Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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EFFECT OF FLUORIDE IN NATURAL POLYMER ON ENAMEL DEMINERALIZATION

천연고분자 화합물을 이용한 불소겔이 법랑질 탈회에 미치는 영향

  • Kim, Ji-Yeon (Department of Pediatric Dentistry, School of Dentistry, Chosun University) ;
  • Lee, Sang-Ho (Department of Pediatric Dentistry, School of Dentistry, Chosun University) ;
  • Lee, Nan-Young (Department of Pediatric Dentistry, School of Dentistry, Chosun University)
  • 김지연 (조선대학교 치의학전문대학원 소아치과학교실) ;
  • 이상호 (조선대학교 치의학전문대학원 소아치과학교실) ;
  • 이난영 (조선대학교 치의학전문대학원 소아치과학교실)
  • Published : 2010.02.26
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Abstract

We have developed a sodium fluoride containing gelatin and methyl cellulose gel. Cariostatic abilities of those gel were investigated and compared with APF gel and fluoride varnish($Cavityshield^{TM}$). We prepared the bovine tooth samples and divided into two surface, control side and experimental side in same specimen for exclusion of difference between specimens. The experiment was consisted of 4 groups : (I) APF gel : (II) $Cavityshield^{TM}$ : (III) Gelatin F gel : (IV) Methyl cellulose F gel Decalcification were produced by placing each specimen into artificial acidic solution(pH 4.0) for 72 hours. Surface microhardness were measured and depth of demineralization lesion were measured by polarizing light microscope. The results were as follows: 1. The difference of VHN between control and experimental side is smallest in group I (p<0.05). 2. The largest difference was shown in group II (p<0.05). 3. There were no significant difference between group III and IV in microhardness test (p>0.05). 4. The difference of lesion depth is smallest in group I (p<0.05). 5. There were no significant difference between group II, III and IV in lesion depth (p>0.05). The result of the present study indicate that the fluoride containing gelatin and methyl cellulose gel is more effective than APF gel and is similar to fluoride varnish application for prevention of demineralization.

본 연구는 젤라틴과 메틸셀룰로오스를 기저재로 한 불소겔을 제작하고 이들의 높은 점조도와 표면에 흡착하는 능력이 치면에 불소를 오랜 시간 저류시켜 법랑질 탈회를 효과적으로 억제할 수 있는지 살펴보고자 시행되었다소의 전치로 시편 80개를 제작하였으며, (1) APF gel (2) $CavityShield^{TM}$, (3) Gelatin F gel (4) Methyl cellulose F gel 이렇게 4개의 군으로 분류하였다. 모든 시편을 인공우식용액에 72시간동안 보관하여 법랑질을 탈회시킨 후, 표면미세경도 측정법과 편광 현미경하에서 병소의 탈회 깊이를 측정하는 두 가지 방법을 사용하여 탈회정도를 측정하였다. 연구결과는 다음과 같다. 1) 불소를 처리한 experimental side와 처리하지 않은 control side간 미세경도의 차이는 APF를 적용한 제1군에서 71.82(VHN)로 가장 작은 것으로 나타났다(p<0.05). 2) $CavityShield^{TM}$를 도포한 제2군에서 148.24(VHN)로 가장 큰 차이를 보여 불소바니쉬의 탈회억제효과가 가장 큰 것으로 나타났다(p<0.05). 3) Gelatin과 methyl cellulose F gel을 도포한 3군과 4군에서 control side와 experimental side간 미세경도의 차이는 1군보다는 높고 2군보다는 낮았으나 3군과 4군간에 유의한 차이는 없었다(p>0.05). 4) 인공탈회병소의 깊이를 측정한 결과 APF를 처리한 제1군에서 control side와 experimental side간 병소의 깊이차이는 $16.69\;{\mu}m$로 가장 적게 나타났다(p<0.05). 5) 2,3,4군 에서는 병소깊이 측정을 통해 탈회억제효과가 APF군에 비해 우수하게 나타났으나(p<0.05) 각 군 간에 차이는 검증되지 않았다(p>0.05). 이상의 결과를 종합하여 보면 실험적으로 제조된 두 가지 불소겔은 APF gel에 비해 법랑질 탈회 억제효과가 우수하다고할 수 있으며 이는 APF gel에 비해 gelatin이나 methyl cellulose 의 높은 점조도가 불소를 더 장시간 치면에 접촉시킨 것으로 생각된다. 현재 가장 우수한 치면 잔류시간을 보이는 것으로 인정되는 불소바니쉬와 비교하여도 이들의 효과가 크게 떨어지지 않는 것을 알 수 있었으며 이는 향후 천연고분자 화합물을 이용한 다양한 불소제제의 개발 가능성을 보여주는 것이라 사료된다.

Keywords

References

  1. Knuston JW, Armstrong W : The effect of topically applied sodium fluoride on dental caries experience. Publ Health Rep, 58:1701-1715, 1943. https://doi.org/10.2307/4584683
  2. Adair SM : Current fluoride therapy in dentistry for children. Curr Opin Dent, 1: 583-591,1991.
  3. Ten Cate JM : In vitro sudies on effects of fluoride on de- and remineralization. J Dent Res, 69(sp Issue):614-619, 1990.
  4. Mellberg JR, Nicholson CR, Trubman A : The acquisition of fluoride by tooth enamel in vivo from selfapplied APF gel and prophylaxis paste. Caries Res, 7:173-178, 1973. https://doi.org/10.1159/000259841
  5. Cobb HB, Rozier RG, Bawden JW : A clinical study of the caries preventive effects of an APF solution and APF thixotropic gel. Pediatr Dent, 2:263-266, 1980.
  6. Hicks MJ, Flaitz CM, Garcia-Godoy F : Root-surface caries formation: effect of in vitro APF treatment. J Am Dent Assoc, 129:449-453, 1998.
  7. Kula K, Nelson S, Thompson V : In viro effect of APF gel on three composite resin. J Dent Res, 62:846-849, 1983. https://doi.org/10.1177/00220345830620071901
  8. 최인혁, 김은정, 김현정 등. : APF gel이 심미수복재료의 표면에 미치는 영향. 대한소아치과학회지, 33:281-289, 2006.
  9. Beltra'n Aguilar ED, Goldstein JW, Lockwood SA : Fluoride varnishes. A review of their clinical use, cariostatic mechanism, efficacy and safety. J Am Dent Assoc, 131:589-596, 2000.
  10. Wu H, Zhang ZX, Zhao HP, et al. : Preparation of sodium fluoride-loaded gelatin microspheres, characterization and cariostatic studies. J Microencapsul, 21:889-903, 2004. https://doi.org/10.1080/02652040400015445
  11. Gabre P, Ellefsen B, Birkhed D, et al. : Oral retention of fluoride from a mucosa adhesive paste (Orabase) supplemented with NaF - a pilot study. Oral Health Prev Dent, 3:159-163, 2005.
  12. Toumba KJ, Curzon ME : A clinical trial of a slowreleasing fluoride device in children. Caries Res, 39:195-200, 2005. https://doi.org/10.1159/000084798
  13. Reijden WA, Buijs MJ, Damen JJ, et al. : Influence of polymers for use in saliva substitutes on de- and remineralization of enamel in vitro. Caries Res, 31:216-223, 1997. https://doi.org/10.1159/000262403
  14. Gaffar A, Hunter CM, Mirajkar YR : Applications of polymers in dentifrices and mouthrinses. J Clin Dent, 13:138-148, 2002.
  15. 최후균, 이용복, 사홍기 등 : 신기술의약품개발. 도서출판 신일상사, 131-151, 2002.
  16. 이범진, 한건 : 제형의원리와기술. 도서출판신일상사, 200-449, 2000.
  17. White DJ, Featherstone JD : A longitudinal microhardness analysis of fluoride dentifrice effects on lesion progression in vitro. Caries Res, 21:502-512, 1987. https://doi.org/10.1159/000261059
  18. 설재헌, 오유향, 이난영 등 : 레이저 형광법을 이용한 인접면 우식증의 진단. 대한소아치과학회지, 31:236-246, 2004.
  19. Featherstone JD, ten Cate JM, Shariati M, et al. : Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res, 17:385-391, 1983. https://doi.org/10.1159/000260692
  20. 문성권, 이재천, 김영재 등 : Fluoride varnish와 Acidulated phosphate fluoride gel이 인공우식병소에 미치는 영향에 대한 미세전산화 단층촬영을 이용한 연구. 대한소아치과학회지, 31:212-222, 2004.
  21. Attin T, Buchalla W, Hellwig E : Effect of topical fluoride application on toothbrushing abrasion of resin composites. Dent Mater, 22:308-313, 2006. https://doi.org/10.1016/j.dental.2005.04.023
  22. Ekstrand J, Koch G, Lindgren LE, et al. : Pharmacokinetics of fluoride gels in children and adults. Caries Res, 15:213-220, 1981. https://doi.org/10.1159/000260517
  23. Hausen H : Benefits of topical fluorides firmly established. Evid Based Dent, 5:36-37, 2004. https://doi.org/10.1038/sj.ebd.6400250
  24. American Dental Association Council on Scientific Affairs. : Professionally applied topical fluoride: evidence- based clinical recommendations. J Am Dent Assoc, 137:1151-1159, 2006.
  25. Arends J, Schuthof J : Fluoride content in human enamel after fluoride application and washing - an in vitro study. Caries Res, 9:363-372, 1975. https://doi.org/10.1159/000260178
  26. LeCompte EJ, Whitford GM : The biologic availability of fluoride from alginate impressions and APF gel applications in children. J Dent Res, 60:776-780, 1981. https://doi.org/10.1177/00220345810600040201
  27. Whitford GM : Fluoride in dental products: safety considerations. J Dent Res, 66:1056-1060, 1987. https://doi.org/10.1177/00220345870660051501
  28. Garcia-Godoy F, Hicks MJ, Flaitz CM, et al. : Acidulated phosphate fluoride treatment and formation of caries-like lesions in enamel: effect of application time. J Clin Pediatr Dent, 19:105-110, 1995.
  29. Delbem AC, Cury JA : Effect of application time of APF and NaF gels on microhardness and fluoride uptake of in vitro enamel caries. Am J Dent, 15:169-172, 2002.
  30. Vaikuntam J : Fluoride varnishes: should we be using them? Pediatr Dent, 22:513-516, 2000.
  31. Chu CH, Lo EC : A review of sodium fluoride varnish. Gen Dent, 54:247-253, 2006.
  32. Bawden JW : Fluoride varnish: a useful new tool for public health dentistry. J Public Health Dent, 58:266-269, 1998. https://doi.org/10.1111/j.1752-7325.1998.tb03007.x
  33. Schmidt HF : The fluoride varnish procedure and possibilities of its utilization for the prevention of caries. Zahnarztl Mitt, 59:633-636, 1969.
  34. Bottenberg P, Bultmann C, Graber HG : Distribution of fluoride in the oral cavity after application of a bioadhesive fluoride-releasing tablet. J Dent Res, 77:68-72, 1998. https://doi.org/10.1177/00220345980770010701
  35. Bottenberg P, Cleymaet R, de Muynck C, et al. : Development and testing of bioadhesive, fluoridecontaining slow-release tablets for oral use. J Pharm Pharmacol, 43:457-464, 1991. https://doi.org/10.1111/j.2042-7158.1991.tb03514.x
  36. Vivien-Castioni N, Gurny R, Baehni P, et al. : Salivary fluoride concentrations following applications of bioadhesive tablets and mouthrinses. Eur J Pharm Biopharm, 49:27-33, 2000. https://doi.org/10.1016/S0939-6411(99)00041-7
  37. Croll TP, Nicholson JW : Glass ionomer cements in pediatric dentistry: review of the literature. Pediatr Dent, 24:423-429, 2002.
  38. De Moor RJ, Verbeeck RM : Effect of encapsulation on the fluoride release from conventional glass ionomers. Dent Mater, 18:370-375, 2002. https://doi.org/10.1016/S0109-5641(01)00052-5
  39. Creanor SL, Al-Harthy NS, Gilmour WH, et al. : Fluoride release from orthodontic cements-effect of specimen surface area and depth. J Dent, 31:25-32, 2003. https://doi.org/10.1016/S0300-5712(02)00085-4
  40. Mizrahi B, Domb AJ : Mucoadhesive tablet releasing iodine for treating oral infections. J Pharm Sci, 96:3144-3150, 2007. https://doi.org/10.1002/jps.20876
  41. Todd MA, Staley RN, Kanellis MJ, et al. : Effect of a fluoride varnish on demineralization adjacent to orthodontic brackets. Am J Orthod Dentofacial Orthop, 116:159-167, 1999. https://doi.org/10.1016/S0889-5406(99)70213-1
  42. Gokalp S, Baseren M : Use of laser fluorescence in monitoring the durability and cariostatic effects of fluoride and chlorhexidine varnishes on occlusal caries : a clinical study. Quintessence Int, 36:183-189, 2005.
  43. Arends J, Schuthof J, Jongebloed WG : Lesion depth and microhardness indentations on artificial white spot lesions. Caries Res, 14:190-195, 1980. https://doi.org/10.1159/000260453