Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Synthesis of an Amphiphilic Poly(${\gamma}$-Glutamic Acid)-Cholesterol Conjugate and Its Application as an Artificial Chaperone

  • Lee, Eun-Hye (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Kamigaito, Yoshiki (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Tsujimoto, Takashi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Uyama, Hiroshi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Sung, Moon-Hee (Department of Bio & Nanochemistry, Kookmin University)
  • Received : 2010.06.03
  • Accepted : 2010.06.29
  • Published : 2010.10.28
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Abstract

A poly(${\gamma}$-glutamic acid) (${\gamma}$PGA)-cholesterol conjugate was synthesized and its properties were then evaluated. The conjugate exhibited an amphiphilic nature derived from the hydrophilic ${\gamma}$PGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles, becoming an aqueous solution when at low concentrations, and at high concentrations the result was the formation of a physical gel. By utilizing the self-aggregating properties of the conjugate in water, an artificial chaperone was developed. A complex of protein, with the nanoparticles of the conjugate, was formed and the protein was released upon the dissociation of the nanoparticles through the addition of ${\beta}$-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.

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References

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