Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Anti-obesity Effect of Rhizoma Atractylodis Herbal Acupuncture in High Fat Diet-induced Obese ICR Mouse

고지방 식이로 유도된 비만 생쥐에서 창출약침의 항비만 효과

  • Youh, Eun-Joo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Seo, Byung-Kwan (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Nam, Sang-Soo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Kang, Sung-Keel (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University)
  • 유은주 (경희대학교 한의과대학 침구학교실) ;
  • 서병관 (경희대학교 한의과대학 침구학교실) ;
  • 남상수 (경희대학교 한의과대학 침구학교실) ;
  • 강성길 (경희대학교 한의과대학 침구학교실)
  • Received : 2010.10.19
  • Accepted : 2010.11.30
  • Published : 2010.12.20
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Abstract

Objectives : The aim of this study was to investigate the anti-obesity potential and mechanisms of action of Rhizoma Atractylodis(RA) herbal acupuncture in high fat diet- induced obese ICR mice. Methods : Sample solutions for herbal acupuncture were prepared from the Rhizoma Atractylodis water extract powder at concentration of 150mg/kg and 300mg/kg with distilled water. Five week-old ICR mice acclimatized to the laboratory environment for 1 week were allocated into four groups: regular diet group (RD), high fat diet group(HFD), groups fed HFD with 150mg/kg RA herbal acupuncture treatment (RAE 150) and with 300mg/kg RA herbal acupuncture treatment(RAE 300). Herbal acupuncture groups were injected with either 150mg/kg or 300mg/kg of Rhizoma Atractylodis(RA) subcutaneously onto both Sinsu($BL_{23}$) alternately on the same time everyday for 30days. Body weight, gross appearance of epididymal fat area, blood glucose, insulin, insulin resistance(HOMA-IR), non-esterified fatty acid, cholesterol, triglyceride, AST, ALT, histological analysis of white adipose tissue, gene expression responsible for adipocyte differentiation and AMPK activation were analyzed. Results : RA herbal acupuncture inhibited the development of weight gain, hyperglycemia, hyperinsulinemia, hyperlipidemia, increases of AST and ALT, and the enlargement of fat cell size induced by HFD. Also, RA herbal acupuncture inhibited the expression of PPAR-${\gamma}$, C/$EBP{\alpha}$, aP2, LPL, FAS, SCD-1 and enhanced the activation of AMP-activated protein kinase. Conclusions : The results of this study demonstrate that RA herbal acupuncture can exert the anti-obesity effect and it is partially mediated by activation of AMPK and inhibition of the gene expressions responsible for adipocyte differentiation. Further studies will be required to ascertain the nti-obesity effect and mechanisms of action of RA herbal acupuncture in animal models and human for aclinical application.

Keywords

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