  17. Hinman N. D., D. J. Schell, C. J. Riley, P. Bergeron, and P. J. Walter (1992) Preliminary estimate of the cost of ethanol production for SSF technology. Appl. Biochem. Biotechnol. 34-35: 639-649. https://doi.org/10.1007/BF02920584
  18. Han, H. J. and S. J. Kim (2006) Isolation and characterization of a strain for economical ethanol production. Korean J. Biotechnol. Bioeng. 21: 267-272.
  19. Yang, L. (2009) Continuous Ethanol Production by the Synchronous Saccharification and Fermentation using Food Wastes. MS. Thesis. Chonnam National University, Gwangju, Korea.
  20. Shafaghat, H., G. D. Najafpour, P. S. Rezaei, and M. Sharifzadeh (2009) Growth kinetics and ethanol productivity of Saccharomyces cerevisiae PTCC 24860 on various carbon sources. World Applied Sci. J. 7: 140-144.
  21. Thomas, M. W., and K. M. Bhat (1998) Methods for measuring cellulase activities, Method Enzymol. 160: 87-112.
  22. Park, B. G., W. G. Lee, Y. K. Chang, and H. N. Chang (1999) Long-term operation of continuous high cell density culture of Saccharomyces cerevisiae with membrane filtration and on-line cell concentration monitoring. Bioprocess Eng. 21: 97-100.
  23. Na, J. B. and J. S. Kim (2008) The optimum condition of SSF to ethanol production from starch biomass, Korean. Chem. Eng. Res. 46: 858-862.
  24. Kwon, J. K., H. S. Moon, J. S. Kim, S. W. Kim, and S. I. Hong (1999) Fed-batch simultaneous saccharification and fermentation of waste paper to ethanol. Korean J. Biotechnol. Bioeng. 14: 24-30.