Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Morphological Characteristics and Germination as Affected by Low Temperature and GA in Orostachys 'Jirisan' and 'Jejuyeonhwa' Seeds, Korea Native Plant

지리산바위솔과 제주연화바위솔 종자의 형태특성 및 저온과 GA에 대한 발아 반응

  • Received : 2010.05.11
  • Accepted : 2010.09.13
  • Published : 2010.12.31
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Abstract

This study was conducted to improve the seed germination by low temperature and $GA_3$ in $Orostachys$ $japonicus$ A. Berger (Jirisan) and $O.$ $iwarenge$ (Mak.) Hara (Jejuyeonhwa), Korean native plants. Observation of the seeds using a stereo microscope showed that all seeds of the two species have wrinkled surface and oblong shape. Seed size ranged 0.77-1.00/0.25-0.37 mm (length/width), indicating that the seeds are minute seeds. When the seeds of two $Orostachsis$ species were sown into petri-dish and placed in a plant growth chamber of 10, 15, 20, or $25^{\circ}C$, 'Jirisan' showed seed germination below 20% at all temperatures and 'Jejuyeonhwa' 80% at only $10^{\circ}C$. Seed germination of 'Jirisan' increased up to 44% at $10^{\circ}C$ by low temperature ($4^{\circ}C$) storage for 10 days, but decreased again at storage for more than 20 days. The seeds of 'Jejuyeonhwa' showed a large increase in seed germination by low temperature for 20-30 days, which was 95% at $10^{\circ}C$, but low temperature for more than 40 days significantly decreased seed germination. Dipping treatment in GA3 solution of $50-400mg{\cdot}L^{-1}$ for different periods (3, 6, 12, and 24 hrs) remarkably improved germination rate and speed in both species, 80-100% in 'Jirisan' and 90-100% in 'Jejuyeonhwa' at all concentrations and dipping times used in this study.

우리나라 자생식물의 하나인 지리산바위솔($O.$ $japonicus$ A. Berger)과 제주연화바위솔[$O.$ $iwarenge$ (Mak.) Hara]의 종자 형태를 관찰하고 종자발아생리를 구명하기 위해 저온과 GA를 처리하였다. 종자의 형태를 실체현미경으로 관찰한 결과 2가지 바위솔 모두 세로로 주름진 쌀알 모양이었으며, 크기는 길이 0.77-1.00mm, 폭 0.25-0.37mm로 미세 종자임이 확인되었다. 휴면유무와 발아최적 온도를 구명하기 위해, Petri-dish에 파종후 온도가 다른 파종상에서 발아 시켰을 때, 지리산바위솔은 모든 온도에서 20% 이하의 발아율을 보였고, 제주연화바위솔은 $10^{\circ}C$에서만 80% 정도의 발아율을 보였다. $4^{\circ}C$ 냉장고에 건식저장을 하면서 10일 간격으로 온도별로 파종한 결과, 지리산바위솔은 무처리에서는 발아가 전혀 보이지 않았다. 10일간 저온처리 후 $10^{\circ}C$ 파종상에 두었을 때 발아율 44%로 많이 향상되었으나, 그 이상의 저온 처리에서는 발아율이 다시 감소하였다. 제주연화바위솔의 경우 저온 처리 30일까지는 발아율이 크게 향상되어 $10^{\circ}C$에서 95% 발아율을 보였다. 그러나 그 이상의 저온처리(40일)에서는 발아율이 급격히 저하되었다. $GA_3$ 농도를 $50-400mg{\cdot}L^{-1}$으로 하고, 침지시간은 3-24시간 범위로 하였을 때, 두가지 바위솔 모두 발아 속도가 현저하게 빨라지고 발아율이 획기적으로 높아지는 결과를 얻었다. 지리산바위솔은 모든 침지 시간에서 80-100% 발아율을 보였다. 제주연화바위솔도 $GA_3$ 농도와 시간에 상관 없이 파종 후 6일만에 90-100%의 발아율을 나타내었다.

Keywords

References

  1. Amen, R.D. 1967. The effect gibberellic acid and stratification on the seed dormancy and germination in Luzula Spicata. Physiol. Plant. 20:6-12. https://doi.org/10.1111/j.1399-3054.1967.tb07135.x
  2. Ball, V. 1998. Ball redbook (16th ed.), Ball publishing, Illinois, USA, p. 413-414.
  3. Bewley, J.D. and M. Black. 1982. The release from dormancy. p.127-198. In J.D. Bewley and M. Black (eds.). Physiology and biochemistry of seeds. Springer-Verlag. Berlin, Heideberg, Germany.
  4. Cho, D.H., C.H. Park, B.J. Park, S.E. Shin, K.C. Lee, C.Y. Yu, and S.D. Ahn. 1997. Effects of growth regulators, temperature, and light on the germination in wild vegetable (Chwinamul) seeds. Kor. J. Plant Res. 10:39-44.
  5. Choi, G.S., K.W. Park, and H.M. Kang. 2003. Effect of temperature, light condition, and priming treatment on the germination of Aster glehni FR. seed. J. Bio-environ. Cont. 12:132-138.
  6. Choi, S.O. and M.S. Yang. 1992. Some biochemical component changes during the culture of Orostachys japonicus A. Berger. Kor. J. Plant Tissue Culture 19:209-212.
  7. Heyenga, A.G., J.A. Lucas, and P.M. Dewick. 1990. Production of tumor inhibitory lignins in callus cultures of Podophyllum hexandrum. Plant Cell Rep. 9:382-385.
  8. Jeong, H.W. 1991. Studies on the propagation on Korea native Aralia (Aralia elata Seemann). MS thesis. Kon-Kuk Univ., Seoul. Korea
  9. Kang, J.H., J.S. Park, and J.W. Kim. 1995. Effect of long-day and night-break treatment on growth and anthesis of Orostachys japonicus A. Berger. Kor. J. Medical Crop Sci. 40:600-607.
  10. Kang, J.H., Y.S. Ryu, and B.G. Cho. 1996. Effect of night-break period on growth and anthesis of Orostachys japonicus. Kor. J. Medical Crop Sci. 41:236-242.
  11. Kang, J.H., Y.J. Kim, and B.S. Jeon. 2001. Effects of presown cold stratification, $KNO_{3}$ and acetone treatment on germination of Crotalaria sessiflora L. Kor. J. Medical Crop Sci. 9: 124-129.
  12. Kang, J.H., S.H. Jeon, S.Y. Yoon, D.O. Hong, and S.C. Shin. 2005. Effect of different temperatures on growing and flowering of Orostachys japonicus A. Berger. Kor. J. Medical Crop Sci. 13:186-189.
  13. Kim, H.D. and K.R. Park. 2005. Genetic variation in five species of Korean Orostachys (Crassulaceae). Kor. J. Plant Taxonomy 35:295-311.
  14. Kim, K.S. 1992. Dormancy and seed germination characteristics in Aralia continentalis Kitagawa. MS thesis. Seoul Nat. Univ., Seoul. Korea.
  15. Kunjun, F. and H. Ohba. 2001. Crassulaceae. In Flora of china. Wu, Z. and P.H. Raven (eds.). Missouri botanical garden press, St. Louis. p. 202-268.
  16. Kwon, T.R., J.H. Jo, Y.S. Kwon, S.P. Lee, and B.S. Choi. 1993. Study on seed treatments to facilitate germination of some wild edible greens. RDA. J. Agri. Sci. 35:416-421.
  17. Lee, C.B. 1987. Plant taxonomy. Sin-Go Hyangmoonsa. Korea. p. 223-224.
  18. Lee, C.H and N.Y. Han. 1994. Effect of cold treatment on seed germination in Viola species native to Korea. J. Kor. Soc. Hort. Sci. 12:342-343.
  19. Lee, K., H. Kim, and K. Park. 2003. Numerical taxonomy of Korean Orostachys (Crassulaceae). Kor. J. Taxon. 33:359-371.
  20. Ohba, H. 1990. Notes towards a monograph of genus Orostachys (Crassulaceae). J. Jpn. Bot. 65:1-11.
  21. Ohba, H. 2003. Orostachys. in illustrated handbook of succulent plants: Crassulaceae. Eggli, U. (ed.), Springer-Verlag, Heidelberg. p. 186-190.
  22. Park, K.W., K.W. Park, Y.J. Lee, and H.S. Choi. 1996. Studies of seed germination of some wild greens and effect of chilling treatment on germination. J. Kor. Sci. Hort. 14 (Suppl. II): 148-149. (Abstr.)
  23. Park, K.W., G.P. Lee, K.W. Park, and J.C. Jeong. 1998. Seed morphology of thirty Korean wild green species and effect of seed stratification on germination. Hort. Environ. Biotechnol. 39:129-134.
  24. Salisbury, F.B. and C.W. Ross. 1992. Hormones and growth regulators: auxins and gibberellin. p. 485-503. In F.B. Salisbury and C.W. Ross ($4^{th}$ eds.). Wadsworth Publishing Co., Belmont, CA, USA.
  25. Shin, D.Y., Y.M. Lee, and H.J. Kim. 1994. Anatomy and artificial seed propagation in anti-cancer plant Orostachys japonicus A. Berger, Kor. J. Crop Sci. 39:146-157.
  26. Yang, Y.J. and Y.S. Kim. 1993. Seed germination of Korean wild medicinal plants: Capsella bursapastoris, Persicaria perfoliata and Commelina communis. J. Kor. Soc. Hort. Sci. 34:315-319.
  27. Yoo, D.L., K.S. Cho, S.Y. Ryu, and I.C. Ryu. 1999. Effect of low temperature and gibberellin treatment for promoting seed germination on Hanabusaya asiatica. Kor. J. Soc. Hort. Sci. Tech. 17(Suppl. II):674. (Abstr.)
  28. Yoon, S.T., D.J. Lee, and Y.H. Kim. 1997. The technology for increasing seed viability by priming treatment and plant growth regulators in medicinal plants. Kor. J. Int. Agri. 11:85-95.