Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High Prevalence of Fluoroquinolone- and Methicillin-Resistant Staphylococcus pseudintermedius Isolates from Canine Pyoderma and Otitis Externa in Veterinary Teaching Hospital

  • Yoo, Jong-Hyun (BK21 Basic and Diagnostic Veterinary Specialist Program for Animal Diseases and Department of Veterinary Internal Medicine, Konkuk University) ;
  • Yoon, Jang-W. (Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine) ;
  • Lee, So-Young (BK21 Basic and Diagnostic Veterinary Specialist Program for Animal Diseases and Department of Veterinary Internal Medicine, Konkuk University) ;
  • Park, Hee-Myung (BK21 Basic and Diagnostic Veterinary Specialist Program for Animal Diseases and Department of Veterinary Internal Medicine, Konkuk University)
  • Received : 2009.10.30
  • Accepted : 2009.12.18
  • Published : 2010.04.28
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Abstract

Recently, a total of 74 Staphylococcus pseudintermedius isolates were collected from clinical cases of canine pyoderma and otitis externa in Korea. In this study, we examined in vitro fluoroquinolone resistance among those isolates using a standard disc diffusion technique. The results demonstrated that, except for one isolate, approximately 18.9% to 27.0% of the isolates possessed bacterial resistance to both veterinary- and human-licensed fluoroquinolones including moxifloxacin (18.9% resistance), levofloxacin (20.3% resistance), ofloxacin (24.3% resistance), ciprofloxacin (25.7% resistance), and enrofloxacin (27.0% resistance). Most surprisingly, 14 out of 74 (18.9%) isolates were resistant to all the five fluoroquinolones evaluated. Moreover, a PCR detection of the methicillin resistance gene (mecA) among the 74 isolates revealed that 13 out of 25 (52.0%) mecApositive isolates, but only 7 out of 49 (14.3%) mecA-negative isolates, were resistant to one or more fluoroquinones. Taken together, our results imply that bacterial resistance to both veterinary- and human-use fluoroquinolones becomes prevalent among the S. pseudintermedius isolates from canine pyoderma and otitis externa in Korea, as well as that the high prevalence of the mecA-positive S. pseudintermedius isolates carrying multiple fluoroquinolones resistance could be a potential public health problem.

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References

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