Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Analysis of Chemical Composition, Microstructure and Hydroxyapatite Structure for Mouse Teeth

생쥐 치아의 화학적 조성, 미세구조 및 Hydroxyapatite 구조 분석

  • Kim, Eun-Kyung (Graduate School of Analytical Science and Technology, Chungnam National University) ;
  • Jeon, Tae-Hoon (Graduate School of Analytical Science and Technology, Chungnam National University) ;
  • Kim, Chang-Yeon (Graduate School of Analytical Science and Technology, Chungnam National University) ;
  • Nam, Seung-Won (Department of Bioscience and Biotechnology, Chungnam National University) ;
  • Song, Kyung (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Lee, Sang-Gil (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kim, Youn-Joong (Graduate School of Analytical Science and Technology, Chungnam National University)
  • 김은경 (충남대학교 분석과학기술대학원) ;
  • 전태훈 (충남대학교 분석과학기술대학원) ;
  • 김창연 (충남대학교 분석과학기술대학원) ;
  • 남승원 (충남대학교 생명과학과) ;
  • 송경 (한국기초과학지원연구원 전자현미경연구부) ;
  • 이상길 (한국기초과학지원연구원 전자현미경연구부) ;
  • 김윤중 (충남대학교 분석과학기술대학원)
  • Received : 2010.08.28
  • Accepted : 2010.09.28
  • Published : 2010.09.30
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Abstract

The aim of this study is to determine microstructure, chemical composition and crystal structure of hydroxyapatite for mouth teeth using optical microscopy and electron microscopy as well as electron probe micro-analysis (EPMA). Enamel, a protective cover to the teeth, consisted of rods oriented in regular and had relatively higher crystallinity and Ca component. In contrast, dentin showed a sponge-like microstructure with circular holes which were passages of dentinal tubules, and had higher Mg component than the enamel region due to its higher organic content. Hydroxyapatite crystals appeared as large rods in enamel, but as small needles in dentin. Their electron diffraction patterns were different by their crystallinity as well as by the organic content of the matrix.

본 연구에서는 광학 및 전자현미경 관찰과 전자현미분석을 수행하여 쥐 치아의 미세구조, 화학 조성 및 hydroxyapatite의 결정구조를 파악하였다. 치아의 겉 표면을 둘러싸고 있는 법랑질은 막대들이 일정하게 배열된 구조이며 결정도가 높고 Ca이 많이 침착되어 있다. 그에 비해 상아질은 상아세관이 통과하는 원형의 구멍이 고루 분포되어 있는 스펀지 구조이며 유기질의 존재로 인해 Mg의 분포가 법랑질에 비해 상대적으로 높게 나타났다. Hydroxyapatite 결정들은 법랑질에서는 크기가 큰 막대 형태로 나타났으나 상아질에서는 크기가 작은 침상 형태로 나타났으며, 각 조직의 결정도와 유기질의 유무에 따라 전자회절패턴의 차이를 보였다.

Keywords

References

  1. Boyde A, Lester KS: An electron microscope study of fractures dentinal surfaces. Calcified Tissue Research 1 : 122-136, 1967. https://doi.org/10.1007/BF02008082
  2. Elliott JC: Calcium phosphate biominerals. In: Matthew JK, ed, Reviews in Mineralogy and Geochemistry. Phosphates, pp. 427- 453, Mineralogical Society of America, Chantilly, 2002.
  3. Fong S, White SN, Paine ML, Luo W, Snead ML, Sarikaya M: Enamel structure properties controlled by engineered proteins in transgenic mice. Journal of Bone and Mineral Research 18 : 2052-2059, 2003. https://doi.org/10.1359/jbmr.2003.18.11.2052
  4. Glauert AM: Practical methods in electron microscopy. American Elsevier, New York, pp. 1-198, 1974.
  5. Glimcher MJ: Bone: Nature of the calcium phosphate crystals and cellular, structural and physical chemical mechanism in their formation. In: Nita S, ed, Reviews in Mineralogy and Geochemistry. Medical Mineralogy and Geochemistry, pp. 223-282, Mineralogical Society of America, Chantilly, 2006.
  6. Habelitz S, Zartoshtimanesh S, Balooch M, Marshall SJ, Marshall GW, Denbesten PK: Structure and properties of murine and human dentin. Materials Research Society 874 : L5.18.1, 2005.
  7. Kinney JH, Nalla RK, Pople JA, Breunug TM, Ritchie RO: Agerelated transparent root dentin: mineral concentration, crystallite size and mechanical properties. Biomaterials 26 : 3363-3376, 2005. https://doi.org/10.1016/j.biomaterials.2004.09.004
  8. Leventouri T, Antonakos A, Kyriacou A, Venturelli R, Liarokapis E, Perdikatsis V: Crystal structure studies of human dental apatite as a function of age. International Journal of Biomaterials 698547, 2009.
  9. Mann S: Molecular recognition in biomineralization. Nature 332 : 119-124, 1988. https://doi.org/10.1038/332119a0
  10. Nalbandian J, Gonzales F, Sognnaes RF: Sclerotic age change in root dentin of human teeth as observed by optical, electron, and X-ray microscopy. Journal of Dental Research 39 : 598, 1960. https://doi.org/10.1177/00220345600390032101
  11. Nalla RK, Porter AE, Daraio C, Minor AM, Radmilovic V, Stach EA, Tomsia AP, Ritchie RO: Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy. Micron 36 : 672-680, 2005. https://doi.org/10.1016/j.micron.2005.05.011
  12. Nishizawa Y, Morii H, Durlach J: New perspectives in magnesium research. Springer, Japan, pp. 359, 2006.
  13. Oleynikov, P. eMap, a program for generating 3D Fourier maps and determining peak positions. AnaliTEX www.analitex.com/ eMap.html; Oleynikov, P. http://www.analitex.com.
  14. Olszta MJ, Cheng X, Jee SS, Kumar R, Kim YY, Kaufman MJ, Douglas EP, Gower LB: Bone structure and formation: A new perspective. Materials Science and Engineering R58 : 77-116, 2007.
  15. Palmer LC, Newcomb CJ, Kaltz SR, Spoerke ED, Stupp SI: Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chemical Review 108 : 4754-4783, 2008. https://doi.org/10.1021/cr8004422
  16. Porter AE, Nalla RK, Minor A, Jinschek JR, Kisielowski C, Radmilovic V, Kinney JH, Tomsia AP, Ritchie RO: A transmission electron microscopy study of mineralization in age-induced transparent dentin. Biomaterials 26 : 7650-7660, 2005. https://doi.org/10.1016/j.biomaterials.2005.05.059
  17. Suk DH, Kim CY: Determination of age in humans from root dentin transparency. Korean Journal of Oral Medicine 7 : 59-65, 1982. (Korean)
  18. Tamerler C, Sarikaya M: Molecular biomimetics: genetic synthesis, assembly and formation of materials using peptides. MRS Bulletin 5 : 504-512, 2008.
  19. Tjaderhane L, Hietala EL, Larmas M: Mineral element analysis of carious and sound rat dentin by electron probe microanalyzer combined with back-scattered electron image. Journal Dental Research 74 : 1770-1774, 1995. https://doi.org/10.1177/00220345950740110901
  20. White SN, Luo W, Paine ML, Fong H, Sarikaya M, Snead ML: Biological organization of hydroxyapatite crystallites into a fibrous continuum toughens and controls anisotropy in human enamel. Journal Dental Research 80 : 321-326, 2001. https://doi.org/10.1177/00220345010800010501
  21. Zou XD, Sukharev Y, Hovmöller S: ELD-a computer program system for extracting intensities from electron diffraction patterns. Ultramicroscopy 49 : 147-158, 1993. https://doi.org/10.1016/0304-3991(93)90221-I